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Immunosorbent for treating dilated cardiomyopathy and preparation method thereof

A technology of dilated cardiomyopathy and immunosorbent, which is applied in the field of immunosorbent for the treatment of dilated cardiomyopathy and its preparation, can solve the problems of high price, ligand shedding, immune response, etc., and achieve low cost and easy storage , cheap effect

Active Publication Date: 2015-08-26
JAFRON BIOMEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the ligands of any immunosorbent have the possibility of falling off, and protein A, as a biologically active protein, is likely to cause immune reactions once it falls off as a ligand, and there are potential hidden dangers in treatment.
Therefore, the protein A immunosorbent has the disadvantages of high price and poor stability of the ligand. At the same time, protein A is a biologically active protein, and its ligand falls off easily causing immune reactions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of DCM immunosorbent for plasma adsorption is as follows:

[0032]Step 1: Preparation of Agarose Gel Balls:

[0033] Place a 500 mL three-necked flask equipped with a stirring device in a water bath at 60 °C, and add 145 mL of toluene, 55 mL of chloroform, and 5 mL of polysorbate-80 (Tween-80) into the bottle. Stir well and keep warm. Slowly pour 100 mL of the prepared agar solution with a mass fraction of 4% into a three-necked flask, and adjust the stirring speed to disperse the agar solution into droplets of appropriate size in the organic phase. After the dispersion is uniform (about 30min), remove the water bath, stir at room temperature and let the system cool down naturally. After the system was cooled, the organic solvent in the upper layer was discarded, the synthesized spherical agar gel carrier was washed with a large amount of water, the gel balls of 50-300 μm were screened out, washed several times with distilled water, and stored in ...

Embodiment 2

[0043] A preparation method of DCM immunoadsorbent for whole blood perfusion is as follows:

[0044] Step 1: Preparation of Agarose Gel Balls:

[0045] Place a 500mL three-necked flask equipped with a stirring device in a 60°C water bath, and add 145mL of toluene, 55mL of chloroform, and 805mL of Tween into the bottle. Stir well and keep warm. Slowly pour 100 mL of the prepared agar solution with a mass fraction of 6% into a three-necked flask, and adjust the stirring speed to disperse the agar solution into droplets of appropriate size in the organic phase. After the dispersion is uniform (about 30min), remove the water bath, stir at room temperature and let the system cool down naturally. After the system was cooled, the organic solvent in the upper layer was discarded, the synthesized spherical agar gel carrier was washed with a large amount of water, the gel balls of 300-800 μm were screened out, washed several times with distilled water, and stored in a wet state in a r...

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Abstract

Immunosorbent for treating dilated cardiomyopathy and preparation method thereof. Wherein, the immunosorbent for treating dilated cardiomyopathy includes a porous polymer carrier as an adsorbent matrix and a ligand fixed on the surface of the carrier by chemical crosslinking, wherein the ligand is 3-indolyl-BETA- D-Glucuronic acid sodium salt. The immunoadsorbent for treating dilated cardiomyopathy has the advantages of safety, non-toxicity, low cost and the like.

Description

technical field [0001] The invention relates to an immunosorbent for blood purification and a preparation method thereof, in particular to an immunosorbent for treating dilated cardiomyopathy and a preparation method thereof. Background technique [0002] It is estimated that the annual diagnosis rate of dilated cardiomyopathy (DCM) is about 8 / 100,000, and the prevalence rate is about 37 / 100,000. It is estimated that there are more than 500,000 patients in my country, and half of them are under the age of 55. The 5-year survival rate is only 40%. DCM is characterized by marked enlargement of the left ventricle (mostly) or right ventricle, accompanied by varying degrees of myocardial hypertrophy, ventricular systolic dysfunction, and cardiac enlargement, heart failure, arrhythmia, and embolism as the basic features. [0003] In recent years, scientists have found through research on the clinical and pathogenesis of DCM that the immune process mediated by autoantibodies causes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/48A61K31/7056A61P9/04
Inventor 董凡
Owner JAFRON BIOMEDICAL