Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Separation method for erythrodiol in pittosporum leaf crude extract and new application of erythrodiol

A technology of erythrodiol and crude extract, which is applied in the fields of chemistry and agriculture, can solve the problem of gentiana [Zhao Lei. Master's degree thesis of Yunnan University of Traditional Chinese Medicine, 2009], Sihemu [Ding Linlin. Tianjin Medical Sciences]. University Master's Degree Thesis, 2010], Leaf-leaved Pterocarpus [Tian Jun, Wu Fenge, Qiu Minghua, et al. Yunnan Botanical Research, 1995, 17(1): 108-110], Guangpi Papaya [Gao Huimei, Wu Lijun, et al. Chinese natural medicine and other issues

Inactive Publication Date: 2013-12-18
QINGDAO AGRI UNIV
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although previously, it has been obtained from Gentiana gentiana [Zhao Lei. Master’s thesis of Yunnan University of Traditional Chinese Medicine, 2009], Sihemu [Ding Linlin. , et al. Yunnan Plant Research, 1995,17(1):108-110], papaya [Gao Hui, Wu Lijun, et al. China Natural Medicine, 2003, l(2):82-84] and Malan [Xu Wenqing, Gong Xiaojian, Zhou Xin, et al. Chinese Herbal Medicine, 2010,41(7):1056-1060] extracted and isolated from olives and other plants, and it has been reported that the compound has anti-tumor, anti-inflammation, anti-cancer cell proliferation and vasodilation, etc. Pharmacological activity [Yang Yanjun, Chen Huiyun, Lin Jiehong, et al. Journal of Guangzhou University of Traditional Chinese Medicine, 2007, 24(2): 158-161; Feng Weixiu. Foreign Medicine Traditional Chinese Medicine Volume, 2007, 24(2): 158-161], but not yet See reports on herbicidal activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Separation method for erythrodiol in pittosporum leaf crude extract and new application of erythrodiol
  • Separation method for erythrodiol in pittosporum leaf crude extract and new application of erythrodiol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The extraction of embodiment 1 compound erythrodiol

[0016] Get the ethyl acetate extract 65g of Pittogonia leaf dry powder ethanol extract through silica gel column chromatography, use successively 20:1, 15:1, 10:1, 5:1, 3:1, 2:1, 1:1 and 0:1 volume ratio of petroleum ether and acetone mixed solvent and 50:1, 20:1, 10:1, 5:1 and 2:1 volume ratio of acetone and methanol mixed solvent for gradient elution to obtain A1~A10 a total of 10 cuts, the higher active A4 cuts are further separated by silica gel column chromatography with 6:1, 5:1, 4:1, 3:1, 2:1 and 1:1 volume ratio of sherwood oil and acetone mixed solvent, Obtain erythrodiol as white needle crystals.

[0017] The structure of the compound erythrodiol isolated above was identified, and the obtained spectral data are as follows:

[0018] ESI-MS spectrum display [M+H] + is 443;

[0019] 1 H-NMR (CDCl 3 , 500MHz) spectrogram shows δ: 5.19 ( 1H, t, J = 3.5 Hz, H-12), 3.56 (1H, d, J = 11.0 Hz, H-3), 3.20 (2H...

experiment example 2

[0021] Experimental example 2 The herbicidal activity of each fraction of the ethyl acetate phase and erythrodiol of the ethanol extract of Pittosporum leaf dry powder

[0022] 1) Experimental materials

[0023] lettuce

[0024] 2) Experimental method

[0025] Agar method: Soak the lettuce seeds in 0.2% sodium hypochlorite solution for 15 minutes, rinse them with tap water for several times and place them under running water to absorb water for about 3~5 hours, then wash them with distilled water for several times, and place them evenly on absorbent paper in a square plate with a cover Add distilled water until the seeds do not float, place in a fully intelligent artificial climate box (HP1000GS-B type, Wuhan Ruihua Instrument Equipment Co., Ltd.) to germinate, and transplant when the radicle (or seed root) reaches 3~5mm On the 0.5% agar gel mixed with extracts of each stage (100mL prepared in advance, and evenly distributed into 3 small beakers), the extracts of each extrac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the fields of chemistry and agriculture, and in particular relates to a method for separating erythrodiol from an ethyl acetate extract of an ethanol extract of pittosporum leaf dry powder. The method comprises the following steps: performing silica gel column chromatography on the ethyl acetate extract; performing gradient elution by using petroleum ether and acetone, as well as acetone and methanol in sequence to obtain 10 fractions; and further performing gradient elution on the M4 fraction with higher activity by using the petroleum ether and the acetone to obtain the erythrodiol. The invention further provides a new weeding application of the erythrodiol.

Description

technical field [0001] The invention relates to the fields of chemistry and agriculture, and specifically provides a method for extracting erythrodiol in the crude extract of Pitotonia chinensis leaves and its application. Background technique [0002] Pittosporum ( Pittosporum tobira (Thunb.) Ait.) alias Pittogonia flower, mountain alum, Qilixiang, Baozhuxiang, is a plant of the genus Pittogonia in the Pittogonia family. Volatile aromatic compounds such as citral, ethyl acetoacetate, and butanone alcohol in its branches and leaves have a strong air purification function [Li Lingling. Master's Thesis of Northwest A&F University, 2007]; plants can also absorb heavy metal Zn and photochemical smog , is a good anti-pollution tree species[Liang Shuying, Xia Shangguang, Hu Haibo. Urban Environment and Urban Ecology, 2008,21(5):21-24; Zhu Zhiliang, Liang Dong, Zhang Ronghua, etc. Journal of Agricultural Environmental Science 2006,25 (3):690-693]. Its roots can expel wind and ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A01P13/00
Inventor 罗小勇王霞
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com