A kind of culture medium and fermentation method for erythromycin fermented by Streptomyces erythromycin

Abstract

The invention relates to a culture medium and a fermentation method for fermenting and producing erythromycin by Streptomyces erythromycin. The primary seed medium, secondary seed medium and fermentation medium for producing erythromycin by Streptomyces erythromycin are all Contains sucrose and corn steep liquor. In the present invention, medium-effect carbon and nitrogen sources such as corn steep liquor, urea and sucrose are used to replace the original slow-acting carbon and nitrogen sources, and corn steep liquor, urea and sucrose can be dissolved in water, thereby reducing the viscosity of fermentation broth, increasing dissolved oxygen, and enhancing its utilization speed , especially in the later stage of fermentation, it can be fully utilized, reducing the solid residues at the end of fermentation, thereby greatly reducing the amount of bacteria residue and the content of BOD and COD in wastewater, achieving energy saving, consumption reduction, pollution control at the source, and clean production good effect.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a culture medium and a fermentation method for producing erythromycin by fermentation of Streptomyces erythromycin. Background technique [0002] Erythromycin (Er) is a class of macrolide antibiotics, mainly including erythromycin A (ErA), erythromycin B (ErB) and erythromycin C (ErC). The antibacterial activity of B and C is the strongest, and the antibacterial activity of B and C is lower. In the prior art, in the process of fermenting and producing erythromycin with Streptomyces erythromycin as the fermentation strain, a large amount of late-acting soybean cake powder, starch, etc. are used in its seed medium, fermentation medium, and feeding medium. Carbon and nitrogen sources make the solid content of the culture medium greater than 6%, the solid content of insoluble carbon sources is greater than 3%, and the total solid content is greater than 9...

AI Technical Summary

Problems solved by technology

In the prior art, in the process of fermenting and producing erythromycin with Streptomyces erythromycin as the fermentation strain, a large amount of late-acting soybean cake powder, starch, etc. are used in its seed medium, fermentation medium, and feeding medium. Carbon and nitrogen sources make the solid content of the culture medium greater than 6%, the solid content of insoluble carbon sources is greater than 3%, and the total solid content is greater than 9%. The existence of these solid materials increases the difficulty of sterilization and the cost of raw materials, and increases the fermentation process. The viscosity of the liquid limits the oxygen supply during the fermentation process, and it cannot be fully utilized by the bacteria during the fermentation process, resulting in a solid content of more than 12% in the fermentation liquid at the end of the fermentation, which increases the consumption of raw materials, auxiliary facilities, and materials, and increases the fermentation rate. Difficulty and cost of filtration, purification and wastewater treatment in downstream liquid treatment