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Mussel mucoprotein liquid product as well as preparation method and application thereof

A technology for mussel mucin and liquid products, which is applied in the preparation methods of peptides, biochemical equipment and methods, applications, etc., to achieve the effects of enhancing stability, wide application and increasing protein molecular weight

Inactive Publication Date: 2014-01-22
高敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a preparation method of mussel mucin liquid product, mussel mucin liquid product and application thereof, and mussel mucin is used as raw material to form new mussel mucin by means of modification, addition or cross-linking Liquid product, to solve the defects caused by the direct use of mussel mucin solution state in the prior art

Method used

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  • Mussel mucoprotein liquid product as well as preparation method and application thereof
  • Mussel mucoprotein liquid product as well as preparation method and application thereof
  • Mussel mucoprotein liquid product as well as preparation method and application thereof

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preparation example Construction

[0045] Specifically, the present invention proposes a method for preparing mussel mucin liquid product, comprising the following steps (such as figure 1 ):

[0046] Step 1: obtaining or preparing liquid mussel mucin;

[0047] Step 2: adding a modifier to the liquid mussel mucin to chemically modify the mussel mucin.

[0048] Wherein, after the step 2, it may also include: step 3: adding a cross-linking agent to the modified mussel mucin, and using the cross-linking agent to make the mussel mucin form an intramolecular cross-link (such as figure 2 ).

[0049] Moreover, after the step 2, it may also include: step 3': adding additives and a cross-linking agent to the modified mussel mucin at the same time, and making the mussel mucin and the mussel mucin through the cross-linking agent Additives form intermolecular crosslinks (eg image 3 ).

[0050] After the step 2, it also includes: step 3", adding additives to the modified mussel mucin, so that an intermolecular cross-l...

Embodiment 1

[0080] Take 1 ml of mussel mucin solution with a concentration of 0.01 mg / mL, and pass oxygen (modification) for 30 minutes at room temperature. The modified protein is used for BEK-21 cell culture, and the results are observed 6 hours after cell inoculation. Such as Figure 4 , Figure 5 As shown, compared with the results without adding mussel mucin, the number of cells attached to the culture plate added with the modified mussel mucin was more, which proved that the addition of mussel mucin promoted the adhesion performance of the cells.

[0081] The protein solution obtained by the same preparation method was used in the animal bone defect experiment, that is, after mixing mussel mucin and bone powder, it was added to the bone defect of the rat hind leg, and the bone powder without mussel mucin was used as a control, and observed after six weeks Bone growth in the defect. It can be seen that compared with the bone powder without protein, the bone powder added with mussel...

Embodiment 2

[0083] Take 1 ml of mussel mucin solution with a concentration of 5 mg / mL, add 100 μL of iodine solution with a concentration of 0.01% (mass percentage) at room temperature, and mix the mussel mucin and iodine solution thoroughly before use. The modified protein was used for HEK cell culture, and the results were observed 2 hours after cell inoculation.

[0084] Such as Figure 6 , Figure 7 As shown, compared with the results without adding mussel mucin, the number of cells attached to the culture plate added with the modified mussel mucin was more, which proved that the addition of mussel mucin promoted the adhesion performance of the cells.

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Abstract

The invention discloses a mussel mucoprotein liquid product as well as a preparation method and an application thereof. One or more of a modifier, an additive and a crosslinker are added into mussel mucoprotein to form a new product which can serve as a wound repair product, a wound protection product, a medical biological bonder product, a medical coating product, an industrial coating product, a biochemical reagent product or the like. According to the mussel mucoprotein liquid product and the preparation method of the mussel mucoprotein liquid product, the modifier, the additive and the crosslinker are added into the mussel mucoprotein, so that the stability of a protein structure is enhanced, the protein molecular weight is increased, and the mussel mucoprotein liquid product is widely applied in various fields.

Description

technical field [0001] The invention relates to mussel mucin liquid products, in particular to preparing wound repair products, wound protection products, medical biological adhesive products, medical coating products, industrial coating products, and biochemical reagent products using mussel mucin as raw materials and other liquid products. Background technique [0002] Mussel adhesive protein (MAP), also known as Mytilus edulis foot protein (Mefp), is a special protein secreted from the marine shellfish Mytilus purpurea. Blue mussels are usually attached to the reefs along the coast or the bottom of ships in groups, and have the ability to withstand the impact of waves in the offshore. In fact, blue mussels can be extremely firmly attached to almost any substrate, such as metal, wood, glass, etc. The main reason why mussels have the above characteristics is that a special protein can be produced and stored in the silk gland, which is released to solid surfaces such as ro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L24/10A61L27/22A61L27/34A61L31/04A61L31/10C07K1/107C12N5/00
Inventor 高敏
Owner 高敏
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