Method of producing bean pulp hydrolysate in enzyme-acid method
A hydrolyzate and soybean meal technology, applied in the field of bioengineering, can solve the problems of low yield, many intermediate products, incomplete hydrolysis, etc., and achieve the effect of high yield, low damage, and thorough hydrolysis
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Embodiment 1
[0029] (1) Crush the soybean meal, add 2.0 times of water, raise the temperature to 80°C, and keep it warm for 3 hours;
[0030] (2) Cool down to 40°C, adjust the pH to 5.0, add 0.2% papain, and hydrolyze at 40°C for 60 minutes;
[0031] (3) Add 0.4% special enzyme for soybean meal hydrolysis to incubate for 6 hours under the condition of raising the temperature to 55°C and adjusting the pH to 8.0;
[0032] (4) Cool down to 30°C and slowly add sulfuric acid with 0.2 times the amount of soybean meal dropwise under stirring;
[0033] (5) Heat up to 95°C, keep warm and acidify for 20 hours;
[0034] (6) Cool down to 28°C and filter to obtain the finished product. The amino nitrogen content of the finished product is 3.3g / 100ml, the total nitrogen content can reach 4.5g / 100ml, and the appearance of the product is golden yellow. At the same time, the yield is 2.4t / t, and the available nitrogen is 93%.
Embodiment 2
[0036] (1) Crush the soybean meal, add 2.5 times of water, raise the temperature to 85°C, and keep it warm for 5 hours;
[0037] (2) Cool down to 42°C, adjust the pH to 5.0, add 0.3% papain, and hydrolyze at 45°C for 60 minutes;
[0038] (3) Under the conditions of raising the temperature to 55°C and adjusting the pH to 8.0, add 0.3% special enzyme for hydrolysis of soybean meal for 6 hours;
[0039] (4) Cool down to 30°C and slowly add sulfuric acid with 0.2 times the amount of soybean meal dropwise under stirring;
[0040] (5) Heat up to 105°C, keep warm and acidify for 20 hours;
[0041] (6) Cool down to 25°C and filter to obtain the finished product. The amino nitrogen content of the finished product is 3.5g / 100m, the total nitrogen content can reach 4.8g / 100ml, and the appearance of the product is golden yellow. At the same time, the yield is 2.6t / t, and the available nitrogen is 95%. .
Embodiment 3
[0043] (1) Crush the soybean meal, add 3 times of water, raise the temperature to 90°C, and keep it warm for 4 hours;
[0044] (2) Cool down to 45°C, adjust the pH to 5.0, add 0.5% papain, and hydrolyze for 60 minutes at 45°C;
[0045] (3) Add 0.6% special enzyme for soybean meal hydrolysis to incubate for 6 hours under the condition of raising the temperature to 55°C and adjusting the pH to 8.0;
[0046] (4) Cool down to 30°C and slowly add sulfuric acid with 0.4 times the amount of soybean meal dropwise under stirring;
[0047] (5) Heat up to 100°C, keep warm and acidify for 18 hours;
[0048] (6) Cool down to 30°C and filter to obtain the finished product. The amino nitrogen content of the finished product is 3.4g / 100ml, the total nitrogen content can reach 4.7g / 100ml, and the appearance of the product is golden yellow. At the same time, the yield is 2.5t / t, and the available nitrogen is 94%. .
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