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Chicken infectious bronchitis live vaccine, heat-resisting protective agent and preparation method of the vaccine

A technology of heat-resistant protective agent and bronchitis, which is applied in antiviral agents, pharmaceutical formulas, medical preparations of non-active ingredients, etc., can solve the problems of epidemic epidemics, lagging development, long-term storage of vaccines and long-distance transportation restrictions, etc., to achieve Easy to store and reduce the effect of great loss

Inactive Publication Date: 2014-02-26
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development of domestic traditional animal vaccine protective agents is relatively lagging behind, which greatly restricts the long-term storage and long-distance transportation of vaccines. In addition, the grassroots epidemic prevention departments lack necessary freezing and cold storage facilities, which may easily cause epidemics due to immunization failure.
The heat-resistant protection technology of developed countries in the world has been widely used. Most of the freeze-dried vaccines can be stored at 2-8°C, which greatly reduces the storage conditions of the vaccines and improves the stability and use effect of the vaccines. Detailed report of the recipe

Method used

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  • Chicken infectious bronchitis live vaccine, heat-resisting protective agent and preparation method of the vaccine
  • Chicken infectious bronchitis live vaccine, heat-resisting protective agent and preparation method of the vaccine
  • Chicken infectious bronchitis live vaccine, heat-resisting protective agent and preparation method of the vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Screening of Chicken Infectious Bronchitis Kidney Type K136 Strain

[0027] From 2003 to 2005, our company collected suspected chicken infectious bronchitis virus isolates from different chicken farms in my country. The sick chickens mainly manifested as lack of energy, coughing, and often heard grunting. Pathological autopsy found that there were obvious lesions in the kidneys in addition to small bleeding spots in the tracheal ring. After preliminary identification, 4 strains of renal-type infectious bronchitis virus were obtained. They were named CK / IBV / QD03 strain, CK / IBV / ZC03 strain, CK / IBV / YT04 strain and CK / IBV / SD05 strain respectively. Use 10-day-old SPF chicken embryos to carry out continuous subculture to attenuate the four strains. In each generation, 4 chicken embryos were inoculated through the allantoic cavity, incubated at 37°C, and the chicken embryos were frozen to death 36 hours after inoculation, and the allantoic fluid was harvested for th...

Embodiment 2

[0036] Embodiment 2: K136 strain safety detection

[0037] 1. Determination of toxicity of K136 strains of different generations on chicken embryos

[0038] Take the E2, E4, E7, E10, E15 generation virus seeds of the K136 strain, make appropriate multiple dilutions, inoculate 10-day-old SPF chicken embryos through the allantoic cavity, 0.1 mL per embryo, and set a blank control group at the same time, The cavity was inoculated with 0.1 mL of physiological saline, and incubated at 37°C. The chicken embryos that died within 24 hours were discarded, and after 144 hours of incubation, the embryos were taken out to observe the development of the chicken embryos. Calculation of EID according to the Reed-Muench method 50 , the poisonous price of E2, E4, E7, E10, E15 generation poison species is respectively: 10 6.7 、10 6.8 、10 6.5 、10 6.6 、10 6.8 EID 50 / 0.1mL. The test results showed that the K136 strain proliferated stably on chicken embryos, and there was no reversion of ...

Embodiment 3

[0044] Example 3: Evaluation of immune efficacy of different generations of K136 strain

[0045] Use the E2, E4, E7, E10, E15 generation K136 strains to breed chicken embryo allantoic fluid in 5×10 2.0 EID 50 Dosage, intranasal drops for 1-day-old SPF chickens, 14 days after immunization, together with control chickens, each chicken used 10 3.68 EID 50 / 0.1mL of the isolated virulent CK / IBV / SD05 strain 0.05mL for eye drops and nasal drops respectively. On the 5th day after the challenge, the tracheal swabs of each chicken were collected and inoculated into the 10-day-old SPF chicken embryo 5 through the allantoic cavity. Embryo, 0.1mL / embryo, incubated for 144 hours. The results showed that the toxicity rate of the immune group was between 0 / 10-1 / 10, and the protection rate was between 9 / 10-10 / 10; the toxicity rate of the control group was 10 / 10. It shows that the immunogenicity of IBV-K136 strain virus seeds is basically the same within 15 generations, and they all have g...

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Abstract

The invention discloses a chicken infectious bronchitis live vaccine, a heat-resisting protective agent and a preparation method of the vaccine. The live vaccine comprises an antigen and the heat-resisting protective agent. The antigen is a chicken kidney-type infectious bronchitis virus K136 strain having an accession number of CCTCC-V201320. The preparation method includes performing amplification culture to the infectious bronchitis virus K136 strain by using chicken embryos and preparing the heat-resisting protective agent; mixing the amplification culture fluid with the heat-resisting protective agent; and subjecting the mixed solution obtained to freeze-drying to obtain the vaccine. The vaccine solves a predicament that nearly no vaccine is available for vaccine control of the chicken kidney-type infectious bronchitis epidemic in our country at present, and fills the gap of preparation of heat-resisting live vaccines for the chicken kidney-type infectious bronchitis in our country. Compared with live vaccines at present, the vaccine provided by the invention is stored at 2-8 DEG C in the period of validity, and can be stored, transported and used conveniently.

Description

technical field [0001] The invention belongs to the technical field of new biomedicine for livestock and poultry, and in particular relates to a live chicken infectious bronchitis vaccine, a heat-resistant protective agent and a preparation method thereof Background technique [0002] Avian Infectious Bronchitis (IB) is an acute and highly contagious infectious disease of chickens, which mainly affects the respiratory system, genitourinary system and digestive system. The disease was first reported by Schalk and Hawn in the U.S. Clinically, the respiratory symptoms of coughing, sneezing and tracheal rales in chickens were the main ones, and then it occurred in various countries in the world. In 1936, Beach and Schalm determined that the pathogen was a virus (respiratory IBV). Named as chicken infectious bronchitis virus (Infectious Bronchitis Virus, IBV), classified into Coronaviridae, is a representative strain of coronavirus. [0003] Renal infectious bronchitis is a path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61K47/36A61P31/14A61K47/32A61K47/18A61K47/22
Inventor 孙美玉何召庆吴全忠杨保收陈冰邢婧珉李云霞梁武李守军
Owner TIANJIN RINGPU BIO TECH