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Method for rapidly screening iron-rich rice

A screening method and rice technology, applied in the direction of chemical reaction of materials and material analysis by observing the effect on chemical indicators, can solve problems such as time-consuming, cumbersome sample pre-processing procedures, breeding work services, etc. , to achieve the effect of low cost and accurate cost

Inactive Publication Date: 2013-12-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination methods of mineral content in rice generally require expensive instruments and cumbersome sample pretreatment procedures. The sample ashing process generally takes 24-36 hours, and the conventional ashing furnace can only put 36-36 hours at a time. 48 samples, ashing samples are few and ashing time is long, and subsequent determination by element analyzer or ICP-MS is costly and time-consuming, and the mineral content of rice cannot be determined quickly and efficiently, so it cannot be timely and effective to serve the breeding work

Method used

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  • Method for rapidly screening iron-rich rice
  • Method for rapidly screening iron-rich rice
  • Method for rapidly screening iron-rich rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Rapid screening method for iron-rich rice, using the high-iron mutant of the Zhenong No. 7 rice variety (2009M6, stored in the Rice Breeding Office of the Department of Agronomy, Zhejiang University) as the rice to be tested, and the conventional Zhenong No. 7 variety as the standard product ; After the rice to be tested and the standard rice are shelled, the mature grains of the rice to be tested (referred to as the rice grains to be tested) and the mature grains of the standard rice (referred to as the standard rice grains) are obtained respectively; Paddy rice grain and standard product rice grain carry out the following operations respectively:

[0040] 1) Put 4 rice grains into a 10mL glass test tube, then add 1mL of NaOH solution with a mass concentration of 4%, gelatinize in a constant temperature incubator at 35°C for 6h, take it out and vibrate on an oscillator for 2min, the oscillation frequency 300~350r / min;

[0041] 2) Add 0.5mL of 2M hydrochlori...

Embodiment 2、93－11

[0059] Example 2. Screening of 93-11 mutants with high iron content

[0060] Using BPT staining method in 1428 copies of 93-11 rice M 2 31 high-iron mutant materials were initially screened out, accounting for 2.2% of the total materials. After further screening in the next three generations, the ratio of mutant materials to the total materials showed an upward trend, and the ratios were 29.3%, 46.7% and 75.4% respectively. ;Among the 1132 mutated Zhenong No. 7 materials, 21 high-iron mutant materials were initially screened, accounting for 1.9% of the total materials. After further screening for the next 4 generations, the ratio of mutants to the total materials gradually increased ; in F of 1556 hybrid materials 3 107 high-iron mutants were screened out, accounting for 6.9% of the total materials. Compared with the above two mutagenesis materials, the mutation rate was higher. Iron content is lower. As with the above two mutant materials, the breeding material F 2 ~F 7 ...

Embodiment 3

[0061] Example 3. The 2009-223 (black rice) variety (a mutant of the seed coat color of 93-11) was used as the rice to be tested, and the conventional 93-11 was used as the standard product, and the detection was carried out as in Example 1.

[0062] The result is as follows: the color of the reactant of the rice grain to be tested is obviously darker than the color of the reactant of the standard product rice grain.

[0063] Verification test:

[0064] The specific experimental process is as follows:

[0065] (1) Experimental instruments and reagents

[0066] Iron standard solution (Aladdin Reagent Company); 4% sodium hydroxide solution; 4,7-diphenyl 1,10-phenanthroline (BPT) staining solution: 0.1% ethanol solution; emulsifier OP (OP -10) Solution: 20% aqueous solution; acetic acid-sodium acetate buffer solution (pH 4.5): Weigh 50 g of anhydrous sodium acetate and dissolve it in an appropriate amount of water, add 47.5 mL of glacial acetic acid, and dilute to 1 L to obtain...

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Abstract

The invention discloses a method for rapidly screening iron-rich rice. The method for rapidly screening the iron-rich rice takes rice obtained by mutating or improving on the basis of standard rice as rice to be detected and comprises the steps of respectively carrying out the following steps 1) to 4) on grains of the rice to be detected and grains of the standard rice: 1) carrying out gelatinization on the grains; 2) adding hydrochloric acid into gelatinized grains obtained in the step 1) for neutralizing; 3) adding a hydroxylammonium chloride aqueous solution, an acetic acid-sodium acetate buffer solution, an emulsifier OP aqueous solution and the like, oscillating, uniformly shaking and then standing; 4) adding BPT staining solution into mixture obtained in step 3), uniformly shaking and then standing, and separating clear liquid at the upper layer; 5) comparing colour of reactant of the grains of the rice to be detected with that of reactant of the grains of the standard rice, and if the colour of the reactant of the grains of the rice to be detected is deeper than that of the reactant of the grains of the standard rice, iron content of the grains of the rice to be detected is higher than that of the grains of the standard rice.

Description

technical field [0001] The invention relates to a rapid screening method for iron content in rice grains, and mainly relates to the fields of agronomy, breeding and rice. Background technique [0002] Rice is the second largest food crop in the world and also the main food crop in my country. Its nutritional content is directly related to people's health. Iron is a trace element necessary for the growth and development of the human body. It is widely distributed in the human body. Iron deficiency will reduce the mental development of infants, reduce immunity, increase the mortality of mothers and infants, increase human fatigue, and reduce work efficiency. WHO (2004) reported that iron deficiency is a common nutritional problem in the world. About 4 to 5 billion people in the world (accounting for 66 to 80% of the world's population) suffer from iron deficiency, of which 2 billion anemia patients are caused by iron deficiency, especially in developing countries. Therefore,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 石春海王克敏吴建国
Owner ZHEJIANG UNIV
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