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Applications of icaritin in preparing medicament for preventing and treating alzheimer disease

A technology of icariin and medicine, which is applied in the field of application of icariin in the preparation of drugs for preventing and treating Alzheimer's disease, can solve the problems that icariin has not yet been used to prevent and treat Alzheimer's disease, and achieve good results. Market application prospect, prevention and treatment of Alzheimer's disease, effect of reducing phosphorylation

Active Publication Date: 2013-12-18
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the use of icariin in the prevention and treatment of Alzheimer's disease

Method used

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  • Applications of icaritin in preparing medicament for preventing and treating alzheimer disease
  • Applications of icaritin in preparing medicament for preventing and treating alzheimer disease
  • Applications of icaritin in preparing medicament for preventing and treating alzheimer disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The effects of icariin on GSK-3 and its downstream protein β-catenin were investigated in 3T3-L1 cells by protein detection and cotton-coat fluorescence test. The method is as follows:

[0027] Protein detection: 3T3-L1 cells were purchased from the American Type Culture Collection (ATCC: CL-173), using DMEM medium containing 10% fetal bovine serum, at 37°C and 5% CO 2 cultured under conditions. After the cells were inoculated and fused, add icariin (purchased from Nanjing Zelang Pharmaceutical Technology Co., Ltd., CAS NO: 38226-86-7), lithium chloride (LiCl) and corresponding solvent (control) and incubate for 15 minutes, then extract the protein Specifically, ultrasonic lysis was performed for 2 minutes in a cold lysis solution (50mM Tris, pH7.4, 150mM NaCl, 1mM EDTA, 1%TritonX-100, 0.1%SDS, and a mixture of protease inhibitors), and then Perform protein detection.

[0028] Immunofluorescence test: After cell inoculation and fusion, change to adipogenic induction ...

Embodiment 2

[0033] Take 3g of icariin, 75g of medicinal starch and 20g of microcrystalline cellulose respectively, pass the medicinal starch through a 60-mesh sieve, then mix evenly with icariin and microcrystalline cellulose, pass through a 60-mesh sieve, and pack into 1 No. capsule shells, made into 1000 capsules; each capsule contains 3mg icariin. Take orally, 3 times a day, 1 capsule each time.

[0034] According to the detection standard of hollow capsules in Chinese Pharmacopoeia, the appearance, water content, microbial inspection, disintegration time, weight difference, content and related substances of the prepared capsules all meet the requirements.

Embodiment 3

[0036] Take 300g of icariin, 600g of hypromellose, 1kg of sodium starch glycolate, 800g of microcrystalline cellulose, 11.5kg of lactose and 100g of magnesium stearate, mix well, and add 10% Starch slurry, granulated, dried at 50-60°C for 5 hours, sieved with a 20-mesh sieve, compressed into tablets, with a theoretical tablet weight of 143 mg, made into 100,000 tablets, each containing 3 mg of icariin. Take orally, 3 times a day, 1 capsule each time.

[0037] According to the testing standards of Chinese Pharmacopoeia tablets, the appearance, water content, microbial inspection, dissolution rate, weight difference, content and related substances of the prepared tablets all meet the requirements.

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Abstract

The invention discloses applications of icaritin in preparing a medicament for preventing and treating alzheimer disease. The study on the influence of the icaritin on GSK-3 (glycogen synthase kinase-3) and a downstream protein beta-catenin thereof in 3T3-L1 cells through protein detection and immunofluorescent test discovers that the icaritin can promote the nuclear transfer of beta-catenin, reduce Tau protein phosphorylation, protect the activity of neuronal cells and inhibit the neurofibrillary tangle by inhibiting the activity of the GSK-3 through blood brain barrier for the first time, so that the icaritin plays a role in preventing and treating the alzheimer disease. As a novel GSK-3 inhibitor coming from natural products, the icaritin has excellent market application prospects.

Description

【Technical field】 [0001] The invention belongs to the field of traditional Chinese medicines, and relates to a new medicinal application of icariin, an effective component of traditional Chinese medicines, in particular to the application of icariin in the preparation of drugs for preventing and treating Alzheimer's disease. 【Background technique】 [0002] Alzheimer's disease (AD) is a neurodegenerative disease, clinically manifested as progressive cognitive dysfunction. The characteristic neuropathological changes of AD patients mainly exist in senile plaques (senile plaques, SPs) outside the nerve cells and neurofibrillary tangles (neurofibrillary tangles, NFTs) in the cells, accompanied by the apoptosis of neurons and neurites. contact degradation, etc. SPs are mainly composed of β-amyloid (amyloid beta, Aβ) deposition, and NFTs are mainly composed of double-stranded helical filaments (paried helical filaments, PHFs) formed by abnormally hyperphosphorylated microtubule-a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/353A61P25/00A61P3/10A61P9/10A61P35/00A61P29/00A61P25/28
Inventor 王新峦秦岭王楠周虹曹会娟
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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