Algae cell breaking method

A method of crushing and algal cell technology, applied in microorganism-based methods, biochemical equipment and methods, and microbial dissolution, etc., which can solve the problems of local high temperature caused by ultrasonic treatment, reduction of protein activity, and difficulties in industrialization.

Inactive Publication Date: 2014-02-05
HARBIN NORMAL UNIVERSITY
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Problems solved by technology

[0004] (1) Mechanical crushing method: including ultrasonic crushing method and grinding method; the disadvantage of this type of method is that it is suitable for laboratory scale, industrialization is difficult, and ultrasonic treatment will generate local high temperature, and the crushing process needs to be carried out in an ice bath;
[0005] (2) Physical crushing method: including freeze-thaw method and liquid nitrogen grinding method; the disadvantage of this type of method is that it is suitable for laboratory scale, industrialization is difficult, and the crushing time of freeze-thaw method needs to be as long as about 20 hours;
[0006] (3) Chemical osmosis method: including SDS solution extraction method, CaCl 2 Extraction method, cell swelling method, cellulase extraction method; the disadvantage of this type of method is that the crushing time is long, the protein activity is easily reduced, and new substances and chemicals are added to the extract, which increases the difficulty of subsequent purification of phycobiliprotein

Method used

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  • Algae cell breaking method

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Embodiment 1

[0019] Needle-plate corona discharge method: Weigh a certain amount of algae powder and dissolve it in deionized water, place it in the reactor and use the needle-plate corona discharge method for treatment, control the output voltages to be 8, 10, 12kV respectively, and discharge 1, 3, 5, 10min, the frequency is 50Hz;

[0020] Other methods: take the same amount of algae powder separately and use low concentration CaCl 2 Extraction method, SDS extraction method, cell swelling method, cellulase extraction method, repeated freeze-thaw method, liquid nitrogen grinding method, needle plate corona discharge method, ultrasonic crushing method for cell disruption;

[0021] Record and count the results of the early cell crushing treatment by the pin-plate corona discharge method and other methods, and the specific results are shown in Table 1:

[0022] Table 1 is the result of algae cell crushing treatment by different methods

[0023]

[0024] low concentration of CaCl 2 The h...

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Abstract

The invention relates to an algae cell breaking method. The algae cell breaking method comprises the steps of placing algae into a needle-plate corona discharger and then performing high-pressure pulse discharge treatment. The method has the characteristics of high treatment efficiency, no selectivity, no secondary pollution and the like, is strong in pertinence and short in treatment time, can be applied to industrial large-scale production and is environment-friendly and energy-saving.

Description

technical field [0001] The invention relates to the field of phycobiliprotein extraction, and specifically designs a method for crushing algae cells. Background technique [0002] Phycobiliproteins are unique light-harvesting pigment proteins of algae, mainly including phycocyanin, phycoerythrin and allophycocyanin. Algae has strong adaptability, low breeding cost, and is suitable for large-scale production. It is recommended by the Food and Agriculture Organization of the United Nations as the most ideal food for human beings tomorrow. Phycobiliprotein has a wide range of uses. It can be used as a natural pigment in food, cosmetics, dyes and other industries. It can also be made into a fluorescent reagent for clinical medical diagnosis and immunochemistry research fields. Important physiological functions such as body immunity and anti-tumor are important resources for health care products and medicines. [0003] The extraction of phycobiliproteins includes two stages of ...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N1/06C12R1/89
Inventor 赵丽孙文军彭一良
Owner HARBIN NORMAL UNIVERSITY
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