Method for screening radiation-damaged biomarkers at early stage of rat and application

A technology for biomarkers and radiation damage, applied in the field of radiation life sciences, can solve problems such as cumbersome determination process and complicated estimation methods

Inactive Publication Date: 2014-01-22
SUZHOU UNIV
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Problems solved by technology

However, these biological dose estimation methods are generally more complicated, the measuremen

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  • Method for screening radiation-damaged biomarkers at early stage of rat and application
  • Method for screening radiation-damaged biomarkers at early stage of rat and application
  • Method for screening radiation-damaged biomarkers at early stage of rat and application

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Embodiment

[0037] 1. Materials and methods

[0038] (1) Gas chromatography and mass spectrometry conditions

[0039] Gas chromatography spectrometer 7890A-5975C GC-MS system (Agilent); column: DB-5MS capillary column (0.25 μm, 0.25 μm×30 m, Agilent Technologies, USA); carrier gas: helium; carrier gas flow rate 1.1ml / min; splitless injection of 1 μL derivatized sample; the temperature of the injection port was 250 °C; the temperature program mode was adopted, and the initial column temperature was 80 °C and maintained for 5 min; then the temperature was raised from 80 °C to 210 ℃, 5 ℃ / min heating up to 250 ℃, 10 ℃ / min heating up to 300 ℃ and keeping for 8 min, solvent cutting time is 5 min.

[0040] Mass spectrometry conditions: quadrupole mass spectrometer; electron bombardment ion source (EI); ionization voltage is 70eV; ion source temperature and interface temperature are 230°C and 280°C, respectively; detector voltage is 1.2kV; mass scanning range is 30- 600 m / z .

[0041] (2) Re...

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Abstract

The invention discloses a method for screening radiation-damaged biomarkers at the early stage of a rat and an application. The method comprises the following steps of firstly adopting the rat as an animal model, utilizing radiation metabonomics method, adopting a gas chromatography-mass spectrum (GC-MS) combined technology to research the influence of acute ionizing radiation on the change of metabolites of the rat plasma; then utilizing a genetic algorithm (GA) and univariate statistics to analyze and find out potential radiation-damaged biomarkers; finally using the biomarkers for early-stage injury classification of radiation damage by combination with a multivariate statistic analysis method--non-linear iterative partial least square method (KPLS). The invention has the advantages that by adoption of the technical scheme, injury classification can be carried out on a great amount of radiation-exposing crowds in ionizing radiation accidents in a short time so as to have an important significance in early-stage diagnosis and prevention of dysfunction or death of organs; the biomarkers are used for studying the classification of radiation-damaged injuries, so that the limitation of single index for estimation of radiation-damaged dosage is overcome.

Description

technical field [0001] The invention belongs to the field of radiobiology, and specifically relates to a method and application for screening early radiation injury biomarkers in rats. Background technique [0002] With the development of nuclear energy, nuclear power and nuclear technology, the number of people engaged in radioactive work is increasing, and occupational overdose exposure and accidental radiation injury are more and more likely to occur. At present, there are physical methods and biological dosimetry methods for the determination of individual ionizing radiation exposure dose. After a radiation accident or nuclear terrorism incident, when the general exposed person does not wear a personal dosimeter or the exposure dose exceeds the rated range of the dosimeter, the biological dosimeter can be used to estimate the radiation dose of the exposed person to provide a basis for clinically determining the treatment plan. In recent years, the ionizing radiation b...

Claims

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Application Information

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IPC IPC(8): G01T1/02G01N33/48G01N30/88
Inventor 王畅张圆圆郭景德李超武建芳周娴
Owner SUZHOU UNIV
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