Electrochemical biosensor for detecting penicillin and preparation method and application thereof

A biosensor, electrochemical technology, applied in the field of penicillin detection, can solve the problems of no disclosure, and achieve the effects of small interface resistance, enhanced catalytic efficiency, and large active area

Active Publication Date: 2014-02-05
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there is no disclosure about the use of carbon nanotubes and Fe 3 o 4 /Au nanocomposites u...

Method used

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  • Electrochemical biosensor for detecting penicillin and preparation method and application thereof
  • Electrochemical biosensor for detecting penicillin and preparation method and application thereof
  • Electrochemical biosensor for detecting penicillin and preparation method and application thereof

Examples

Experimental program
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specific Embodiment 1

[0031] An electrochemical biosensor for detecting penicillin, the electrochemical sensor is a three-electrode system, including a counter electrode, a reference electrode and a working electrode, wherein the counter electrode is a platinum wire electrode, and the reference electrode is a saturated calomel electrode. The electrodes are immobilized multi-walled carbon nanotubes (MWCNTs)-Fe 3 o 4 / Glassy carbon electrode of Au magnetic nanoparticles-HRP-penicillin antibody (Pen-Ab).

specific Embodiment 2

[0032] A method for preparing an electrochemical biosensor for detecting penicillin, the principle of the electrochemical sensor detecting penicillin is as follows figure 1 As shown, the specific steps are as follows:

[0033] (1) Carboxylation of carbon nanotubes

[0034] Mix multi-walled carbon nanotubes (MWCNTs) with a mixture of sulfuric acid and nitric acid (3:1, v / v) at a mass-volume ratio of 15 mg: 20 ml, then sonicate for 7-9 hours, and then pass through polyvinylidene fluoride micro Pore ​​filter membrane (pore size: 0.22 μm) vacuum filtration, take the filtrate and wash it with ultrapure water for several times until the filtrate is neutral, that is, the aqueous solution of carboxylated carbon nanotubes is obtained;

[0035] (2) Fe 3 o 4 / Au Composite Nanoparticle Preparation

[0036] Will Fe 3 o 4 Magnetic nanoparticles (can be prepared by co-precipitation method and thermal decomposition method or directly purchased at Xi'an Gold Magnetic Nano Biotechnology C...

specific Embodiment 3

[0042] A based on multi-walled carbon nanotubes (MWCNTs)-Fe 3 o 4 / Au Magnetic Nanoparticles-HRP-Penicillin Antibody (Pen-Ab) Electrochemical Biosensor with Glassy Carbon Electrode to Detect Penicillin Concentration

[0043] The carboxylated carbon nanotubes prepared in the above-mentioned specific example two, Fe 3 o 4 / Au-HRP-Ab was sequentially modified onto the surface of the bare glassy carbon electrode as the working electrode, the platinum electrode was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode to form an electrochemical biosensor with a three-electrode system. When the target analyte penicillin exists in the detection system, the target will react specifically with the antibody immobilized on the surface of the electrode. As the concentration of the target increases, more immune complexes will hinder the oxidation of hydroquinone. Hinder the transfer effect of electrons on the surface of the electrode and ...

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Abstract

The invention discloses an electrochemical biosensor for detecting penicillin and a preparation method and application thereof. The electrochemical biosensor is characterized in that a working electrode is a magnetic glassy carbon electrode immobilized with MWCNTs-Fe3O4/Au-HRP-Ab. The preparation method comprises the following steps: carrying out carboxylation on a multi-walled carbon nanotube, preparing Fe3O4/Au composite nano particles, preparing a Fe3O4/Au magnetic nano particle-HRP-penicillin antibody, sequentially modifying the carbon nanotube subjected to carboxylation and Fe3O4/Au-HRP-Ab to the surface of the glassy carbon electrode of the carbon nanotube, forming the electrochemical biosensor by taking a platinum electrode as a counter electrode and taking a saturated calomel electrode as a reference electrode, immersing the electrochemical biosensor into a sample to be detected, and determining the concentration of the penicillin in the sample to be detected according to a quantitative relation between a corresponding current value and the concentration of the penicillin. The electrochemical biosensor has the advantages of high sensitivity, high selectivity, high accuracy and high detection speed.

Description

technical field [0001] The invention relates to penicillin detection technology, in particular to a method based on MWCNTs-Fe 3 o 4 Au / Au electrochemical biosensor for detecting penicillin and its preparation method and application. Background technique [0002] Penicillin is a β-lactam antibiotic widely used in the treatment of bovine mastitis and the prevention of bacterial infection. However, due to the unreasonable use of human beings, it often leads to a large amount of penicillin residues in biological tissues, especially in food. cause potential harm. First of all, excessive penicillin content will produce resistance to the microorganisms in the intestinal tract of humans and animals, causing flora imbalance, which will lead to a series of diseases such as allergic reactions. Second, after the use of penicillin to prevent and treat disease in dairy cows, the antibiotic will accumulate in the body. Long-term consumption of milk containing excessive penicillin will ...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 潘道东李建龙孙杨赢曾小群曹锦轩陈伟李桦
Owner NINGBO UNIV
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