Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method for stem cell preparation capable of preventing and treating mycoplasma pneumoniae of swine

A technology of mycoplasma pneumoniae and stem cell preparations, which is applied to cells modified by introducing foreign genetic material, medical preparations containing active ingredients, antibacterial drugs, etc., which can solve the problems of easy drug resistance and high residue of antibiotic drugs

Inactive Publication Date: 2013-12-11
武汉市畜牧兽医科学研究所
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the embodiments of the present invention is to provide a preparation method of a stem cell preparation that can prevent and treat mycoplasma pneumonia in pigs, aiming to solve the current problems of antibiotics that are prone to drug resistance and high residue in pigs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for stem cell preparation capable of preventing and treating mycoplasma pneumoniae of swine
  • Preparation method for stem cell preparation capable of preventing and treating mycoplasma pneumoniae of swine
  • Preparation method for stem cell preparation capable of preventing and treating mycoplasma pneumoniae of swine

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0049] Specific examples provided by the invention are as follows:

[0050]Under sterile conditions, the primary porcine leg bone cover was completely separated, the tibia and femur were mechanically squeezed to obtain bone marrow, and porcine bone marrow mesenchymal stem cells were isolated from the collected bone marrow by the Ficol gradient separation method, and 1×10 5 Inoculated at 12-well cell culture plate at a density of 12 / mL, the medium used was DMEM liquid medium (containing 10% FBS and 100U / mL penicillin, 100U / mL streptomycin), placed at 37°C, 5% CO 2 cultured in an incubator. After the cells covered the bottom of the culture dish, the cells were digested with 0.25% trypsin-EDTA solution, and a paving stone-like subtype of cells was isolated from them by the differential attachment method for further culture. When the cell confluence reaches more than 85%, inoculate it in a new culture dish and store it at 37°C, 5% CO 2 Cultivate in the incubator for 24 hours, an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method for a stem cell preparation capable of preventing and treating mycoplasma pneumoniae of swine. The preparation method comprises the steps of separating swine bone marrow mesenchymal stem cells from newborn swine leg bone marrows; separating a subtype cell BMSC-S3 cell line of the swine bone marrow mesenchymal stem cells; establishing a BMSC-S3 / PBD-1 cell line stably transfected by PBD-1 gene; detecting genes expressed by BMSC-S3 / PBD-1 cells by using PCR; and detecting antigen molecules expressed by the BMSC-S3 / PBD-1 cells by using cellular immunofluorescence chemical analysis and flow cytometry. The BMSC-S3 / PBD-1 cells prepared by the method can stably express PDB-1 and has the characteristics of standard BMSC stem cells. The BMSC-S3 / PBD-1 cells stably transfected by the PBD-1 gene are injected into a swine body suffering from mycoplasma pneumoniae, with an effective dosage of 1-5*10<5> per time per kg of weight, so that mycoplasma pneumoniae diseases of the swine can be controlled. The BMSC-S3 / PBD-1 cells can be stored in a liquid nitrogen container for a long time, so that sources can be guaranteed. The stem cell preparation provided by the invention has no drug resistance; and foreign protein is processed only by the swine body, without risk of drug residues, so that the stem cell protein has wide application prospects.

Description

Technical field [0001] The invention is a field of biological preparation preparation, and especially involves a method of preparation of stem cell preparations that can prevent and treat piglet pneumonia. Background technique [0002] Pig pneumonia, commonly known as pig asthma, is a chronic contact respiratory disease caused by mycoplasma hy Hyopneumoniae (MHP). It has the characteristics of high incidence and low mortality. [0003] Most of the newly introduced pig groups are mostly acute. The incidence and mortality rate often above 20 %, and the most acute mortality rate can be as high as 80 % to 100 %.After the disease, chronic dry cough, slow growth, and delayed development can cause reduction of pigs' immunity after infection, which leads to mixed in other pathogens such as thoroughbacter bacteria, pig breeding and respiratory syndrome virus, pneumonia Bacteria and other mixed bacteria.Infection causes Percineres Piratory Disease Complex (PRDC) or other diseases.Moreover,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/28C12N15/85C12N5/10A61P11/00A61P31/04
Inventor 黄海军高其双陈志华占才耀钱运国彭霞向敏周莉卢顺陶弼菲
Owner 武汉市畜牧兽医科学研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com