High-activity tea pigment, and extraction method and application thereof
An extraction method and technology of tea pigment, applied in tea extraction, application, food preparation, etc., can solve problems such as excessive use of organic solvents, low extraction rate of theabrownin, and easy contamination by Aspergillus, achieving low overall cost and effective The effect of high content and less impurities
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[0040] Example 1
[0041] A method for extracting highly active tea pigments, including the following steps:
[0042] A. Cross-link 200g glucose oxidase to 100g Fe with an average particle size of 70nm 3 O 4 , Laid flat on the bottom of a PES plastic (PES is polyethersulfone resin) container with a diameter of 20cm and a height of 40cm, and add 200gCO 2 And 100g block Al 2 O 3 Form the enzyme mixture, seal it, and set aside;
[0043] B. Crush 1kg of green tea to 100 meshes, add 10 liters of water, heat to boiling, let stand and cool for 1 hour at room temperature (18-25°C) and filter to form tea soup;
[0044] C. According to the ratio of 1kg tea soup to 10g enzyme mixture, put the enzyme mixture into the tea soup and circulate heating in the order of 40℃ for 2 hours and 20℃ for 1 hour;
[0045] D. After 24 hours, put the magnet into the container, suck the upper solution and filter once;
[0046] E. Partially evaporate the water in the filtrate to obtain a crude tea pigment extract, and...
Example Embodiment
[0062] Example 2
[0063] On the basis of Example 1, the Fe in step A 3 O 4 Change the average particle size of the tea to 60nm, and crush the tea leaves in step B to 80 mesh.
[0064] The obtained extract was subjected to chemical composition analysis according to conventional methods, and the results are as follows:
[0065] ingredient Theafucins Theaflavins Thearubicin content(%) 83% 11% 1%
[0066] The above results are average values, and the unit is grams / 100 grams.
[0067] The highly active tea pigment extracted by the above method is applied to fruit juice beverages and dairy products, and the specific application method is as in Example 1.
Example Embodiment
[0068] Example 3
[0069] On the basis of Example 1, the Fe in step A 3 O 4 Change the average particle size of the tea to 50nm, and crush the tea leaves in step B to 110 mesh.
[0070] The obtained extract was subjected to chemical composition analysis according to conventional methods, and the results are as follows:
[0071] ingredient Theafucins Theaflavins Thearubicin content(%) 86% 15% 2%
[0072] The above results are average values, and the unit is grams / 100 grams.
[0073] The highly active tea pigment extracted by the above method is applied to fruit juice beverages and dairy products, and the specific application method is as in Example 1.
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