Open-type constant-current diffusion protein crystallization array chip and manufacturing method thereof

Abstract

The invention relates to an open constant current diffusion protein crystallization array chip and its manufacturing method, which is especially suitable for biological process research and new drug design. It includes a substrate and a substrate; there are several protein crystallization units on the substrate; the substrate and the substrate There is an annular microfluidic main channel on the combined plane; the annular microfluidic main channel communicates with the protein crystallization unit through the microfluidic diffusion channel; the annular microfluidic main channel is connected in series and forms a single column crystallization unit together with the protein crystallization unit in the middle array, or a multi-row crystallization unit array composed of several single-row crystallization unit arrays connected in parallel. The invention has the advantages of fast protein crystallization speed and high crystal cluster density; the protein crystals formed in the crystallization unit are not subjected to shear force and can be extracted and analyzed conveniently; the protein consumption is small, the operation is flexible and applicable High-pass screening of crystallization conditions; the fabrication of the chip is simple, the structure is compact, the cost is low, and it can be mass-produced.

Description

technical field [0001] The invention relates to an open constant current diffusion protein crystallization array chip and its manufacturing method, which is a device for directly, effectively and comprehensively understanding the high-level structure and function of proteins through protein crystallization, and is especially suitable for biological process research and new drug design , belonging to the field of biotechnology. Background technique [0002] The study of protein structure and function is of great significance to the understanding of life processes at the molecular level and the design of new drugs. The crystal analysis of protein is the most direct and effective method to fully understand the three-dimensional structure of protein, so it has attracted widespread attention. [0003] In order to efficiently obtain protein crystals that can be used for crystal analysis, it is necessary to use a small amount of protein solution for reasonable condition screening ...

AI Technical Summary

Problems solved by technology

[0008] A common problem in the above experiments is that when the volume of the liquid is constant, the total amount of protein molecules contained is very limited, and it is difficult to promote the crystallization of protein molecules

If the concentration of protein is increased, the crystallization efficiency can be increased, but the obtained protein crystals are small in size and low in cluster density, so it is difficult to be directly used for diffraction analysis

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