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Method for preparing poecilobdella manillensis medium-high activity anticoagulant crude product

A high-activity technology of Hirudo phenanthrene, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, medical raw materials derived from leeches/worms, etc., can solve the problem of low anticoagulant activity content of dry product powders, and achieve The content of natural anticoagulant substances is large, the effect is good, and the effect of reducing blood viscosity

Active Publication Date: 2013-12-04
安堂娥
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing methods of directly using leeches to make dry products, the whole method is basically used as a medicine, and most of them adopt the traditional drying or freeze-drying method and then pulverized. There is no temperature control in most links during the implementation process, and the final dry product powder low anticoagulant activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 20kg of healthy Hirudo phenanthae over 1 year old, cut the head of Hirudo phenanthae mechanically or manually (the front 1 / 3 part of the trunk of Hirudo phenanthae and contains the salivary glands of Hirudo phenanthae), and get 6kg of raw materials, according to the weight ratio Mix it with a sodium chloride solution with a mass concentration of 0.8% at a ratio of 1:4, and place the mixture in a cold room at 4-6°C to allow the temperature of the mixture to reach 4-6°C;

[0019] Use a colloid mill with a cooling device to repeatedly grind the mixture in step and control the temperature at 4-6°C (the highest temperature in the whole process of the same batch does not exceed 10°C), the mixture is in the colloid mill Repeated grinding each time the residence time does not exceed 30s, and the rotation speed is above 2800n / min, so that the raw material of Hirudo phenanthae in the mixture becomes a slurry of 2-50μm, and then the ground slurry is placed in a cold room at...

Embodiment 2

[0023] Take 20kg of healthy Hirudo phenanthae over 1 year old, cut the head of Hirudo phenanthae mechanically or manually (the front 1 / 3 part of the trunk of Hirudo phenanthae and contains the salivary glands of Hirudo phenanthae), and get 6kg of raw materials, according to the weight ratio Mix it with a sodium chloride solution with a mass concentration of 1.0% at a ratio of 1:4, and place the mixture in a cold room at 4-6°C to allow the temperature of the mixture to reach 4-6°C;

[0024] Use a colloid mill with a cooling device to repeatedly grind the mixture in step and control the temperature at 4-6°C (the highest temperature in the whole process of the same batch does not exceed 10°C), the mixture is in the colloid mill Repeated grinding each time the residence time does not exceed 30s, and the rotation speed is above 2800n / min, so that the raw material of Hirudo phenanthae in the mixture becomes a slurry of 2-50μm, and then the ground slurry is placed in a cold room at...

Embodiment 3

[0028] Take 20kg of healthy Hirudo phenanthae over 1 year old, cut the head of Hirudo phenanthae mechanically or manually (the front 1 / 3 part of the trunk of Hirudo phenanthae and contains the salivary glands of Hirudo phenanthae), and get 6kg of raw materials, according to the weight ratio Mix it with a sodium chloride solution with a mass concentration of 0.8% at a ratio of 1:6, and place the mixture in a cold room at 4-6°C to allow the temperature of the mixture to reach 4-6°C;

[0029] Use a colloid mill with a cooling device to repeatedly grind the mixture in step and control the temperature at 4-6°C (the highest temperature in the whole process of the same batch does not exceed 10°C), the mixture is in the colloid mill Repeated grinding each time the residence time does not exceed 30s, and the rotation speed is above 2800n / min, so that the raw material of Hirudo phenanthae in the mixture becomes a slurry of 2-50μm, and then the ground slurry is placed in a cold room at...

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Abstract

The invention discloses a method for preparing a poecilobdella manillensis medium-high activity anticoagulant crude product. The method includes the steps of selecting the parts, containing most anticoagulant, of poecilobdella manillensis living bodies, breaking tissue cell walls and smashing animal tissue fiber in a superfine mode through grinding to increase extraction amount of anticoagulation active ingredients, meanwhile, effectively controlling the low-temperature environment to guarantee operation of key technical steps, and finally obtaining the crude product with very high anticoagulant activity content. Through measurement according to a thrombin titration in the leech item in the 'Chinese pharmacopoeia' (2010 edition), the antithrombase activity content is larger than or equal to 1200.0U. The poecilobdella manillensis medium-high activity anticoagulant crude product obtained by the method can be made into preparations such as capsules and tablets. Due to the fact that the poecilobdella manillensis medium-high activity anticoagulant crude product is high in natural anticoagulant substance content, the poecilobdella manillensis medium-high activity anticoagulant crude product can effectively improve blood microcirculation, dissolves blood clots, lowers uric acid, lowers blood viscosity, lowers blood fat and is good in cardiovascular and cerebrovascular disease curative effect.

Description

technical field [0001] The invention belongs to the technical field of extracting Hirudo phenanthae, and in particular relates to a method for preparing a crude product of a high-activity anticoagulant in Hirudo phenanthae. Background technique [0002] The medical hirudaceae animal, Hirudo sylvestris, has been successfully included in the second volume (2011 edition) of the Zhuang Pharmacopoeia of Quality Standards of Zhuang Medicine of Guangxi Zhuang Autonomous Region, which provides a basis for the legal use of Hirudo spp. as a drug. The Guangxi Zhuang Pharmacopoeia stipulates that the anticoagulant activity of 1 gram of dried leeches should not be less than 220.0U; the 2010 edition of "Chinese Pharmacopoeia" stipulates that the anticoagulant activity of 1 gram of dried leeches should not be less than 16.0U. The dry anticoagulant activity of leech and willow leaf leech is not less than 3.0U. The medicine produced according to the Pharmacopoeia standard has a low anticoag...

Claims

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Application Information

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IPC IPC(8): A61K35/62A61K38/58A61P9/00A61P7/02
Inventor 宋延坤覃壬许振朝黄俊安堂娥张雄胜
Owner 安堂娥
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