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Method for extracting T-Cadinol from Cryptomeria japonica

A technology for juniperol and cedar, which is applied in the field of extracting T-juniperol, can solve problems such as the lack of disclosure of a method for extracting T-juniperol, and achieve the effects of simple preparation process, low operating cost, and low investment.

Inactive Publication Date: 2013-11-27
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is no disclosure of a method for extracting T-kininol from Japanese cedar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 2kg Japanese cedar leaves were crushed, passed through a 60-mesh sieve, and placed in supercritical CO 2 In the extractor, the extraction pressure is 12.8MPa, the extraction temperature is 24°C, and the extraction time is 80min. 6MPa, temperature 45℃; system CO 2 The flow rate is 15L / h. Collect Japanese cedar extract. Add 0.5% activated carbon to the Japanese cedar extract and stir to decolorize, filter, add water to disperse the filtrate, put it into a polyamide resin column, first elute with 25% ethanol by volume until the effluent is colorless, and then use 60% by volume ethanol Elute with ethanol solution, collect 60% ethanol eluate, concentrate under reduced pressure, extract the concentrate with ether, combine ether extracts, concentrate under reduced pressure, and dry to obtain 2.1 g of T-cadinol.

Embodiment 2

[0020] 2kg Japanese cedar leaves were crushed, passed through a 60-mesh sieve, and placed in supercritical CO 2 In the extractor, the extraction pressure is 24.7MPa, the extraction temperature is 40°C, and the extraction time is 120min. 4MPa, temperature 30℃; system CO 2 The flow rate is 19L / h. Collect Japanese cedar extract. Add 1.0% activated carbon to the Japanese cedar extract and stir to decolorize, filter, add water to disperse the filtrate, add it to a polyamide resin column, first elute with 40% ethanol by volume until the effluent is colorless, and then use 80% by volume ethanol Elute with ethanol solution, collect 80% ethanol eluent, concentrate under reduced pressure, extract the concentrated solution with ether, combine the ether extracts, concentrate under reduced pressure, and dry to obtain 1.9 g of T-cadinol.

Embodiment 3

[0022] 2kg Japanese cedar leaves were crushed, passed through a 60-mesh sieve, and placed in supercritical CO 2 In the extractor, the extraction pressure is 19.7MPa, the extraction temperature is 35°C, and the extraction time is 60min. 10MPa, temperature 35℃; system CO 2 The flow rate is 20L / h. Collect Japanese cedar extract. Add 1.5% activated carbon to the Japanese cedar extract and stir to decolorize, filter, add water to disperse the filtrate, put it into a polyamide resin column, first elute with 30% ethanol until the effluent is colorless, and then use 75% ethanol Elute with ethanol solution, collect 75% ethanol eluent, concentrate under reduced pressure, extract the concentrated solution with ether, combine the ether extracts, concentrate under reduced pressure, and dry to obtain 2.9 g of T-cadinol.

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PUM

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Abstract

The invention discloses a method for extracting T-Cadinol from Cryptomeria japonica. The method comprises steps of: crushing leaves of Cryptomeria japonica; carrying out supercritical CO2 extraction to obtain an extract; adding active carbon for decolorization; enriching an obtained decolorization solution by a polyamide resin column chromatography; condensing the destaining solution and then extracting the destaining solution with ether; and drying to obtain a T-Cadinol extractive. The preparation method has high yield and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine chemistry, and relates to a method for extracting T-ginol from Japanese cedar. Background technique [0002] T-cadinol (T-Cadinol), a sesquiterpenoid compound, molecular formula C 15 h 26 O, relative molecular weight 222.37, oily amorphous solid, mp44-46℃, structural formula: [0003] . [0004] T-gininol is widely distributed in the olive family (Bureseraceae) plant Myrrha ( Commiphora guidottii Chiov ) resin, Compositae (Compositae) plant plane grass ( Eupatoriem odoratum L. ), Lamiaceae (Labiatae) plant Spanish sage ( Salvia hispanica ), Taxodiaceae (Taxodiaceae) Alpine fir ( Athrotaxis selaginoides Don ) heartwood, Japanese cedar ( Cryptomeria japonica (L.f.) D.Don ) leaves and Taiwan fir ( Taiwania cryptomerioides (Hayata) ) of the wood. Pharmacological studies have shown that T-cadinol has a smooth muscle relaxation effect. It has a relaxing effect on the contract...

Claims

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Application Information

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IPC IPC(8): C07C35/36C07C29/76
CPCY02P20/54
Inventor 张金芳万冬梅
Owner NANJING BIAOKE BIO TECH
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