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Evening primrose oil extraction method

An extraction method and grass oil technology, which are applied in the production of fat/fat and fat production, can solve the problems of difficult research, low oil yield, and difficult demulsification and separation of emulsions.

Inactive Publication Date: 2014-02-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it is also a difficult process to study, especially for oils with low oil content, there are technical problems such as low oil yield, and the emulsion formed by protein and oil after enzymatic hydrolysis is difficult to break and separate.

Method used

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  • Evening primrose oil extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Screening test for optimal parameters of evening primrose seed ultrasonic wall-breaking pretreatment

[0025] 1 Materials and methods

[0026] 1.1 Materials and reagents

[0027] Evening Primrose Seeds Jilin Province Wanshou Pine Nuts Food Company

[0028] Alcalase 2.4L Novozymes

[0029] 1.2 Main instruments and equipment

[0030] pHS-25 acidity meter Shanghai Weiye Instrument Factory

[0031] Electronic Analytical Balance Merlot-Toledo Instruments (Shanghai) Co., Ltd.

[0032] Centrifuge Beijing Medical Centrifuge Factory

[0033] Precision Electric Mixer Jiangsu Jintan City Ronghua Instrument Manufacturing Co., Ltd.

[0034] Electric constant temperature water bath Yuyao Dongfang Electrical Instrument Factory

[0035] Semi-automatic azotometer Shanghai Xinjia Electronics Co., Ltd.

[0036] Digester Shanghai Fiber Inspection Instrument Co., Ltd.

[0037] Hammer Mill China Tianjin Test Instrument Co., Ltd.

[0038] Soxhlet Extractor Tianjin Glass I...

Embodiment 2

[0073] Example 2: Screening test for optimal parameters of enzymatic hydrolysis of evening primrose oil extracted by aqueous enzymatic method

[0074] Based on the best ultrasonic wall-breaking pretreatment determined in Example 1 to extract the technology of evening primrose oil, a single factor enzymolysis test is carried out to determine the enzymolysis process parameters (material-liquid ratio, enzyme addition, enzymolysis temperature, enzymolysis time) ) range. Taking the total oil extraction rate as the investigation index, a response surface design experiment with 4 factors and 3 levels was carried out.

[0075] 1 Materials and methods

[0076] 1.1 Materials and reagents

[0077] Evening Primrose Seeds Jilin Province Wanshou Pine Nuts Food Company

[0078] protex-6L alkaline endoprotease Danish novo company

[0079] 1.2 Main instruments and equipment

[0080] pHS-25 acidity meter Shanghai Weiye Instrument Factory

[0081] Electronic Analytical Balance Merlot-Toled...

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Abstract

The invention relates to an evening primrose oil extraction method, and belongs to the plant oil extraction processing technology. The method comprises: (1) carrying out cleaning and mechanical crushing on evening primrose seeds, adding water to mix to obtain a mixing solution, and carrying out an ultrasonic wall breaking pre-treatment on the mixing solution by using an ultrasonic cell disrupter; (2) adding protease to the ultrasonic treated mixing solution to carry out enzyme digestion to obtain an enzyme digestion solution; and (3) carrying out centrifugal separation on the enzyme digestion solution to obtain free oil, an emulsion, a hydrolysis solution and residue, carrying out repeated water washing and centrifugal separation on the residue to obtain the free oil and the emulsion, carrying out emulsion breaking on the emulsion to obtain the free oil, and mixing all the obtained free oils to obtain the evening primrose oil. According to the present invention, the method has characteristics of simple equipment, safe operation, low enzyme consumption, short enzyme digestion time and high oil extraction efficiency, the obtained evening primrose oil has characteristics of no solvent residue and high nutritional value, and the total oil extraction rate can achieve about 84.32%.

Description

technical field [0001] The invention belongs to the technical field of vegetable oil extraction and processing, and mainly relates to a method for extracting evening primrose oil. Background technique [0002] Evening primrose oil can treat a variety of diseases, regulate blood lipids, and have significant curative effects on coronary artery infarction, atherosclerosis, and cerebral thrombosis caused by high cholesterol and high blood lipids. It can also treat multiple sclerosis, diabetes , obesity, rheumatoid arthritis and schizophrenia. Primrose oil can be used as a high-quality product of nutritional health oil. [0003] At present, the common preparation methods of evening primrose oil include solvent extraction, pressing, supercritical CO 2 extraction method. Supercritical CO 2 The extraction method can effectively overcome the solvent residue of the solvent extraction method and the low yield of the pressing method, but this method has high requirements on equipme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/04C11B1/00
Inventor 江连洲李杨马文君齐宝坤王中江隋晓楠冯红霞曹亮
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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