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Method for simultaneously analyzing multiple aromatic hazardous substances in food

A technology of harmful substances and aromatics, which is applied in the field of simultaneous analysis of multiple aromatic harmful substances in food, can solve the problems of large sample consumption and cumbersome methods, and achieve the effects of simplifying experimental operations, shortening extraction time, and shortening degreasing time

Active Publication Date: 2013-12-11
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a method for simultaneously analyzing multiple aromatic harmful substances in food, that is, a method for simultaneously extracting and preparing PAHs, phenolic compounds and aromatic amines in food, so as to solve the problem of cumbersome methods in the prior art and sample consumption. Large volume and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]1) Extraction and preparation of PAHs sample solution: Cut 15g of barbecue sample into pieces and put it into an Erlenmeyer flask, 1ml of internal standard (deuterated benzopyrene concentration is 3ng / ml, deuterated chrysene concentration is 1ng / ml, deuterated acenaphthene The concentration is 2ng / ml, the deuterated-1-aminonaphthalene concentration is 2μg / ml and the deuterated-4-aminobiphenyl concentration is 0.4 μg / ml;), 29ml cyclohexane, ultrasonic extraction in water bath for 20min, let stand for 5min , take 10ml of the extract and filter the membrane, take 3ml of the extract and load it on the activated Si phase solid phase extraction column for enrichment and purification, and collect the effluent ①; use 1ml of cyclohexane extract to rinse the solid phase extraction column, collect the Washing solution ②; combine the effluent ① and eluent ② into a test tube, add DMSO for liquid-liquid extraction, then take out the lower layer solution with a straw for use, continue t...

Embodiment 2

[0043] 1) Extraction and preparation of PAHs sample solution: Cut 15g of smoked fish sample into pieces and put it into an Erlenmeyer flask, add 1ml of internal standard (the concentration of deuterated benzopyrene is 3ng / ml, the concentration of deuterated chrysene is 1ng / ml, The concentration of deuterated acenaphthene is 2ng / ml, the concentration of deuterated-1-aminonaphthalene is 2 μg / ml and the concentration of deuterated-4-aminobiphenyl is 0.4 μg / ml;), 29ml cyclohexane, ultrasonic extraction in water bath for 20min, Stand still for 5 minutes, take 10ml of the extract and filter the membrane, take 3ml of the extract and load it on the activated Si-phase solid-phase extraction column for enrichment and purification, and collect the effluent ①; use 1ml of cyclohexane extract to rinse the solid-phase extraction column , collect the eluent ②; combine the effluent ① and eluent ② into a test tube, add DMSO for liquid-liquid extraction, then take out the lower layer solution wit...

Embodiment 3

[0051] 1) Extraction and preparation of PAHs sample solution: Cut 15g fried egg sample into pieces and put it into an Erlenmeyer flask, add 29ml cyclohexane, 1ml internal standard (the concentration of deuterated benzopyrene is 3ng / ml, the concentration of deuterated chrysene 1ng / ml, the concentration of deuterated acenaphthene is 2ng / ml, the concentration of deuterated-1-aminonaphthalene is 2μg / ml and the concentration of deuterated-4-aminobiphenyl is 0.4 μg / ml;), ultrasonic extraction in water bath for 20min, Stand still for 5 minutes, take 10ml of the extract and filter the membrane, take 3ml of the extract and load it on the activated Si-phase solid-phase extraction column for enrichment and purification, and collect the effluent ①; use 1ml of cyclohexane extract to rinse the solid-phase extraction column , collect the eluent ②; combine the effluent ① and eluent ② into a test tube, add DMSO for liquid-liquid extraction, then take out the lower layer solution with a straw fo...

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PUM

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Abstract

The invention aims at providing a method for simultaneously analyzing multiple aromatic hazardous substances in food, that is, a method for simultaneously extracting and preparing PAHs (Polycyclic Aromatic Hydrocarbons), phenolic compounds and aromatic amine in food. According to the method, sampling of only one time is needed, the sample consumption is reduced, the inspection time is shortened, the use amount of reagents is saved, secondary pollution is reduced at the same time, and the service life of a HPLC (High Performance Liquid Chromatography) chromatographic column is prolonged; as target detected substances are extracted by using an organic solvent ultrasonic extraction method, the extraction time of the target detected substance is greatly shortened; steps for extracting and preparing a sample liquid of PAHs in food are simplified, and organic solvent liquid-liquid extraction-reextraction degreasing is utilized in the PHAs detection process, so that the degreasing time is greatly shortened, and the experimental operation is simplified.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and specifically relates to a method for simultaneously analyzing multiple aromatic harmful substances in food, that is, a method for simultaneously extracting, preparing and detecting three types of samples of polycyclic aromatic hydrocarbons, phenolic compounds and aromatic amines in food Methods. Background technique [0002] Food undergoes extremely complex chemical and physical changes during high-temperature processing, and its composition is extremely complex. There are harmful and / or toxic components in these compounds, and the aromatic harmful substances are mainly polycyclic aromatic hydrocarbons, phenolic compounds and aromatic amines. The three types of substances, polycyclic aromatic hydrocarbons, phenolic compounds, and aromatic amines, have different chemical properties, and the methods of extracting and preparing test samples are also different. [0003] Polycyclic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08
Inventor 齐祥明陈玉新邓雅婧巩文萍
Owner OCEAN UNIV OF CHINA
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