Quick hormone-free cultivation method of dendrobium huoshanense seedlings

A technology of Dendrobium huoshanensis and a cultivation method, applied in the field of plant biology, can solve the problems of high cost of seedlings, long reproduction cycle, influence on the quality of Dendrobium huoshan, and achieve the effects of maintaining quality, low cost of culture medium, and shortening breeding cycle of seedlings

Inactive Publication Date: 2014-02-19
HEFEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Exogenous hormones affect the quality of Dendrobium, the seedling reproduction cycle is long (about 8 months), and the cost of seedlings is high, each priced at 1 yuan

Method used

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  • Quick hormone-free cultivation method of dendrobium huoshanense seedlings
  • Quick hormone-free cultivation method of dendrobium huoshanense seedlings
  • Quick hormone-free cultivation method of dendrobium huoshanense seedlings

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Embodiment Construction

[0044] A hormone-free rapid culture method for Dendrobium huoshanense seedlings, comprising the following steps:

[0045] (1) Protocorm culture

[0046] ① Selection of explants

[0047] Huoshan Dendrobium fruit pods that have grown for about 100 days and have not yet fully matured are picked from Huoshan, soaked in 1% detergent for 10 minutes, and then scrubbed with running water;

[0048] ②Sterilization

[0049] Put the washed fruit pods into a sterile beaker on an ultra-clean workbench, soak them in 75% alcohol for 30 seconds, rinse them once with sterile water, then soak them with 5% sodium hypochlorite solution for 20 minutes, and rinse them twice with sterile water.

[0050] ③ Inoculation and culture

[0051] Strictly aseptic operation, the scalpel is cut longitudinally along the fruit pod, and the milky white powdery immature embryos are directly inoculated on No. 1 medium: nutrient element mother solution 100ml / L + potato puree 100g...

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Abstract

The invention discloses a quick hormone-free cultivation method of dendrobium huoshanense seedlings. The method comprises the steps as follows: dendrobium huoshanense capsules which grow about 100 days and are not fully matured are picked in Huoshan, and ivory powdered young embryos in the capsules are taken as explants; a novel optimized culture medium is adopted, and compared with a conventional MS culture medium, dosage of chemicals of inorganic salt and the like as well as sugar is smaller, edible substances or waste materials such as mashed yam, bran paste, banana skin homogenate and the like are added in the culture medium respectively at different cultivation stages, a carbon source adopts commercially available edible sugar, and hormone is not added; seedlings are healthy and strong after cultivation for six months, each seedling is 3-4 cm high and has 3-4 sections, 6-8 leaves and 4-5 roots, the root length is 2-4 cm, and the transplant survival rate is higher than 95%; and later, the seedlings are transferred to a natural environment of an original area for cultivation, so that wild dendrobium nobile quality can be maintained. With the adoption of the quick hormone-free cultivation method of the dendrobium huoshanense seedlings, the young embryo breeding rate is high, the seedling breeding period is shortened, the culture medium cost is low, the utilization values of yams, brans, banana skin (waste materials) are improved, and the optimized culture medium is suitable for large-scale industrial production.

Description

Technical field [0001] The present invention involves a method of rapid cultivation of Dendrobium seedlings from Huoshan, which belongs to the field of plant biotechnology. Background technique [0002] Dendrobium Huo Shan belongs to the national first -level protection of rare Chinese medicinal materials."Compendium of Materia Medica" records: Dendrobium Huo Shan has strong yin and essence, thick gastrointestinal, and inadequate inside, flat stomach qi, long muscles, puzzle removal, and extending the effect of being prolonged.Dendrobium Huo Shan is rich in polysaccharides, alkaloids, amino acids and other substances.Modern pharmacological studies have shown that Dendrobium Huo Shan has anti -tumor, lowering blood sugar, can improve the body's immunity, both medicinal and nutritious and health care. [0003] The growth environment of Dendrobium Huo Shan is demanding, naturally breeding slowly, the seeds are very small, no embryo, and it is difficult to germinate in the natural st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 樊家荣
Owner HEFEI UNIV
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