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Preparation method of drug bearing microsphere/chitosan/sodium alginate injectable aquogel

A technology of drug-loaded microspheres and sodium alginate, which is applied in the field of preparation of biomedical materials, can solve the problems of poor drug release performance and low mechanical strength, and achieve the effects of enhanced mechanical strength, easy availability of raw materials, and weakened burst release effect

Active Publication Date: 2013-12-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hydrogels prepared by the above-mentioned prior art generally have the problems of low mechanical strength and poor drug release performance.

Method used

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  • Preparation method of drug bearing microsphere/chitosan/sodium alginate injectable aquogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Dissolve 0.35 g chitosan in 2% 10 ml dilute acetic acid aqueous solution to obtain solution 1; dissolve 0.3 g sodium alginate in 10 ml 80 ℃ deionized water to obtain solution 2; dissolve 0.03 g Tris Dissolve in 1 ml of deionized water to obtain solution 3; dissolve 0.75 g of anhydrous calcium chloride in 3 ml of deionized water to obtain solution 4;

[0023] (2) Stir the solutions 1, 2, and 3 obtained in step (1) at room temperature at 470 rpm to obtain a mixed solution; add 0.105 g of drug-loaded microspheres of 300-450 um prepared in advance to the mixed solution, and use The quality machine was dispersed evenly at a high speed of 7000 rpm to obtain a suspension, which was stored in a freezer at 4 °C for 2 h;

[0024] (3) Inject the suspension into the defect site, and after 7 min, after initial coagulation, inject 2.1 ml of solution 4; the drug-loaded hydrogel is obtained.

[0025] In vitro drug release curve experiment of composite hydrogel: add 2 mg drug-loade...

Embodiment 2

[0027] (1) Dissolve 0.1 g chitosan in 1% 10 ml dilute acetic acid aqueous solution to obtain solution 1; dissolve 0.12 g sodium alginate in 2 ml 70 ℃ deionized water to obtain solution 2; dissolve 0.05 g Tris Dissolve in 10 ml of deionized water to obtain solution 3; dissolve 1.5 g of anhydrous calcium chloride in 2 ml of deionized water to obtain solution 4;

[0028] (2) Stir the solutions 1, 2, and 3 obtained in step (1) at room temperature at 300 rpm to obtain a mixed solution; add 0.88 g of drug-loaded microspheres of 200-300 um prepared in advance to the mixed solution, and use the homogenizer The quality machine was 2000 rpm at high speed to disperse evenly to obtain a suspension, which was stored in a freezer at 0 °C for 4.5 h;

[0029] (3) Inject the suspension into the defect site, and after 3 min, after initial solidification, inject 1.47 ml of solution 4; the drug-loaded hydrogel is obtained.

[0030]The drug-loaded microspheres / chitosan / sodium alginate injectable ...

Embodiment 3

[0032] (1) Dissolve 0.18 g chitosan in 3% 10 ml dilute acetic acid aqueous solution to obtain solution 1; dissolve 0.15 g sodium alginate in 15 ml 60 ℃ deionized water to obtain solution 2; dissolve 0.75 g Tris Dissolve in 5 ml deionized water to obtain solution 3; dissolve 3 g anhydrous calcium chloride in 6 ml deionized water to obtain solution 4;

[0033] (2) Stir the solutions 1, 2, and 3 obtained in step (1) at room temperature at 600 rpm to obtain a mixed solution; add 0.6 g of drug-loaded microspheres of 100-200 um prepared in advance to the mixed solution, and use the homogenizer The quality machine was dispersed evenly at a high speed of 4700 rpm to obtain a suspension, which was frozen at 8 °C for 8 h;

[0034] (3) Inject the suspension into the defect site, and after 10 min, after initial coagulation, inject 6 ml of solution 4; the drug-loaded hydrogel is obtained.

[0035] The drug-loaded microspheres / chitosan / sodium alginate injectable hydrogel prepared in this e...

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Abstract

The invention discloses a preparation method of a drug bearing microsphere / chitosan / sodium alginate injectable aquogel. The preparation method comprises the following steps of: dissolving chitosan into a water solution of dilute acetic acid to obtain a solution 1; dissolving sodium alginate into hot deionized water to obtain a solution 2; dissolving Tris into deionized water to obtain a solution 3; dissolving anhydrous calcium chloride into deionized water to obtain a solution 4; uniformly mixing the solutions 1, 2 and 3 to obtain a mixed solution, and freezing and storing the mixed solution; dispersing preliminarily-prepared drug bearing microspheres into the mixed solution, and injecting the mixed solution on a defected part to initially form the aquogel; then, injecting the solution 4 to obtain a cured aquogel. The aquogel prepared by the using preparation method disclosed by the invention is rapid in forming, good in biocompatibility, high in drug encapsulation efficiency and little in brust release in an in-vitro drug release process; the preparation method is simple in process, easy in acquisition of raw materials, low in cost and easy for realization of industrialization.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and relates to the preparation technology of drug-loaded injectable materials, in particular to a preparation method of drug-loaded microspheres / chitosan / sodium alginate injectable hydrogel. Background technique [0002] Bone defects caused by trauma, tumor resection or congenital diseases have always been a major problem in the field of orthopedics. Bone grafting is a commonly used clinical operation. Traditionally, open surgery is often used, but it has the disadvantage of extensive stripping of bone defect and surrounding soft tissue, and destroying local blood circulation while bone grafting. In recent years, with the development of minimally invasive techniques With the development of minimally invasive bone grafting, due to the small surgical trauma, fewer complications, and the protection of local blood circulation, it is gradually being developed clinically. I...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/52A61L27/54
Inventor 魏坤许为康
Owner SOUTH CHINA UNIV OF TECH
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