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Method for preparing salt-tolerant microbial agent

A technology of microbial inoculum and microbial flora, applied in protozoa, chemical instruments and methods, biological water/sewage treatment, etc., can solve the problems of low treatment efficiency, poor resistance to salinity shock, and low salt tolerance.

Inactive Publication Date: 2014-01-29
BLUESTAR LEHIGH ENG INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The problem to be solved by the present invention is that the traditional biochemical method for treating high-salt wastewater has low salt tolerance, poor resistance to salinity impact, and low treatment efficiency; Changeable problems, providing a new method for preparing salt-tolerant microbial bacterial agents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, a method for preparing a salt-tolerant microbial bacterial agent, the steps are: in an open culture device, directly test the salt-tolerant microorganisms in a high-salt environment sample under the condition that the total salt mass concentration is 1-30%. Bacteria are mixed and cultivated, and the obtained culture solution is the salt-tolerant microbial agent; during the cultivation process, appropriate amount of organic carbon source, nutrient elements and trace elements are added according to the conventional cultivation method, the pH is controlled at 5.0-9.5, the temperature, dissolved oxygen, The total salt concentration remained stable. The prepared salt-tolerant microbial bacterial agent can be used for purification treatment of high-salt wastewater with a total salt concentration of 1-30%.

Embodiment 2

[0045] Example 2, in the preparation method described in Example 1: the open culture device is always in communication with the air during the culture process.

Embodiment 3

[0046] Example 3, in the preparation method described in Example 1 or 2: the sources of the high-salt environment samples are but not limited to: oceans, dead seas, salt lakes, salt pans, saline-alkali lands, salt mines, salt wells, sun-dried salt fields, pickled products, high Saline wastewater, soil.

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PUM

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Abstract

The invention discloses a method for preparing a salt-tolerant microbial agent. The method comprises the following steps of in an open culture device, directly carrying out mixed culture on a salt-tolerant microbial flora in a high-salt environment sample under the condition that total salt concentration is 1%-30%; and adding an organic carbon source, nutrient elements and trace elements according to the needs in a culture process, controlling and keeping proper pH, temperature and dissolved oxygen relatively stable. The salt-tolerant microbial agent prepared by adopting the method has good salt tolerance and salinity impact resistance, can degrade organic substances in water without being restrained by high-concentration inorganic salt under the condition that total salt concentration is 1%-30%, and can be directly used for purifying high-salt-content wastewater with salt concentration of 1%-30%. The method disclosed by the invention is simple to operate, short in implementation period and capable of quickly carrying out industrial application.

Description

technical field [0001] The invention belongs to the technical field of environmental protection microorganisms and sewage treatment, and in particular relates to a method for preparing a salt-tolerant microbial bacterial agent. Background technique [0002] High-salt wastewater refers to wastewater with a mass fraction of inorganic salts greater than 1%. Its sources are complex and diverse, and its direct discharge will cause great harm to the ecological environment and human health. High-salt wastewater has complex components and is one of the refractory wastewaters. The inorganic salts in it can inhibit or poison the growth of common microorganisms, which severely limits the application of biological treatment technology in high-salt wastewater. However, physical and chemical methods including evaporation, membrane separation, ion exchange, advanced oxidation, electrolysis, etc. not only do not treat pollutants thoroughly, but also easily cause secondary pollution; and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/10C02F3/34
Inventor 徐军王开春董自斌李学字云干赵焰杨志林刘志奎
Owner BLUESTAR LEHIGH ENG INST CO LTD
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