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Method for immobilizing carbonic anhydrase by use of modified bagasse

A technology of carbonic anhydrase and carbonic anhydrase meter is applied in directions such as being fixed on/in an organic carrier, which can solve the problems of insufficient stability and loss of activity, and achieve the advantages of reducing usage, simple process, and saving resources and energy. Effect

Active Publication Date: 2014-03-12
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But enzymes also have some disadvantages, especially when used as industrial catalysts
Because the main component of the enzyme is protein, when it is in high temperature, strong acid-base and organic solvent conditions, its properties are not stable enough, and it is easy to lose activity in the reaction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Soak 100g of bagasse (the variety of sugarcane is Taisugar 22) in 1% NaOH for 25 hours, then use 1% HCl to neutralize the pH value of the solution to 7.0, filter, and bake at 55°C dry, that is, the activation of bagasse is completed; the activated bagasse is put into a solution of 2,4,6-trichloro-1,3,5-triazine with a mass concentration of 1.5%, stirred for 2 hours, filtered, and the filter residue is collected Finally, wash with water of 8 times the volume of the filter residue in order to remove excess 2,4,6-trichloro-1,3,5-triazine.

[0018] Add 4 mg of carbonic anhydrase per gram of modified bagasse, and add carbonic anhydrase (model: 9001-03-0) from Shanghai Yiyi Biological Co., Ltd. to the modified bagasse; at a temperature of 45°C After oscillating for 6 hours under the same conditions, put it in a freezer at 4±1°C for 18 hours, filter, and wash the filter residue with 0.2mol / L phosphate buffer solution and distilled water repeatedly until the enzyme-free protein...

Embodiment 2

[0021] Soak 100g of bagasse (the variety of sugarcane is Guitang 22) in NaOH with a mass concentration of 0.5% for 20 hours, then use 0.5% HCl to neutralize the pH value of the solution to 6.9, filter, and store at 30°C Drying, that is, the activation of bagasse is completed; the activated bagasse is put into a solution of 2,4,6-trichloro-1,3,5-triazine with a mass concentration of 0.5%, stirred for 1 hour, filtered, and collected After filtering the residue, wash with water 5 times the volume of the filter residue, in order to remove excess 2,4,6-trichloro-1,3,5-triazine.

[0022] Add 3 mg of carbonic anhydrase per gram of modified bagasse, add carbonic anhydrase (model: 9001-03-0) from Shanghai Yiyi Biological Co., Ltd. in the modified bagasse; Shake at 30°C for 1 hour, put it in a freezer at 4±1°C for 12 hours, filter, and wash the filter residue repeatedly with 0.2mol / L phosphate buffer solution and distilled water until the enzyme-free protein is washed out, and the modif...

Embodiment 3

[0025] Soak 100g of bagasse (the variety of sugarcane is Guitang 20) in NaOH with a mass concentration of 2% for 30 hours, then neutralize the pH value of the solution to 7.1 with HCl with a mass concentration of 2%, filter, and store at 80°C After drying, the activation of bagasse is completed; the activated bagasse is put into a solution of 2,4,6-trichloro-1,3,5-triazine with a mass concentration of 3%, stirred for 3 hours, filtered, and collected After filtering the residue, wash with 10 times the volume of the filter residue in water, in order to remove excess 2,4,6-trichloro-1,3,5-triazine.

[0026] Add 6mg of carbonic anhydrase per gram of modified bagasse, add the carbonic anhydrase (model: 9001-03-0) purchased from the market in the modified bagasse from the market; After oscillating for 12 hours at 60°C, put it in a freezer at 4±1°C for 24 hours, filter, and wash the filter residue repeatedly with 0.2mol / L phosphate buffer solution and distilled water until the enzyme...

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Abstract

The invention discloses a method for immobilizing carbonic anhydrase by use of modified bagasse. The method comprises the steps of putting bagasse into NaOH and soaking for 20-30 hours; neutralizing the pH of the solution to 7.0+ / -0.2 by use of HCl with mass concentration of 0.5-2%; filtering and drying; putting into a 2,4,6-trichloro-1,3,5-triazine solution with mass concentration of 0.5-3%, and stirring for 1-3 hours; filtering, collecting filter residues, and washing with water; adding 3-6mg of carbonic anhydrase into every gram of modified bagasse; shaking at a temperature of 30-60 DEG C for 1-12 hours; putting into a freezer at 4+ / -1 DEG C, and immobilizing for 12-24 hours; filtering and flushing. In the method disclosed by the invention, by adopting bagasse as a carrier of immobilization enzyme, the use of an expensive immobilization carrier can be reduced, the economic benefits of the bagasse can be increased, and the reaction conditions for wide application of the flue gas of a factory boiler are created.

Description

technical field [0001] The invention relates to a method for immobilizing carbonic anhydrase, in particular to a method for immobilizing carbonic anhydrase by using modified bagasse. The immobilized carbonic anhydrase involved in the present invention can be used for low concentration CO such as boiler flue gas 2 The absorption and enrichment belong to the fields of chemical modification and biotechnology. Background technique [0002] Carbonic anhydrase (CA) catalyzes CO 2 +H 2 O==HCO 3 - +H + the reversible reaction. The enzyme is ubiquitous in the animal and plant kingdoms. The first discovery was in animal bovine serum. Calcium bicarbonate catalyzes CO 2 The ability of hydration is very strong, and the reaction rate can reach 10 6 / s. Carbonic anhydrase (CA) accelerates CO capture from large combustors, study shows 2 potential. [0003] On the other hand, the flue gas emitted by industrial boilers after burning coal, crude oil, natural gas, waste fiber and o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02
Inventor 于淑娟黄凯扶雄张新林胡彪张平军
Owner SOUTH CHINA UNIV OF TECH
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