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Kit and method for detecting fibronectin concentration in human urine

A technology for fibronectin and human detection, applied in the field of bioengineering, can solve the problems of complex process and low accuracy, and achieve the effect of high accuracy and remarkable technological progress

Inactive Publication Date: 2014-03-19
上海北加生化试剂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above-mentioned defects existing in the prior art, the technical problem to be solved by the present invention is to provide a test kit for detecting the concentration of fibronectin in human urine and a method thereof. The concentration kit and its method should solve the technical problems of complex process and low accuracy in the determination of urinary fibronectin (FN) concentration by immunodiffusion method and ELISA double-antibody sandwich technology in the prior art

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  • Kit and method for detecting fibronectin concentration in human urine
  • Kit and method for detecting fibronectin concentration in human urine
  • Kit and method for detecting fibronectin concentration in human urine

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Embodiment 1

[0020] The invention provides a test kit for detecting the concentration of fibronectin in human urine, comprising two reagents, the I reagent consists of disodium hydrogen phosphate, potassium dihydrogen phosphate, PEG6000, EDTA-NA 2 And TX-100 composition, in described I reagent, the mass percentage concentration of described disodium hydrogen phosphate is 28.6g / L, the mass percentage concentration of described potassium dihydrogen phosphate is 2.7g / L, the described The mass percent concentration of PEG6000 is 50g / L, and the EDTA-NA 2 The mass percentage concentration is 1g / L, the volume concentration of the TX-100 is 0.2ml / L, and the II reagent consists of disodium hydrogen phosphate, potassium dihydrogen phosphate, PEG6000, EDTA-NA 2 , TX-100, fibronectin (FN) antiserum or fibronectin (FN) monoclonal antibody, in the II reagent, the mass percent concentration of disodium hydrogen phosphate is 28.6g / L, The mass percent concentration of the potassium dihydrogen phosphate is...

Embodiment 2

[0024] Embodiment 2 Immunotransmission nephelometric method

[0025] When a beam of incident light irradiates the IC in the liquid phase, part of the light is absorbed by the IC particles, part of the light passes through, and part of the light is scattered by the IC particles. Use a nephelometer to measure the ratio of the transmitted light intensity to the incident light intensity or the absorbance value in the direction of the light path of the light source (0°) to determine the amount of the antigen to be tested. This method is called turbidimetry.

[0026] 1.1 Applicable instruments

[0027] Semi-automatic and fully automatic biochemical analyzers.

[0028] 1.2 Analysis method

[0029] Immunoturbidimetry.

[0030] 1.3 Performance requirements

[0031] 1.3.1 Reagent Appearance

[0032] R1: colorless clear transparent liquid;

[0033] R2: Pale yellow clear liquid.

[0034] 1.3.2 Reagent blank absorbance (A)

[0035] Absorbance (A): R 1 +R 2 ≤0.07A (at a temperatur...

Embodiment 3

[0098] Embodiment 3 immunoscattering turbidimetry

[0099] When a beam of incident light irradiates the IC in the liquid phase, part of the light is absorbed by the IC particles, part of the light passes through, and part of the light is scattered by the IC particles. Measure the intensity of scattered light at a certain angle (such as 70°) of the light path of the light source. The electrical signal on the photocell is proportional to the intensity of scattered light, which is converted into the content of the tested antigen by a microcomputer. This method is called nephelometry.

[0100] 1.1 Applicable instruments

[0101] Semi-automatic and fully automatic biochemical analyzers.

[0102] 1.2 Analysis method

[0103] Immunoturbidimetry.

[0104] 1.3 Performance requirements

[0105] 1.3.1 Reagent Appearance

[0106] R1: colorless clear transparent liquid;

[0107] R2: Pale yellow clear liquid.

[0108] 1.3.2 Reagent blank absorbance (A)

[0109] Absorbance (A): R 1 ...

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Abstract

The invention belongs to the field of bioengineering and provides a kit for detecting fibronectin concentration in human urine. The problems that the method for measuring the fibronectin concentration is complex in steps, low in accuracy and low in repeatability by adopting an immunodiffusion method and an enzyme linked immunosorbent assay (ELISA) double-antibody sandwich technology are solved. The kit comprises two reagents, namely a reagent I consisting of a phosphate buffer solution, and a reagent II consisting of a phosphate buffer solution and goat (rabbit) anti-human fibronectin antiserum or fibronectin monoclonal antibody. The invention also provides a method for detecting the fibronectin concentration in the human urine by adopting the kit. The kit and the method are used for measuring the fibronectin concentration in urine, are simple and convenient in step, high in accuracy and high in repeatability and are used for automatic analyzers.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a detection kit, in particular to a reagent for detecting the concentration of fibronectin (FN) in human urine by immunoturbidimetry and turbidimetry box and its methods. Background technique [0002] Fibronectin, also known as fibronectin (FN for short), consists of two similar polypeptide chain subunits, and the molecular weight of the subunits is 220-250KD. In the prior art, the immunodiffusion method and ELISA double-antibody sandwich technology are used to measure the concentration of fibronectin (FN) in urine. The disadvantages are cumbersome operation, high work intensity, and long retention time. Generally, it takes 8-48 hours. The accuracy is poor. [0003] Because of the low concentration of fibronectin (FN) in urine, there have been no reports on the use of this kit and method at home and abroad. The kit and method for determining the concentration of fibro...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N33/54313G01N33/57407
Inventor 朱慧琳朱晓敏杨杰刘颖冰
Owner 上海北加生化试剂有限公司
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