Unlock instant, AI-driven research and patent intelligence for your innovation.

Gastric cancer-targeting quantum dots, preparation method and application thereof

A technology of quantum dots and gastric cancer, which is applied in the field of nano-biological materials, can solve problems such as gastric cancer, and achieve good effects, small attenuation, and clear fluorescence imaging

Inactive Publication Date: 2016-08-10
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The emission spectrum of organic dyes is usually between 450-550. In this range, biomedical specimens such as collagen, porphyrin, flavin, etc. usually have a strong autofluorescence background, so the labeled fluorescence is usually dominated by strong tissue autofluorescence. Submerged, there are many limitations in the application of bioluminescent imaging
Some studies have begun to pay attention to the distribution of near-infrared quantum dots in animals, and observe the migration in the circulatory system of animals and the distribution in various organs after injecting pure quantum dots. Reports on the tracking and imaging of tumor cells, especially in the field of gastric cancer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gastric cancer-targeting quantum dots, preparation method and application thereof
  • Gastric cancer-targeting quantum dots, preparation method and application thereof
  • Gastric cancer-targeting quantum dots, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of near-infrared CdTe quantum dots

[0038] First, prepare NaHTe solution: dissolve 100mg NaBH4 in 20ml deionized water, and pass a steady nitrogen flow for 30 minutes in an ice bath to remove oxygen in the solution, then quickly add 127mg tellurium powder, and continue to pass nitrogen to make a lavender NaHTe solution. Next, make CdCl2-MPA solution: add 366.6mg (2mmol) CdCl2 to 100ml deionized water, then add 382.1mg (3.6mmol) MPA, and adjust the pH of the solution to 9 with 2mol / L NaOH. The final step is to make CdTe quantum dots: mix 1ml NaHTe solution with 20ml CdCl2-MPA solution and stir quickly, then add 9mL of the former body solution into the reaction kettle, put it in an oven at 185°C for a period of time, and wash the crude product with ethanol precipitation 3 times, put it into a vacuum oven at 40°C and dry it for later use.

[0039] Preparation of gastric cancer cell targeting quantum dots

[0040] Mix 13.5 μl EDC, 13.5 μl NHS and 50 μl quantu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the field of nano biological materials, and relates to stomach cancer targeting quantum dots, and a preparation method and an application thereof in tumor detection. CdTe near-infrared quantum dots are adopted as an imaging material, are combined with a stomach cancer monoclonal antibody CC49, and are made into an immune fluorescent probe capable of specific binding with stomach cancer cells and fluorescent imaging. Quantum dots which have good biocompatibility in a biological body and can run with a circulatory system are utilized to prepare the targeting quantum dots for immune fluorescent imaging of the stomach cancer cells, combination of a tumor metastasis part and tumor cells in a stomach cancer animal model is further detected, fluorescence is emitted after exciting to make the tumor cells image, so as to achieve the purpose of tumor detection. The targeting quantum dots based on the biological body have smaller toxicity and have a potential clinical application value.

Description

technical field [0001] The invention belongs to the field of nano-biological materials, and relates to a gastric cancer-targeting quantum dot, a preparation method thereof, and an application in tumor detection, in particular to a gastric cancer immunofluorescence probe, a preparation method thereof, and an application in fluorescence imaging of gastric cancer cells. Background technique [0002] According to reports, the incidence of gastric cancer in the world has decreased to a certain extent in the past 50 years, but it is still a disease that seriously endangers human health. Among various cancers, the incidence rate of gastric cancer ranks fourth, even as high as 0.080% in Japan, South Korea, North Korea, China and other Asian countries; its mortality rate ranks second, second only to lung cancer, reaching 85%. At present, surgical treatment is an important means of clinical treatment of gastric cancer. However, in clinical practice, the dissection of tumor metastatic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/533G01N21/64G01N33/574
CPCG01N33/57446G01N33/588G01N2800/7028
Inventor 孙鹏张云鹏杨武利张旭瑞
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com