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Bacillus amyloliquefaciens Y10 and application thereof

A technology of amylolytic spores and bacillus, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve problems such as no related reports, achieve broad social benefits, improve degradation efficiency, and be practical and economical Effect

Active Publication Date: 2014-02-26
河北绿茵生化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Bacillus amyloliquefaciens has been studied in recent years, and its metabolites are rich, mainly used in the inhibition of fungal and bacterial activities, such as patent CN201110389068. The application of Bacillus in the prevention and treatment of peanut fruit rot is rare, but there is no relevant report on the anaerobic fermentation of organic waste such as kitchen waste

Method used

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  • Bacillus amyloliquefaciens Y10 and application thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and preservation of Bacillus amyloliquefaciens Y10

[0021] Bacillus amyloliquefaciens Y10 was isolated from the soil around the canteen of Hebei Academy of Sciences by gradient dilution method.

[0022] The specific method is: Weigh 10 g of soil from the place polluted by leftovers and leftovers around the cafeteria of the Hebei Academy of Sciences, add it to a triangular flask filled with 90 mL of sterile water and glass beads, shake it on a shaker for 30 min (150 r / min), serially diluted to make 10 -5 、10 -6 Dilute the suspension, draw 100 μL each, add it to the primary screening medium plate, spread it evenly with a coating bar, and repeat each treatment 3 times. The above-mentioned petri dishes were placed in a constant temperature incubator at 25 °C for 2-3 days. Select a single colony with a large transparent circle and grow quickly, transfer it to the slant of the LB test tube, and store it at 4 °C. The isolated strains were cultured in...

Embodiment 2

[0036] Example 2 Determination of Bacillus amyloliquefaciens Y10 enzyme activity

[0037] The isolated strains were placed in LB liquid medium and cultured in an incubator at 25 °C for 72 h. The fermentation broth was centrifuged at 4 °C and 8000 r / min for 10 min, and the supernatant was taken to determine amylase, protease and fat Enzyme activity.

[0038] The measurement methods are respectively used:

[0039] Determination method of amylase: national standard GB / T 24401-2009.

[0040]Definition of enzyme activity: 1 g of solid enzyme powder (or 1 mL of liquid enzyme), at 60 °C and pH 6.0, liquefies 1 g of soluble starch for 1 hour, which is 1 enzyme activity unit, expressed in u / g (u / mL) said.

[0041] Protease determination method: national standard GB / T 23527-2009 (Forint method).

[0042] Definition of enzyme activity: 1 g of solid enzyme powder (or 1 mL of liquid enzyme), under certain temperature and pH conditions (this patent uses 40°C, pH 7.0), hydrolyzes casein...

Embodiment 3-7

[0046] Example 3-7 Application of Bacillus amyloliquefaciens Y10 in anaerobic fermentation of kitchen waste

[0047] A 5 L fully mixed anaerobic fermentation device was used, with kitchen waste as raw material, the inoculum was from a normal fermentation biogas digester, the inoculum amount was 20-30% (volume ratio), and the final anaerobic fermentation liquid TS was 6-10% ( TS, also known as dry matter concentration, refers to placing a certain amount of fermentation feed liquid in an oven at 105 °C and drying it to a constant weight (the percentage of dry matter in the total weight), according to 5-20% of the total amount of anaerobic fermentation liquid Insert Bacillus amyloliquefaciens Y10 bacterial liquid, the total number of bacteria contained in the bacterial liquid is ≥10 9 individual / mL. Fermentation at room temperature (25±1 ℃) for 50 days increased biogas production by 20-38%. Fermentation at medium temperature (35±1 ℃) for 30 days increased biogas production by ...

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Abstract

The invention relates to a Bacillus amyloliquefaciens Y10 and application thereof. The Bacillus amyloliquefaciens Y10 is collected by China General Microbiological Culture Collection Center in China Committee for Culture Collection of Microorganisms on October 18th, 2013, and the collection number is CGMCC No.8362. The Bacillus amyloliquefaciens Y10 provided by the invention is separated from soil; the optimal growth temperature is 25-35 DEG C, and the pH value is 6.8-7.5; and the Bacillus amyloliquefaciens Y10 has the capacity for producing amylases, proteases and lipases. When being used for anaerobic fermentation of kitchen waste, the Bacillus amyloliquefaciens Y10 enhances the marsh gas production rate by 15-40%. The Bacillus amyloliquefaciens Y10 can effectively enhance the anaerobic fermentation hydrolysis and marsh gas production efficiency of waste or wastewater containing proteins, starch, fat and the like, and has important meanings for promoting conversion of organic waste into harmless substances, resources and energy sources.

Description

technical field [0001] The present invention relates to a kind of Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) Y10 and its application belong to the technical field of microbial fermentation. Background technique [0002] With the development of the economy and the improvement of people's living standards, the output of kitchen waste in my country has grown rapidly. According to statistics, Chinese cities produce no less than 60 million tons of food waste every year, and the harmless treatment rate is less than 5%. Some food waste is processed into "gutter oil" without treatment and enters the market or underground farms as feed. , seriously threatening the lives and health of the people. Since 2012, the government has attached great importance to the industry, and relevant policies have been promulgated intensively. [0003] At present, food waste mainly adopts several treatment methods such as incineration, composting, landfill, and making feed. Among them,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/00C12P5/02C02F3/34C12R1/07
CPCY02E50/30
Inventor 程辉彩崔冠慧张丽萍习彦花张根伟尹淑丽董玉兰张飞燕段普凡李书生
Owner 河北绿茵生化科技有限公司
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