Complex microbial inoculant for straw compost maturity and european corn borer prevention, as well as preparation and application of complex microbial inoculant

A compound bacterial agent and straw decomposing technology, applied in the directions of application, botanical equipment and methods, microorganism-based methods, etc., can solve the problems of cumbersome use methods, reduce the incidence rate, improve the aggregate structure, and accelerate the straw decomposing effect.

Inactive Publication Date: 2014-01-29
辽宁省微生物科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adding straw decomposing agent and additives during the process of straw returning to the field is a better way to solve this problem, but the method of use is cumbersome, and it is urgent to find a product that is easy to use and easy to accept by farmers

Method used

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  • Complex microbial inoculant for straw compost maturity and european corn borer prevention, as well as preparation and application of complex microbial inoculant

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] Composition and preparation of mixed strains:

[0026] 1. Composition and preparation of compound bacterial agent:

[0027] 1. Composition and preparation of liquid bacteria:

[0028] The purified and rejuvenated Trichoderma harzianum ( Trichoderma harzianum Rifai ), Bacillus licheniformis ( Bacillus licheniformis (Weigmann) Chaester ), Beauveria bassiana ( Beauveria bassiana )

[0029] The test tube slant culture medium is respectively inoculated in the corresponding liquid culture medium, and liquid strains are respectively made into liquid strains through liquid fermentation;

[0030] The weight composition of the Trichoderma harzianum culture medium: corn flour 2.0g, yeast extract 1.0g, potassium dihydrogen phosphate 0.05g, magnesium sulfate 0.05g, calcium chloride 0.01g, water 100ml, pH natural;

[0031] The weight composition of Beauveria bassiana culture medium: glucose 4.0g, peptone 1.0g, yeast extract powder

[0032] 1.0g, 100ml water, natural pH;

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Abstract

The invention discloses a complex microbial inoculant for straw compost maturity and european corn borer prevention, as well as preparation and application of the complex microbial inoculant. The complex microbial inoculant comprises a mixed strain and an additive, wherein the mixed strain comprises trichoderma harzianum, bacillus licheniformis and beauveria bassiana, and is prepared by the following steps: inoculating three bacterial strain test tube slant culture media subjected to purification and rejuvenation to corresponding fluid nutrient media respectively, performing fermentation to obtain liquid strains, inoculating the three fermented fluid strains into a solid culture medium, culturing for 48-72h at the temperature being 28-30 DEG C, dying, and crushing; the additive comprises glucose, superphosphate and humic acid; the mixed strain and the additive are mixed according to a mass ratio being (2.0-8.0):1.0 so as to obtain the complex microbial inoculant. The mixed strain and the additive are mixed so as to play a relatively good synergy role; the complex microbial inoculant has the capabilities of increasing the speed of straw compost maturity, killing european corn borer larvae and pupae, reducing the incidence of european corn borer, reducing the harm, improving granhle soil structures, increasing the fertilizer utilization rate and playing a yield increasing role.

Description

technical field [0001] The invention relates to a composite bacterial agent in the technical field of microbes, in particular to a composite bacterial agent capable of preventing and controlling corn borer and its preparation and application method, which is used for returning straw to the field and accelerating straw maturity. Background technique [0002] my country is a large agricultural country with a large quantity and variety of crop straws and abundant straw resources. During the development of modern agriculture, agricultural producers have gradually become indifferent to the recycling of organic waste, and a large amount of straw has been burned, which not only causes waste of resources, but also brings adverse effects on the ecological environment. According to statistics, the theoretical resource of crop straws in China is 820 million tons, which means that the utilization potential of straw resources in our country is huge. [0003] After the straw is returned ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05G3/02A01C21/00A01N63/04A01N63/00A01P7/04C12R1/885C12R1/10C12R1/645C05G3/60
Inventor 李莉池景良吴红艳王志学冯健于淼方新李鑫宗玉丽孙玉禄
Owner 辽宁省微生物科学研究院
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