Method for extracting fish oil from fish heads and fish skin
A fish skin and fish head technology is applied in the production of fat oil/fat, fat production, etc., which can solve the problems of unstable water content of fish meal, environmental pollution, and poor utilization of waste by-products, etc., to improve the purity of fish oil and the extraction effect. Good results
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Embodiment 1
[0012] A method for extracting fish oil from fish heads and fish skins, comprising the following steps:
[0013] (1) Raw materials are crushed. Freeze the cleaned fish head and fish skin at -25°C for 16 hours, add 1 / 20 of the weight of the raw materials in water, grind them, and then put them into a homogenizer to homogenize to obtain a homogenate;
[0014] (2) Enzymatic hydrolysis, put the homogenate into a constant temperature pot, add water 8 times the weight of the homogenate, control the temperature at 45°C, add neutral protein, react for 12 hours and concentrate;
[0015] (3) Organic solvent extraction, the enzymatic hydrolysis product was dried, added cyclohexane 10 times its weight, and centrifuged, extracted 3 times, and the cyclohexane layer was collected and combined;
[0016] (4) Concentration and purification, concentrating and recovering the organic solvent to obtain crude fish oil, washing the crude fish oil with 10% sodium chlorate solution for 1-2 times to obt...
Embodiment 2
[0018] A method for extracting fish oil from fish heads and fish skins, comprising the following steps:
[0019] (1) Raw materials are crushed. Freeze the cleaned fish head and fish skin at -20°C for 20 hours, add 1 / 30 of the weight of the raw materials in water, grind them, and then put them into a homogenizer to obtain a homogenate;
[0020] (2) For enzymatic hydrolysis, put the homogenate into a constant temperature pot, add water 5 times the weight of the homogenate, control the temperature at 50°C, add neutral protein, and react for 10 hours;
[0021] (3) Organic solvent extraction, the enzymatic hydrolysis product was dried, added cyclohexane 5 times its weight, centrifuged, extracted 3 times, and the cyclohexane layer was collected and combined;
[0022] (4) Concentration and purification, concentrating and recovering the organic solvent to obtain crude fish oil, washing the crude fish oil with 10% sodium chlorate solution for 1-2 times to obtain fish oil.
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