Olaquindox metabolite immunochromatographic test paper card and preparation method thereof
An immunochromatographic test paper and metabolite technology, which is applied in the field of olaquindox metabolite immunochromatographic test paper card and its preparation, can solve the problems of inability to operate on-site, high detection cost, complicated processing, etc., and achieve strong specificity and high sensitivity , the effect of simple operation
Inactive Publication Date: 2016-04-27
HENAN INST OF SCI & TECH
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Problems solved by technology
[0003] The residual detection methods of olaquindox and its metabolites mainly include thin-layer chromatography, high-performance liquid chromatography, mass spectrometry, high-performance liquid chromatography-mass spectrometry, etc. These methods are sensitive, accurate, specific, and have good resolution. Expensive instruments and equipment, complex sample pretreatment, tedious and time-consuming, high detection cost, cannot be operated on site, and requires professional and technical personnel; in contrast, ELISA has strong specificity, high sensitivity, easy operation, and low detection cost , suitable for the screening of batch samples, etc., and requires less equipment, easy to learn, and low cost, which can better meet the testing work of aquaculture, food processing enterprises, and drug residue monitoring departments in my country.
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[0021] Preparation of detection antigen
[0022] Accurately weigh 40mg of 3-methylquinoxaline-2-carboxylic acid (MQCA), 12mg of N-hydroxysuccinimide (NHS), 45mg of N,N-dicyclohexylcarbodiimide (DCC), dissolve Pour into 2ml of 1,4-dioxane solution, stir and react in the dark at 37°C for 12 hours, centrifuge to remove the precipitate, and call it A solution. Dissolve 20mg of OVA in 5ml of carbonate buffer solution (CBS, 0.05mol / L, pH9.6), called solution B. Add solution A to solution B dropwise under stirring condition, shake and react overnight at 4°C. After the reaction was completed, it was centrifuged, and the supernatant was dialyzed with PBS buffer solution for 6 days, and the prepared detection antigen MQCA-OVA was freeze-dried and stored. The detection antigen synthesis route is shown in the following reaction equation.
[0023]
[0024] Preparation method of specific monoclonal antibody against olaquindox metabolite
[0025] 1. Artificial antigen synthesis: the a...
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The invention discloses an olaquindox metabolite immunochromatography test paper card and a preparation method thereof and belongs to the technical field of veterinary drug residue immunochemistry fast detection. The test paper card comprises a plastic card shell and a test paper strip encapsulated in the plastic card shell. A sample adding hole and an observation window are formed in the plastic card shell. The bottom layer of the test paper strip is a support layer. A sample pad, a colloidal gold pad, a testing pad and a water absorption pad which are connected in a overlap manner are sequentially attached to the support layer from left to right. The test paper card is simple to operate, fast, accurate, high in flexibility, high in specificity, vivid and visualized in result display, and the like. In addition, the test paper card is capable of on-site monitoring and suitable for screening of a huge amount of samples.
Description
technical field [0001] The invention belongs to the technical field of immunochemical rapid detection of veterinary drug residues, and in particular relates to a olaquindox metabolite immunochromatographic test paper card and a preparation method thereof. Background technique [0002] Olaquindox (Olaquindox), also known as quinamide alcohol and fast Yuling, is a feed additive developed and synthesized by West German Bayer Pharmaceutical Factory in the early 1970s. Because of its broad-spectrum antibacterial effect and growth-promoting effect, it is widely used in Livestock and poultry breeding, antibacterial treatment and disease prevention and control of aquatic animals. However, a large number of biological toxicity tests show that: excessive olaquindox will remain in the animal body, which has obvious cumulative toxicity, carcinogenicity, mutagenesis and DNA damage and other toxic side effects. In view of the harm to human health, the EC2788 / 98 resolution of the European...
Claims
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IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 张慧辉姜金庆王自良李广领杨雪峰范国英杨理赵旭娜刘长忠
Owner HENAN INST OF SCI & TECH