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Method for collecting microalgae through flocculation of protamine

A protamine and flocculation technology, which is applied in the field of collecting microalgae by using protamine flocculation, can solve the problems of carcinogenicity, high cost, teratogenicity and the like, and achieve the effects of improving flocculation efficiency, avoiding light and saving costs.

Inactive Publication Date: 2014-02-05
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic flocculants are cheap, but they will have adverse effects on human health and the ecological environment; although organic polymer flocculants are used in a small amount, have strong flocculation ability, and the flocs are easy to separate, but the residual monomers of such polymers have "three "causative" effect (teratogenic, carcinogenic, mutagenic); microbial flocculant is non-polluting to the environment, easy to use and safe, but the cost is high, and it is limited to the laboratory stage, so its application range is limited; protamine as flocculation Because there is no secondary pollution, it is easy to use, the flocculation effect is good, and the application prospect is attractive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Dissolve salmon protamine in water, prepare a protamine solution with a concentration of 0.5 g / L, and adjust the pH of the protamine solution to about 7.0. Add 2mL protamine solution to 40mL Salina salina, add 2mL distilled water to the control group, keep it in the dark for 2 hours, count the upper liquid with a hemocytometer, and the algae cell sedimentation degree reaches 92.2%

Embodiment 2

[0018] Example 2: Dissolving carp protamine in water, preparing a protamine solution with a concentration of 0.5 g / L, and adjusting the pH of the protamine solution to about 7.0. Add 2mL protamine solution to 40mL flat algae, add 2mL distilled water to the control group, keep it in the dark for 1h, count the upper liquid with a hemocytometer, and the algae cell sedimentation degree reaches 76.8%

Embodiment 3

[0019] Example 3: Add acetic acid to a salmon protamine solution with a pH of 7.0 and a concentration of 0.5 g / L to make the concentration of acetic acid 0.2%. Add 0.5mL protamine solution to 40mL Salina salina, add 0.5mL distilled water to the control group, keep it in the dark for 2 hours, count the upper liquid with a hemocytometer, and the algae cell sedimentation degree reaches 90.4%

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PUM

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Abstract

The invention discloses a method for collecting microalgae through flocculation of protamine. The method is simple and efficient, the flocculation rate can reach about 75-98% by using 5-40mg / L of protamine, after precipitation, cells of the algae still have better vitality and keep moving, and the algae can be transferred to a fresh liquid medium to be cultivated continuously. After flocculation, the nutrient solution of the upper layer is cultivated again, the growing condition of the microalgae is good, and the recultivated microalgae also can have good flocculation effect after being flocculated by protamine and has no flocculation inhibitive ability. The microalgae which is flocculated by protamine has good sedimentation effect, does not pollute the environment, is environment-friendly, effectively recycles the cultivation water body to the greatest degree, and has good practicability.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, in particular to a method for collecting microalgae by using protamine flocculation, and the culture solution after flocculation can still be used for cultivating microalgae cells many times, so that the culture water can be circulated to the maximum extent use. Background technique [0002] Microalgae is a kind of single-celled algae, which is widely distributed in land and ocean, has high photosynthetic utilization, short growth cycle, and is an autotrophic plant with strong adaptability to the environment. Microalgae not only has rich nutritional value, but also is a very rich source of high-value chemicals and medicines. Algae cells contain certain components that are difficult to obtain from other organisms, such as unsaturated fatty acids, astaxanthin, β- Carotene and a variety of biologically active substances have therapeutic and medicinal value. At present, polyunsaturated fatty...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 牟海津刘燕妮
Owner OCEAN UNIV OF CHINA