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Potential fingerprint imaging method based on electrochemiluminescence immunoassay

A luminescence immune and electrochemical technology, applied in the fields of analytical chemistry and fingerprint detection, can solve the problems of unfingerprint detection and imaging, and achieve the effects of rapid detection, convenient rapid real-time detection, and simple method.

Active Publication Date: 2015-02-25
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are currently no reports on the detection and imaging of fingerprints using electrochemiluminescence immunoassays

Method used

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  • Potential fingerprint imaging method based on electrochemiluminescence immunoassay
  • Potential fingerprint imaging method based on electrochemiluminescence immunoassay
  • Potential fingerprint imaging method based on electrochemiluminescence immunoassay

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] (1) Dip a small amount of fresh blood on the finger, and press a blood fingerprint on the gold-plated polystyrene sheet;

[0069] (2) Immunolabeling: draw a circle around the fingerprint with an immunohistochemical pen to avoid the diffusion of the antibody solution, and dissolve 100 μL of 0.1 mg / mL goat anti-human IgG (solvents for dissolving antibodies contain 2% BSA, 0.1% Tween -20 PBS) was dropped on the fingerprint, incubated at room temperature for 30min, rinsed with PBS-T to wash away unbound antibody, dried the surface with argon, and then 100μL 0.05mg / mL rabbit anti-goat IgG / HRP was dropped On the fingerprints, incubate at room temperature for 30 min, rinse with PBS-T and dry with argon.

[0070] (3) Electrochemiluminescence imaging: Place the above-mentioned gold-plated polystyrene sheet (working electrode), counter electrode and reference electrode in the electrochemical reaction cell, add the electrochemiluminescence reaction solution, power on the electroch...

Embodiment 2

[0073] 1. Three-step method

[0074] (1) Press a sweaty fingerprint on the gold-plated polystyrene sheet;

[0075] (2) Immunolabeling: draw a circle around the fingerprint with an immunohistochemical pen to avoid the diffusion of the antibody solution, drop 100 μL 0.1 mg / mL epidermal growth factor antibody on the fingerprint, incubate at room temperature for 30 minutes, rinse with PBS-T Wash off unbound antibodies, dry the surface with argon, then drop 100 μL 0.01 mg / mL goat anti-rabbit IgG / biotin on the fingerprint, incubate at room temperature for 30 min, rinse with PBS-T and dry with argon, Then 100 μL of 5 μg / mL streptavidin / HRP was dropped on the fingerprint, incubated at room temperature for 30 min, rinsed with PBS-T and dried with argon.

[0076] (3) Electrochemiluminescence imaging: Place the above-mentioned gold-plated polystyrene sheet (working electrode), counter electrode and reference electrode in the electrochemical reaction cell, add the electrochemiluminescenc...

Embodiment 3

[0084] 1. Three-step method

[0085] (1) Press a sweaty fingerprint on the gold-plated polystyrene sheet;

[0086] (2) Immunolabeling: draw a circle around the fingerprint with an immunohistochemical pen to avoid the diffusion of the antibody solution, drop 100 μL 0.1 mg / mL lysozyme antibody on the fingerprint, incubate at room temperature for 30 minutes, rinse with PBS-T to wash To remove unbound antibodies, dry the surface with argon gas, then drop 100 μL 0.01 mg / mL goat anti-rabbit IgG / biotin on the fingerprint, incubate at room temperature for 30 minutes, rinse with PBS-T and dry it with argon gas, then 100 μL of 5 μg / mL streptavidin / HRP was dropped on the fingerprint, incubated at room temperature for 30 min, rinsed with PBS-T and dried with argon.

[0087] (3) Electrochemiluminescence imaging: Place the above-mentioned gold-plated polystyrene sheet (working electrode), counter electrode and reference electrode in the electrochemical reaction cell, add the electrochemilu...

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Abstract

The invention discloses a potential fingerprint imaging method based on an electrochemiluminescence immunoassay. The method includes pressing a fingerprint on an electrode base and obtaining a fingerprint sample; adding antibody solution of metabolites of a target to be detected to the fingerprint of the fingerprint sample to perform incubation, and washing through buffer solution after incubation is completed; drying the fingerprint sample, then adding a secondary antibody marked by horseradish peroxidase to the fingerprint to perform incubation; or drying the fingerprint sample, then adding a secondary antibody marked by biotin to the fingerprint, performing incubation for a period, washing and drying the fingerprint sample, and adding horseradish peroxidase marked by streptavidin to perform incubation; after the incubation, using the buffer solution to wash the fingerprint sample, preparing and obtaining an operating electrode after drying, and then acquiring a fingerprint image through an electrochemiluminescence imaging system. According to the method, fingerprint images are acquired, and meanwhile, specificity sensitive detection of potential fingerprint target metabolites can be implemented.

Description

technical field [0001] The invention belongs to the technical fields of analytical chemistry and fingerprint detection, and in particular relates to a latent fingerprint imaging method based on electrochemiluminescence immunoassay. Background technique [0002] Latent fingerprints are fingerprint lines formed by the transfer of natural body secretions (such as sweat), which are difficult to detect visually and are the most common fingerprints at crime scenes. According to incomplete statistics from various places in my country, among the criminal cases solved by the police, more than 70% are solved by using fingerprints, and the proportion is increasing year by year. Under normal circumstances, fingerprints are the most direct physical evidence left by criminals at the scene. Therefore, research on the display technology of latent fingerprints is an important link to improve the collection rate and utilization rate of fingerprint physical evidence. [0003] The development ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/117G01N33/535G01N21/76
Inventor 苏彬许林茹
Owner ZHEJIANG UNIV
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