Separation method for erythrodiol in pittosporum leaf crude extract and new application of erythrodiol

A technology of erythrodiol and crude extract, which is applied in the fields of chemistry and agriculture, can solve the problem of gentiana [Zhao Lei. Master's degree thesis of Yunnan University of Traditional Chinese Medicine, 2009], Sihemu [Ding Linlin. Tianjin Medical Sciences]. University Master's Degree Thesis, 2010], Leaf-leaved Pterocarpus [Tian Jun, Wu Fenge, Qiu Minghua, et al. Yunnan Botanical Research, 1995, 17(1): 108-110], Guangpi Papaya [Gao Huimei, Wu Lijun, et al. Chinese natural medicine and other issues

Inactive Publication Date: 2013-12-18
QINGDAO AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Although previously, it has been obtained from Gentiana gentiana [Zhao Lei. Master’s thesis of Yunnan University of Traditional Chinese Medicine, 2009], Sihemu [Ding Linlin. , et al. Yunnan Plant Research, 1995,17(1):108-110], papaya [Gao Hui, Wu Lijun, et al. China Natural Medicine, 2003, l(2):82-84] and Malan [Xu Wenqing, Gong Xiaojian, Zhou Xin, et al. Chinese Herbal Medicine, 2010,41(7):1056-1060] extracted and isolated from olives and other plants, and it has been reported that the compound has anti-tumor, anti-inflammation, anti-cancer cell proliferation and vasodilation, etc. Pharmacological activity [Yang Yanjun, Chen Huiyun, Lin Jiehong, et al. Journal of Guangzhou University of Traditional Chinese Medicine, 2007, 24(2): 158-161; Feng Weixiu. Foreign Medicine Traditional Chinese Medicine Volume, 2007, 24(2): 158-161], but not yet See reports on herbicidal activity

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  • Separation method for erythrodiol in pittosporum leaf crude extract and new application of erythrodiol
  • Separation method for erythrodiol in pittosporum leaf crude extract and new application of erythrodiol

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Embodiment 1

[0015] The extraction of embodiment 1 compound erythrodiol

[0016] Get the ethyl acetate extract 65g of Pittogonia leaf dry powder ethanol extract through silica gel column chromatography, use successively 20:1, 15:1, 10:1, 5:1, 3:1, 2:1, 1:1 and 0:1 volume ratio of petroleum ether and acetone mixed solvent and 50:1, 20:1, 10:1, 5:1 and 2:1 volume ratio of acetone and methanol mixed solvent for gradient elution to obtain A1~A10 a total of 10 cuts, the higher active A4 cuts are further separated by silica gel column chromatography with 6:1, 5:1, 4:1, 3:1, 2:1 and 1:1 volume ratio of sherwood oil and acetone mixed solvent, Obtain erythrodiol as white needle crystals.

[0017] The structure of the compound erythrodiol isolated above was identified, and the obtained spectral data are as follows:

[0018] ESI-MS spectrum display [M+H] + is 443;

[0019] 1 H-NMR (CDCl 3 , 500MHz) spectrogram shows δ: 5.19 ( 1H, t, J = 3.5 Hz, H-12), 3.56 (1H, d, J = 11.0 Hz, H-3), 3.20 (2H...

experiment example 2

[0021] Experimental example 2 The herbicidal activity of each fraction of the ethyl acetate phase and erythrodiol of the ethanol extract of Pittosporum leaf dry powder

[0022] 1) Experimental materials

[0023] lettuce

[0024] 2) Experimental method

[0025] Agar method: Soak the lettuce seeds in 0.2% sodium hypochlorite solution for 15 minutes, rinse them with tap water for several times and place them under running water to absorb water for about 3~5 hours, then wash them with distilled water for several times, and place them evenly on absorbent paper in a square plate with a cover Add distilled water until the seeds do not float, place in a fully intelligent artificial climate box (HP1000GS-B type, Wuhan Ruihua Instrument Equipment Co., Ltd.) to germinate, and transplant when the radicle (or seed root) reaches 3~5mm On the 0.5% agar gel mixed with extracts of each stage (100mL prepared in advance, and evenly distributed into 3 small beakers), the extracts of each extrac...

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Abstract

The invention relates to the fields of chemistry and agriculture, and in particular relates to a method for separating erythrodiol from an ethyl acetate extract of an ethanol extract of pittosporum leaf dry powder. The method comprises the following steps: performing silica gel column chromatography on the ethyl acetate extract; performing gradient elution by using petroleum ether and acetone, as well as acetone and methanol in sequence to obtain 10 fractions; and further performing gradient elution on the M4 fraction with higher activity by using the petroleum ether and the acetone to obtain the erythrodiol. The invention further provides a new weeding application of the erythrodiol.

Description

technical field [0001] The invention relates to the fields of chemistry and agriculture, and specifically provides a method for extracting erythrodiol in the crude extract of Pitotonia chinensis leaves and its application. Background technique [0002] Pittosporum ( Pittosporum tobira (Thunb.) Ait.) alias Pittogonia flower, mountain alum, Qilixiang, Baozhuxiang, is a plant of the genus Pittogonia in the Pittogonia family. Volatile aromatic compounds such as citral, ethyl acetoacetate, and butanone alcohol in its branches and leaves have a strong air purification function [Li Lingling. Master's Thesis of Northwest A&F University, 2007]; plants can also absorb heavy metal Zn and photochemical smog , is a good anti-pollution tree species[Liang Shuying, Xia Shangguang, Hu Haibo. Urban Environment and Urban Ecology, 2008,21(5):21-24; Zhu Zhiliang, Liang Dong, Zhang Ronghua, etc. Journal of Agricultural Environmental Science 2006,25 (3):690-693]. Its roots can expel wind and ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A01P13/00
Inventor 罗小勇王霞
Owner QINGDAO AGRI UNIV
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