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Three-step enzymatic hydrolysis method used for producing forage saccharomyces cerevisiae cells with broken-down walls

A technology of Saccharomyces cerevisiae and Saccharomyces cerevisiae is applied in the field of preparation of wall-breaking yeast cells for feed, and can solve the problems of chemical residues, high noise of mechanical methods, and few applications of enzymatic methods.

Inactive Publication Date: 2014-02-19
GUANGZHOU NIUYILE TRADING
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breakage of yeast cell wall is mainly through mechanical method, chemical method and enzymatic method, but enzymatic method is rarely used, because the cracking effect of single enzymatic method is poor
The mechanical method is noisy, and the chemical method produces a large amount of sewage, which easily leads to the residue of chemicals in the feed.

Method used

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  • Three-step enzymatic hydrolysis method used for producing forage saccharomyces cerevisiae cells with broken-down walls
  • Three-step enzymatic hydrolysis method used for producing forage saccharomyces cerevisiae cells with broken-down walls
  • Three-step enzymatic hydrolysis method used for producing forage saccharomyces cerevisiae cells with broken-down walls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Application of Example 1 Feeding Broken Yeast Cells

[0027] 1 Materials and methods

[0028] 1.1 Experimental animals and grouping

[0029] Three-way hybrid pigs (male parent: Duroc, female parent: grown-up), 24 1-day-old newborn piglets were randomly divided into 3 groups, 8 pigs in each group (the data were deleted after death in the middle). Blank control group, test group 1 (administered synbiotics 1g / day), group 2 (10g / day; yes. The feed is milk replacer powder.

[0030] 1.2 Broken yeast cells for feed in the test product (provided by Beijing Yangming Yuanjing Biotechnology Co., Ltd.)

[0031] 1.3 Test time and place

[0032] June 9, 2009 - July 14, 2009

[0033] Beijing Resources Asia-Pacific Breeding Pig Co., Ltd.

[0034] 1.4 Test method

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PUM

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Abstract

The invention provides a forage symbiotics mild production method used for producing saccharomyces cerevisiae cells with broken-down walls, wherein a product of the forage symbiotics mild production method comprises yeast metabolites such as yeast protein, yeast steroid, yeast polysaccharide, and yeast nucleic acid. The saccharomyces cerevisiae cells with broken-down walls can be used a novel forage additive so as to solve problems of low protein utilization ratio, high diarrhea ratio, and high morbidity in the field of animal husbandry. According to the forage symbiotics mild production method, yeast bacteria are obtained via submerged liquid state fermentation, and then the saccharomyces cerevisiae cells with broken-down walls are obtained via three-step enzymatic hydrolysis method. Production cost is low, viable count is high, and the saccharomyces cerevisiae cells with broken-down walls is a green forage additive with stability and high efficiency. Process route is disclosed in a attached figure.

Description

technical field [0001] The invention relates to a preparation method of broken yeast cells for feeding. technical background [0002] Yeast can convert low-quality protein into high-quality protein and provide high-quality protein feed for animals. At the same time, yeast cell wall cells contain a large amount of yeast protein, yeast nucleic acid, yeast steroids and other nutrients, which can regulate immunity, improve animal disease resistance, reduce The use of antibiotics is an excellent green feed additive. The breakage of yeast cell wall is mainly through mechanical method, chemical method and enzymatic method, but the application of enzymatic method is seldom, because the cracking effect of single enzymatic method is poor. The mechanical method is noisy, and the chemical method produces a large amount of sewage, which easily leads to the residue of chemicals in the feed. [0003] The method is to use Saccharomyces cerevisiae approved by the Ministry of Agriculture to...

Claims

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Application Information

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IPC IPC(8): C12N1/06A23K1/16A23K1/18C12R1/865
Inventor 张江胡祥
Owner GUANGZHOU NIUYILE TRADING
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