Microfluidic devices and their uses

A microfluidic device and microfluidic channel technology, applied in enzymology/microbiology devices, fluid controllers, biochemical cleaning devices, etc., can solve problems such as the need for improvement of microfluidic chips

Active Publication Date: 2016-08-17
BGI SHENZHEN CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, current microfluidic chips still need to be improved

Method used

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  • Microfluidic devices and their uses
  • Microfluidic devices and their uses
  • Microfluidic devices and their uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1 Genetic identification of fetal nucleated erythrocytes

[0062] 1.15 mL of peripheral blood from the cubital vein of pregnant women at 8-14 weeks of pregnancy, anticoagulated with EDTA-K2, stored with shaking at 4°C, and tested within 24 hours.

[0063] 1.2 Separation of fetal nucleated erythrocytes: Two steps are used for separation. Firstly, separate the red and white blood cells according to the size difference, such as the chip structure in 2.1, remove most red blood cells; pass the recovered cells into the magnetic separation chip, and separate the red and white blood cells by using the magnetic difference of the red and white blood cells.

[0064] The structure of the first chip: the spacing between microcolumns is 15 μm, and the relative offset of each row of microcolumns is 6.75 μm. Using deep reactive ion etching technology to build microstructures on silicon wafers, the depth of the channels is 150 μm, and sealing them with glass wafers.

[0065] T...

Embodiment 2

[0071] Example 2: Identification of chromosomal aneuploidy in fetal nucleated erythrocytes

[0072] 2.1 Sampling: Same as 1.1

[0073] 2.2 Separation of fetal nucleated red blood cells:

[0074] The structure of the first chip used: as Figure 5 The Nautilus chip in has one sample inlet and 6 sample outlets. The width of the inlet channel is 0.22mm, and the curvature of the channel is gradually enlarged according to a certain ratio. The channel width at the outlet is 3.88mm. The chip channel structure su-8 negative photoresist is used to make a mask by photolithography, and then the PDMS prepolymer is mixed and cast on the mold, and it is formed by turning over the mold. Use glass slides for sealing.

[0075] The structure of the second chip used: as Figure 6 . There are two entrances and two exits. The channel width is 0.5mm and the length is 50mm. The chip channel structure su-8 negative photoresist is used to make a mask by photolithography, and then the PDMS prepol...

Embodiment 3

[0082] Example 3: Screening of circulating tumor cells (CTCs) in cancer patients

[0083] 3.1 Sampling: Take 10 mL of peripheral blood from the cubital vein of tumor patients and anticoagulate with EDTA-K2. Blood samples were stored at 4°C and tested within 24 hours.

[0084] 3.2 Separation of CTCs: Two-step chip was used for separation. The first step is to separate cells according to their size, such as figure 1 In the chip structure, most of the red blood cells and some extracellular cells were removed; in the second step, EpCAM antibody was used to capture CTCs cells. specifically,

[0085] The structure of the first chip: the spacing between the microcolumns is 20 μm, and the relative offset of each row of microcolumns is 6 μm. Using deep reactive ion etching technology to construct microstructures on silicon wafers, the channel depth is 150mm, and sealing with glass wafers.

[0086] Configuration of the second chip: CTCs were captured using Miltenyi LS / MS Column (M...

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Abstract

Microfluidic devices and uses thereof are provided, in particular, microfluidic devices, methods for determining whether a biological sample has genetic variation, and systems for determining whether a biological sample has genetic variation are provided. Wherein, the microfluidic device has a microfluidic channel suitable for separating single cells.

Description

technical field [0001] The present invention relates to a microfluidic device and its use, in particular, to a microfluidic device, a method for determining whether a biological sample has genetic variation, and a system for determining whether a biological sample has genetic variation. Background technique [0002] Microfluidic chip technology originated in the field of analytical chemistry. It integrates the basic operating units of sample preparation, reaction, separation, and detection involved in the fields of biology, medicine, and chemistry into chips of several square centimeters through microfabrication technology. A network of microchannels is used to run through the entire system with controllable fluids, which is used to replace various functions of conventional analytical laboratories. Microfluidic technology has shown great advantages in cell separation, nucleic acid extraction, purification, PCR amplification, etc. [0003] However, current microfluidic chips...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12Q1/02C12Q1/68
CPCC12M47/04B01L3/502746B01L3/502753B01L2200/0652B01L2300/0861B01L2300/0883B01L2400/086
Inventor 王琳琳刘笔锋吴逵侯勇陈璞董迎松宋卢挺徐迅
Owner BGI SHENZHEN CO LTD
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