Penicillium oxalicum bam-1 and its separation and purification method and application

A Penicillium oxalate, application method technology, applied in the field of microorganisms, can solve the problems of no ecology, environmental protection and the like

Active Publication Date: 2015-08-05
RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no ecological and environmentally friendly treatment method for the large amount of organic mulch left in the mulch of thunder bamboo forest. Bamboo farmers often adopt the method of deep plowing forest land and adding soil to reduce the negative impact caused by the retention of organic mulch. However, it still cannot solve the practical problem that organic mulch is difficult to effectively decompose in the short term.

Method used

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  • Penicillium oxalicum bam-1 and its separation and purification method and application
  • Penicillium oxalicum bam-1 and its separation and purification method and application
  • Penicillium oxalicum bam-1 and its separation and purification method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Isolation, activation and cultivation of bacterial strains

[0028] The strain was isolated from the organic mulch and the soil covering the Phyllostachys praecox forest that had been piled up for a long time in Gaoyun Village, Taihuyuan Town, Lin'an City, Zhejiang Province. It was a pure strain obtained through enrichment culture of samples, primary screening, and secondary screening.

[0029] Enrichment culture of samples: Weigh 10.0 g of soil and decayed organic mulch samples, add them into a 250 ml Erlenmeyer flask filled with 90 ml of sterile water and glass beads, 180 r min -1 Shake for 30 min to fully disperse the sample, use a sterile pipette to take 5 ml of the suspension and inoculate it into 45 ml of enrichment medium, at 28 °C, 120 r min -1 Cultured with shaking for 2 days. Then take the culture solution and inoculate it into fresh sterile enrichment medium, and culture repeatedly for 3 times.

[0030] The enrichment medium adopts the impro...

Embodiment 2

[0039] Example 2: Strain filter paper enzyme activity

[0040] Penicillium oxalicum ( Penicillium oxalicum) BAM-1, Trichoderma viride, and Trichoderma harzianum were inoculated in Hutchison liquid medium (formula: 1 cm×6 cm Xinhua quantitative filter paper, KH 2 PO 4 1.05g, NaCl 0.11g, MgSO 4 ·7H 2 O 0.29 g, NaNO 3 2.51 g, FeCl 3 0.01g, CaCl 2 0.10g, 1000 ml water, pH 6.05), 120 r·min at 28°C -1 Shake flask shaking culture, see the results figure 1 , it was found that the filter paper enzyme activities of the three strains first increased and then decreased with the prolongation of culture time, and the enzyme activities were the highest at 5 days of culture, which were 9.83 U, 3.93 U, and 4.64 U, respectively, and Penicillium oxalicum ( Penicillium oxalicum) The enzyme activity of BAM-1 filter paper is more than 2 times that of Trichoderma viride and Trichoderma harzianum.

Embodiment 3

[0041] Example 3: Strain Enzyme Production Medium Optimization

[0042] Based on the single factor test, the L 9 (3 4 ) Orthogonal experimental design, for carbon sources containing different concentrations (rice straw 10 g·L -1 , 15 g·L -1 , 25g L -1 ), nitrogen source (beef extract 1.5 g L -1 , 2.0 g L -1 , 2.5 g L -1 ) and inorganic salts (FeCl 3 0.01 g·L -1 , 0.015 g·L -1 , 0.02 g g L -1 and KH 2 PO 4 1.0g L -1 , 1.5 g·L -1 , 2.0 g L -1 ) Hutchinson liquid medium was optimized, see Table 1. The optimal combination of medium factors for enzyme production by strains is: rice straw 25 g·L -1 , beef extract 2.5 g·L -1 , FeCl 3 0.01 g·L -1 、KH 2 PO 4 1.5 g·L -1 .

[0043] Table 1L 9 (34 ) Orthogonal Experimental Design and Results

[0044]

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Abstract

The invention provides a bacterial strain Penicillium oxalicum (Penicillium oxalicum) BAM-1. The strain is a pure strain isolated from the organic material of the forest land covering the soil of Leizhu forest and the organic mulch that has been stacked for a long time. The sequence of the strain is highly homologous (99.3% to the 26S rRNA gene sequence of Penicillium oxalicum) ). The invention provides the cultivation, enzyme production and fermentation conditions of the bacterial strain and the environmental conditions applied in bamboo forests. The strain can effectively decompose straw and chaff, which are high in cellulose content, and accelerate the rapid ecological decomposition of the remaining organic mulch in the forest, especially in the sustainable management of bamboo forests, which has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Penicillium oxalicum BAM-1, a separation and purification method and application thereof. Background technique [0002] Phyllostachys praecox is an excellent bamboo species for bamboo shoots in my country. It has delicious taste, high yield and good benefits. It has been cultivated on a large scale in Zhejiang, Jiangxi and other places in southern my country. At present, the area of ​​Phyllostachys praecox forest in my country has reached more than 200,000 ha -2 . Bamboo shoots mainly planted with thunder bamboo species have become a pillar industry for regional rural economic and social development and the main source of economic income for farmers' families. Since the early 1990s, many bamboo production areas for bamboo shoots have widely promoted the application of organic materials (mainly rice bran and rice straw) forest land mulching technology, using ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C09K17/00C12R1/80C09K101/00
Inventor 郭子武陈双林李迎春杨清平
Owner RES INST OF SUBTROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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