Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for culturing Trichoderma chlamydospore by using Caenorhabditis elegans, and application of chlamydospore

A technology of Caenorhabditis elegans and chlamydospores, applied in the biological field, can solve the problems of less research and application, harsh conditions for artificial fermentation and culture, and low spore production

Inactive Publication Date: 2014-02-05
LINCANG OF YUNNAN TOBACCO +2
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chlamydospores are produced by Trichoderma against adversity conditions. Artificial fermentation culture requires harsh conditions and low spore production, so there are few researches and applications on them at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for culturing Trichoderma chlamydospore by using Caenorhabditis elegans, and application of chlamydospore
  • Method for culturing Trichoderma chlamydospore by using Caenorhabditis elegans, and application of chlamydospore
  • Method for culturing Trichoderma chlamydospore by using Caenorhabditis elegans, and application of chlamydospore

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Utilize Caenorhabditis elegans to cultivate T11-W chlamydospores

[0023] 1 Cultivation of Caenorhabditis elegans

[0024] 1.1 Culture of pregnant worms

[0025] 1.1.1 Culture and collection of E.coli OP50

[0026] 1.1.1.1 Streak inoculation of E.coli OP50 onto LB (1% peptone, 1% NaCl, 0.5% yeast extract, 0.8% agar) medium, culture at 37°C for 16 hours;

[0027] 1.1.1.2 Inoculate a fresh single colony into 200ml liquid LB (1% peptone, 1% NaCl, 0.5% yeast extract) in a 500ml Erlenmeyer flask at 37°C, 200rpm, and incubate for 16h;

[0028] 1.1.1. Centrifuge at 5000rpm for 10min at 34°C, discard the supernatant, concentrate the cells to 25 times the volume, and store at 4°C for later use.

[0029] 1.1.2 Plate culture of Caenorhabditis elegans

[0030] 1.1.2.1 Inoculate 0.5ml of concentrated solution of E.coli OP50 onto the modified NGM plate, shake the plate, disperse the bacterial solution, dry the water on the operating table, and incubate overnight at 37°C...

Embodiment 2T11

[0060] Example 2T11-W Chlamydospore preparation research and application

[0061] 1.1 Preparation of T11-W Chlamydospore Granules

[0062] Using diatomaceous earth as a carrier, together with binder, stabilizer, and pH regulator, according to the optimal ratio, the T11-W chlamydospore granule (2×10 8 cfu / g).

[0063] 1.2 Control of tomato root-knot nematode by this preparation (results are shown in Table 1)

[0064] The nematodes tested were root-knot nematode (Meloidogyne incongnita)

[0065] The test crop is tomato (selected ginkgo strong and abundant)

[0066] The test agents are this preparation and 10% T11-W conidia granules.

[0067] Use soil mixing method. Before the test, soil samples were taken from vegetable sheds in Liying Village, Paddy Town, Shouguang, with nematodes (Meloidogyne incognita was identified as the dominant species). Mix the nematode-carrying soil and this preparation at a weight ratio of 1500:150, 1500:15, 1500:1.5, and pack them in 18×15cm flo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the biotechnical field, and relates to a method for culturing a Trichoderma chlamydospore by using Caenorhabditis elegans, and an application of the chlamydospore. The control efficiency of a preparation (10% T11-W chlamydospore granule) reaches 85.96% when an effective spore amount is 2*10<7>, and is higher than the control efficiency (73.32%) of a 10% T11-W conidiospore granule. The control efficiency of the preparation is 68.96% when an effective spore amount is 2*10<6>, and is obviously higher than the control efficiency (47.15%) of the T11-W conidiospore granule, and a difference between the control efficiencies of the above two granules is large. The T11-W chlamydospore granule has an obviously better control effect on root-knot nematodes than its conidiospore granule.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method and application of cultivating Trichoderma chlamydospores by Caenorhabditis elegans. Background technique [0002] Trichoderma spp is widely present in soil, plant residues, plant roots, plant leaves and seeds, bulb surfaces and animal manure. Such fungi are widely used because they can produce antagonistic substances and parasitize harmful plant pathogenic bacteria. (Yang Chunping, Zhang Jinkang, etc. Development of wettable powder for conidia of Trichoderma viride L24 strain [J]. Journal of Northwest Agricultural Science, 2010,19(9):43-47). Trichoderma inoculants commonly used in production are mostly its conidia preparations, such as ToPshield (Trichoderma harzianum T-22) in the United States, Trichodex (Trichoderma harzianum T39) in Israel and Genyou (Trichoderma harzianum LTR- 2) etc. [0003] Trichoderma T11-W is isolated from eggs of root-knot nematode cucumb...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N3/00A01N63/04A01P5/00C12R1/885
Inventor 王贻莲亚平李纪顺杨合同扈进冬李红梅郭凯陈穗云孙文向许银连尹忠仁
Owner LINCANG OF YUNNAN TOBACCO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com