Rapid chemiluminiscence detection kit for quinolone drugs and testing method of kit

A chemiluminescence detection, quinolone technology, applied in the direction of testing food, material inspection products, etc., can solve the problems of poor reagent stability, difficulty in improving the degree of automation of operations, time-consuming and laborious, and achieve stable reagents, small errors, and reduce false positives. rate effect

Inactive Publication Date: 2014-03-12
LUOYANG LAIPSON INFORMATION TECH +1
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 1) Heterogeneous reaction: In order to separate free substances and conjugated substances during the detection process, a multi-step plate washing process is required, which is time-consuming and laborious, and it is difficult to improve the automation of the ope...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rapid chemiluminiscence detection kit for quinolone drugs and testing method of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The present invention will be further elaborated below in combination with specific embodiments.

[0035] A rapid chemiluminescence detection kit for quinolones, the kit is equipped with a luminescent plate, a quinolone polyclonal antibody treated with a chemical substrate, a goat anti-rabbit antibody labeled with horseradish peroxidase, and a quinolone A series of standard solutions, concentrated phosphate buffer solution, concentrated washing solution and luminescent solution, the luminescent plate has coated antigen, the coated antigen is obtained by coupling quinolone drug hapten and OVA by mixed anhydride method, the The quinolone polyclonal antibody treated with chemical substrates is obtained by immunizing New Zealand white rabbits with conjugates prepared by coupling norfloxacin, ofloxacin derivatives and OVA as immunogens;

[0036] The luminescence plate is a milky white opaque 96-well polystyrene chemiluminescence plate;

[0037] Described quinolone series st...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a rapid chemiluminiscence detection kit for quinolone drugs and a testing method of the kit. The kit is internally provided with a luminescent plate, a quinolones polyclonal antibody treated by a chemical primer, a goat-anti-rabbit antibody marked by horseradish peroxidase, a quinolones-series standard solution, a concentrated phosphate buffer, a concentrated washing solution and a luminescent solution, wherein the luminescent plate is provided with a coating antigen; the coating antigen is obtained by coupling a quinolone drugs dry hapten and OVA by adopting a mixed anhydride method; the quinolones polyclonal antibody treated by the chemical primer is obtained by taking a conjugate as an immunogen to immunize New Zealand white rabbits and the conjugate is prepared by coupling norfloxacin, an ofloxacin derivative and the OVA; the lowest detection limit of a chemiluminiscence enzyme-linked immunosorbent assay detection kit can be up to 0.1ng/ml, a linear detection range is 0.1ng/ml-8.1ng/ml, the in-plate variable coefficient is less than 20% and the recovery rate in a water sample ranges from 78% to 118%.

Description

technical field [0001] The invention relates to a direct chemiluminescence detection kit and a test method thereof, in particular to a rapid chemiluminescence detection kit and a test method for the residual amount of quinolones in samples such as animal tissues, eggs, and honey. Background technique [0002] Quinolones can be used to treat respiratory tract infection, genitourinary system infection, digestive system infection and other types of various infections, and can also be used for anti-tumor and anti-viral effects. In addition to being applied to the treatment of human diseases, it is also applied to the aquaculture industry. This type of drug is easy to induce bacterial resistance, so the management method for the use of food-producing animals is becoming more and more strict. Countries such as the European Union and North America only approve enrofloxacin as an antibacterial drug for animals, norfloxacin and ciprofloxacin Star has been banned for use in a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/02
Inventor 王善普王兴普刘欢闫玉良王宇东
Owner LUOYANG LAIPSON INFORMATION TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap