Novel method for tissue culture rapid propagation of acer paimatum

Abstract

The invention discloses a novel method for tissue culture rapid propagation of acer paimatum . The novel method comprises the steps as follows: S1, explant cutting: collecting and cutting an acer paimatum young and tender stalk section to be 2-3 cm in length; S2, sterilization, placing an explant on an extra-clean workbench, and sterilizing the explant with alcohol and mercuric chloride; S3, initiation culture: inoculating the sterilized explant to an initiation culture medium for culture, wherein the initiation culture medium is provided with WPM, 30.1mg / L of GA, 0.05mg / L of TDZ, 30g / L of cane sugar and 5g / L of agar; S4, rooting culture, after the initiation culture, selecting an acer paimatum stalk section with buds, wherein the acer paimatum stalk section is 1-2 cm in length; cutting off and transferring axillary buds to a rooting culture medium for culture, and the rooting culture medium is provided with 1 / 2WPM, 0.05mg / L of NAA, 25g / L of cane sugar and 5g / L of agar. According to the invention, the culture media are simple in formula and low in cost; only via the initiation culture and the rooting culture, an acer paimatum aseptic seedling can be rapidly propagated from the stalk section of the explant of the acer paimatum, so that the propagation time is remarkably shortened; the novel method is convenient to operate, can increase the propagation base number for a short time, and provides technique support for industrial seedling-raising.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a new method for rapid propagation of Acer palmatum tissue culture. Background technique [0002] Acer paimatum (Acer paimatum), also known as Yafeng, belongs to the genus Aceraceae, and is a valuable ornamental tree species in urban landscapes. It is native to the Yangtze River Basin in China, and is also distributed in Japan and the Korean Peninsula. my country is mainly distributed in the provinces of the Yangtze River Basin, and it mostly grows in forest margins or sparse forests of mountains and hills below 1200m above sea level. Acer palmatum is a deciduous small tree, about 8m high, with dark gray bark, purple or lavender green branches in the current year; light gray, purple or dark purple branches in perennial branches. Acer palmatum leaves opposite, 7-10 cm in diameter, truncate at base, deeply lobed palmately, with 5-9 lobes, usually 7-lobed, lobes ovate-oblo...

AI Technical Summary

Problems solved by technology

Chinese patent CN101849506A discloses a rapid propagation method of Acer palmatum by tissue culture. The method uses NN69 as the basic medium. After a series of sterilization processes, the stems of Acer palmatum are cultured in vitro and controlled by hormones to establish The rapid propagation system of Acer palmatum has successfully cultivated complete rooted regenerated plants, and the transplanting survival rate has reached 90%. Industrialized production of Acer palmatum seedlings, but this method uses NN69 as the basic medium, and the hormone KT-30 needs to be added to the medium, which increases production costs, and the explants have to go through start-up culture, subculture, rooting culture, and production. The cycle is long, which is still not conducive to factory production

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