Novel method for extracting baicalin from scutellaria baicalensis

A technology of baicalin and a new method, applied in the field of natural product separation, can solve the problems of complex process and low extraction rate, and achieve the effects of simple process, improved extraction rate, and increased exercise frequency and speed.

Inactive Publication Date: 2013-12-18
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the extraction rate of this method is low, the product purity is about 90%, and the process is complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Grind Scutellaria baicalensis, pass through a 50-mesh sieve, add 0.02% cellulase of Scutellaria baicalensis mass to 100g crushed scutellaria baicalensis, adjust the pH value to 6.0 with hydrochloric acid, extract in an ultrasonic extractor at 40°C for 80min, and the wave power is 300W; ultrasonic extraction After filtering to obtain the extract, the filter residue was repeatedly extracted twice, combined with the filtrate, added to the AB-8 macroporous adsorption resin column for adsorption, first washed the resin column with water until it had no sugar color, and then added 5 times of 60% ethanol to the filtrate to elute the active ingredients. Collect the eluent, concentrate under reduced pressure to obtain a concentrated solution; place the concentrated solution to crystallize, filter out to obtain crude crystals, recrystallize twice with ethanol, filter the crystals, and dry them to obtain high-purity baicalin with an extraction rate of 10.3 %, detected by HPLC, the ...

Embodiment 2

[0016] Crush Scutellaria baicalensis, pass through a 50-mesh sieve, add 0.03% cellulase of Scutellaria baicalensis mass to 100g crushed scutellaria baicalensis, adjust the pH value to 5.5 with hydrochloric acid, extract in an ultrasonic extractor at 50°C for 70min, and use a wave power of 400W; ultrasonic extraction After filtering to obtain the extract, the filter residue was repeatedly extracted twice, the combined filtrate was added to the ADS-7 macroporous adsorption resin column for adsorption, the resin column was first washed with water until it had no sugar color, and then 70% ethanol was added to the filtrate to elute the active ingredients. Collect the eluent, concentrate under reduced pressure to obtain a concentrated solution; place the concentrated solution to crystallize, filter out, and obtain crude crystals, recrystallize twice with ethanol, filter the crystals, and dry them to obtain high-purity baicalin with an extraction rate of 12.1 %, detected by HPLC, the ...

Embodiment 3

[0018] Crush Scutellaria baicalensis, pass through a 50-mesh sieve, add 0.05% cellulase of Scutellaria baicalensis mass to 100g of crushed scutellaria baicalensis, adjust the pH value to 5.0 with hydrochloric acid, extract in an ultrasonic extractor at 45°C for 60min, and use a wave power of 350W; ultrasonic extraction After filtering to obtain the extract, the filter residue was repeatedly extracted twice, and the filtrate was combined and added to a D-101 macroporous adsorption resin column for adsorption. The resin column was first washed with water until it had no sugar color, and then 50% ethanol 6 times the amount of the filtrate was added to elute the active ingredients. Collect the eluate, concentrate under reduced pressure to obtain a concentrated solution; place the concentrated solution to crystallize, filter out to obtain crude crystals, recrystallize twice with ethanol, filter the crystals, and dry them to obtain high-purity baicalin with an extraction rate of 10.9%...

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Abstract

The invention discloses a novel method for extracting baicalin from scutellaria baicalensis, and particularly relates to a method of combining enzyme-ultrasonic extraction with macroporous resin purification. The method comprises the following steps: adding cellulase which accounts for 0.02-0.05wt% of the scutellaria baicalensis after adding 50-70% ethanol to the crushed scutellaria baicalensis; adjusting the PH value to 5.0-6.0 by use of hydrochloric acid; putting the mixture into an ultrasonic extractor and extracting for 60-80 minutes; filtering to obtain an extracting solution after ultrasonic extraction; repeatedly extracting the filter residue twice, mixing filtrates, and adding the mixture to a macroporous absorbent resin column for adsorption; firstly, washing the resin column by water until no color of caramel appears, and then adding ethanol to elute effective components; collecting eluate, carrying out vacuum concentration, and standing a concentrated solution for crystallizing; filtering out rough crystal, recrystallizing by use of ethanol by twice, so as to obtain the high-purity baicalin. The method is free of toxicity, mild in reaction condition, high in extraction efficiency, short in extraction time, low in extraction cost and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of separation of natural products, and relates to a new method for extracting baicalin from Scutellaria baicalensis, in particular to a method combining enzyme-ultrasonic extraction and macroporous resin purification. Background technique [0002] Scutellaria baicalensis is the dry root of Scutellaria baicalensis plant of Labiatae. It is an important traditional Chinese medicine commonly used. It was first recorded in "Shen Nong's Herbal Classic" and has a long history of clinical application. Its main component, baicalin, is bitter in taste and cold in nature. It has the effects of purging excess fire, clearing heat and detoxifying, stopping bleeding and preventing miscarriage, lowering blood pressure, sedating, diuretic and choleretic, anti-inflammatory and anti-allergic. It is widely used clinically for pneumonia, hepatitis, chronic Bronchitis, high blood pressure, suppurative infection and threatened abortion and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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