Novel method for tissue culture rapid propagation of acer paimatum

A technology of the Acer palmatum group and a new method, which is applied in the biological field, can solve the problems of low cutting propagation coefficient, unfavorable industrial production, and increased production costs, and achieve the effects of shortening the propagation time, convenient operation, and low cost

Active Publication Date: 2013-11-27
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN101849506A discloses a rapid propagation method of Acer palmatum by tissue culture. The method uses NN69 as the basic medium. After a series of sterilization processes, the stems of Acer palmatum are cultured in vitro and controlled by hormones to establish The rapid propagation system of Acer palmatum has successfully cultivated complete rooted regenerated plants, and the trans

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  • Novel method for tissue culture rapid propagation of acer paimatum
  • Novel method for tissue culture rapid propagation of acer paimatum
  • Novel method for tissue culture rapid propagation of acer paimatum

Examples

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Example Embodiment

[0018] Example 1: A method for tissue culture and rapid propagation of Acer with chicken feet, which comprises the following steps:

[0019] S1. explant explants: collect disease-free young stems of Acer, cut into 2cm-long stems, rinse in running water for 5 minutes, and absorb water;

[0020] S2. Sterilization: put the rinsed explants into the ultra-clean workbench, disinfect with 70% alcohol for 13s, rinse with sterile water for 4 times, and then put them into 0.1% mercuric chloride for sterilization 5min, rinse 4 times with sterile water, and place on sterile paper to dry;

[0021] S3. Start-up culture: inoculate the sterilized explants in a start-up medium for culture, wherein the start-up medium is WPM+GA 3 0.1mg / L+TDZ 0.05 mg / L+sucrose 30g / L+agar 5g / L, pH 5.5, culture conditions: light intensity 2800LX, light time 14h, temperature 23°C, culture time 14d, axillary bud elongation after initiation of culture 2cm;

[0022] S4. Rooting culture: choose the stem segment o...

Example Embodiment

[0023] Example 2: A method for tissue culture and rapid propagation of Acer with chicken feet, which comprises the following steps:

[0024] S1. Cut out explants: collect disease-free young stems of Acer chinensis, cut them into 3 cm long stems, rinse in running water for 10 minutes, and absorb water;

[0025] S2. Sterilization: put the rinsed explants into the ultra-clean workbench, disinfect with 75% alcohol for 18s, rinse with sterile water for 6 times, and then put them into 0.2% mercuric chloride for sterilization 10min, rinse with sterile water 6 times, and place on sterile paper to dry;

[0026] S3. Start-up culture: inoculate the sterilized explants in a start-up medium for culture, wherein the start-up medium is WPM+GA 3 0.1mg / L+TDZ 0.05mg / L+sucrose 30g / L+agar 5g / L, pH 6.5, culture conditions: light intensity 3200LX, light time 18h, temperature 25°C, culture time 21d, axillary bud elongation after initiation of culture 4cm;

[0027] S4. Rooting culture: choose t...

Example Embodiment

[0028] Example 3: A method for tissue culture and rapid propagation of Acer with chicken feet, which comprises the following steps:

[0029] S1. Cut out explants: collect disease-free young stems of Acer, cut into 2.5cm-long stems, rinse in running water for 8 minutes, and absorb water;

[0030] S2. Sterilization: put the rinsed explants into the ultra-clean workbench, disinfect with 73% alcohol for 15s, rinse with sterile water for 5 times, and then put them into 0.2% mercuric chloride for sterilization 8min, rinse with sterile water 5 times, and place on sterile paper to dry;

[0031] S3. Start-up culture: inoculate the sterilized explants in a start-up medium for culture, wherein the start-up medium is WPM+GA 3 0.1mg / L+TDZ 0.05mg / L+sucrose 30g / L+agar 5g / L, pH 5.5~6.5, culture conditions: light intensity 3000LX, light time 15h, temperature 24°C, culture time 18d, after starting the culture, axillary buds Elongate 3cm;

[0032] S4. Rooting culture: select the stem segme...

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Abstract

The invention discloses a novel method for tissue culture rapid propagation of acer paimatum . The novel method comprises the steps as follows: S1, explant cutting: collecting and cutting an acer paimatum young and tender stalk section to be 2-3 cm in length; S2, sterilization, placing an explant on an extra-clean workbench, and sterilizing the explant with alcohol and mercuric chloride; S3, initiation culture: inoculating the sterilized explant to an initiation culture medium for culture, wherein the initiation culture medium is provided with WPM, 30.1mg/L of GA, 0.05mg/L of TDZ, 30g/L of cane sugar and 5g/L of agar; S4, rooting culture, after the initiation culture, selecting an acer paimatum stalk section with buds, wherein the acer paimatum stalk section is 1-2 cm in length; cutting off and transferring axillary buds to a rooting culture medium for culture, and the rooting culture medium is provided with 1/2WPM, 0.05mg/L of NAA, 25g/L of cane sugar and 5g/L of agar. According to the invention, the culture media are simple in formula and low in cost; only via the initiation culture and the rooting culture, an acer paimatum aseptic seedling can be rapidly propagated from the stalk section of the explant of the acer paimatum, so that the propagation time is remarkably shortened; the novel method is convenient to operate, can increase the propagation base number for a short time, and provides technique support for industrial seedling-raising.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new method for rapid propagation of Acer palmatum tissue culture. Background technique [0002] Acer paimatum (Acer paimatum), also known as Yafeng, belongs to the genus Aceraceae, and is a valuable ornamental tree species in urban landscapes. It is native to the Yangtze River Basin in China, and is also distributed in Japan and the Korean Peninsula. my country is mainly distributed in the provinces of the Yangtze River Basin, and it mostly grows in forest margins or sparse forests of mountains and hills below 1200m above sea level. Acer palmatum is a deciduous small tree, about 8m high, with dark gray bark, purple or lavender green branches in the current year; light gray, purple or dark purple branches in perennial branches. Acer palmatum leaves opposite, 7-10 cm in diameter, truncate at base, deeply lobed palmately, with 5-9 lobes, usually 7-lobed, lobes ovate-oblo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 冯爱云王小辉吴佳川沈香兰朱晓菲
Owner SICHUAN COLORLINK CO LTD
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