Electrochemical biosensor for detecting penicillin and preparation method and application thereof

Abstract

The invention discloses an electrochemical biosensor for detecting penicillin and a preparation method and application thereof. The electrochemical biosensor is characterized in that a working electrode is a magnetic glassy carbon electrode immobilized with MWCNTs-Fe3O4 / Au-HRP-Ab. The preparation method comprises the following steps: carrying out carboxylation on a multi-walled carbon nanotube, preparing Fe3O4 / Au composite nano particles, preparing a Fe3O4 / Au magnetic nano particle-HRP-penicillin antibody, sequentially modifying the carbon nanotube subjected to carboxylation and Fe3O4 / Au-HRP-Ab to the surface of the glassy carbon electrode of the carbon nanotube, forming the electrochemical biosensor by taking a platinum electrode as a counter electrode and taking a saturated calomel electrode as a reference electrode, immersing the electrochemical biosensor into a sample to be detected, and determining the concentration of the penicillin in the sample to be detected according to a quantitative relation between a corresponding current value and the concentration of the penicillin. The electrochemical biosensor has the advantages of high sensitivity, high selectivity, high accuracy and high detection speed.

Description

technical field

[0001] The invention relates to penicillin detection technology, in particular to a method based on MWCNTs-Fe 3 o 4 Au / Au electrochemical biosensor for detecting penicillin and its preparation method and application. Background technique

[0002] Penicillin is a β-lactam antibiotic widely used in the treatment of bovine mastitis and the prevention of bacterial infection. However, due to the unreasonable use of human beings, it often leads to a large amount of penicillin residues in biological tissues, especially in food. cause potential harm. First of all, excessive penicillin content will produce resistance to the microorganisms in the intestinal tract of humans and animals, causing flora imbalance, which will lead to a series of diseases such as allergic reactions. Second, after the use of penicillin to prevent and treat disease in dairy cows, the antibiotic will accumulate in the body. Long-term consumption of milk containing excessive penicillin will ...

Images