Preparation method for effective components of buxus sinica varparvifolia and application of effective components

A technology of Buxus microphylla and active components, which is applied in the direction of medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas, can solve the problems of unknown drug efficacy material basis for cardiovascular diseases, and achieve improved operability and improved The effect of medicine

Active Publication Date: 2014-03-26
GUIYANG MEDICAL UNIVERSITY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It has been reported that the non-alkaloid components of Euonymus microphylla are mainly flavonoids (non-alkaloid chemical components in Buxus poplar, Yunnan Plant Research, 2006, 28(4):429-432), but its efficacy in treating cardiovascular diseases The material basis is unknown, and there are no relevant reports on alkaloid compounds, which are unique active ingredients in this genus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method for effective components of buxus sinica varparvifolia and application of effective components
  • Preparation method for effective components of buxus sinica varparvifolia and application of effective components
  • Preparation method for effective components of buxus sinica varparvifolia and application of effective components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 100 g of Buxus microphylla was pulverized and extracted twice with 10 times the mass concentration of 75% ethanol aqueous solution under reflux, and the time of each extraction was 2 hours. The extracts were combined, filtered, and concentrated under reduced pressure until there was no alcohol smell, and 400 ml aqueous solution of Buxus microphylla was obtained. .

[0039] The aqueous solution of Euonymus microphylla was acidified with aqueous sulfuric acid until the pH value of the acidified solution was 3, extracted three times with 450 ml of ethyl acetate, combined with the ethyl acetate extracts, concentrated under reduced pressure to the extract to obtain the ethyl acetate extract;

[0040] After ethyl acetate extraction, the water part was adjusted to pH 9 with sodium hydroxide solution, extracted three times with 450 ml of chloroform, the extracts were combined, concentrated under reduced pressure, and dried under normal pressure to obtain the chloroform extract, ...

Embodiment 2

[0044] After crushing 100g of Buxus microphylla, use 20 times the mass concentration of 95% ethanol aqueous solution to reflux and extract 4 times, each extraction time is 0.5 hours, combine the extracts, filter, concentrate under reduced pressure to recover the solvent, and obtain the extract of Buxus microphylla

[0045] Suspend the extract of Buxus microphylla with 150 mL of water, acidify the aqueous solution with aqueous hydrochloric acid until the pH of the acidified solution is 1, and extract once with 150 ml of ethyl acetate to obtain the water part after ethyl acetate extraction; The water part of the solution was adjusted to pH 14 with potassium hydroxide solution, extracted twice with 150 ml of chloroform, the extracts were combined, concentrated under reduced pressure, and dried under normal pressure to obtain the effective components of Buxus microphylla.

Embodiment 3

[0047]After crushing 100g of Euonymus microphylla, reflux extraction with 5 times the mass concentration of 50% ethanol aqueous solution for 3 times, each extraction time is 4 hours, combine the extracts, filter, concentrate under reduced pressure until there is no alcohol smell, and continue to concentrate to obtain 200 ml Buxus microphylla aqueous solution.

[0048] The aqueous solution of Euonymus microphylla is acidified with formic acid aqueous solution until the pH value of the acidified solution is 6, and extracted 5 times with 200 ml of ethyl acetate to obtain the water part after ethyl acetate extraction; the water part after ethyl acetate extraction is treated with calcium hydroxide The solution was adjusted to a pH value of 8, extracted four times with 200 ml of chloroform, the extracts were combined, concentrated under reduced pressure, and dried under normal pressure to obtain the effective components of Euonymus microphylla.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method for effective components of buxus sinica varparvifolia and application of the effective components and belongs to the field of extraction of the effective components of traditional Chinese medicines. The preparation method comprises the following steps of a, extracting the buxus sinica varparvifolia through 0-95% ethanol water to obtain an extracting solution; b, recycling a solvent in the extracting solution until the alcohol taste disappears to obtain a buxus sinica varparvifolia aqueous solution or extract, then acidifying the buxus sinica varparvifolia aqueous solution or extract through acidic water until the pH of the acidified aqueous solution is 1-6, extracting the aqueous solution through ethyl acetate to obtain a water part obtained by extraction of the ethyl acetate; C, alkalizing the water part through alkaline until the pH of the alkalized solution is 8-14, extracting the alkalized solution through chloroform, and concentrating and drying the chloroform extracting solution to obtain the effective components of the buxus sinica varparvifolia. The preparation method disclosed by the invention has the beneficial effects that due to the reasonable extracting, enriching and separating methods, the effective components of the buxus sinica varparvifolia, which has a better effect for protecting myocardial oxidative damage and can be used for preparing a myocardial oxidative damage resisting medicine, can be obtained.

Description

technical field [0001] The invention belongs to the field of extraction of effective components of traditional Chinese medicines, and in particular relates to a preparation method and application of effective components of Buxus microphylla. Background technique [0002] Boxwood powder has always been an effective drug for folk prescriptions to treat "heart disease". At the same time, boxwood alkaloids in Buxus plants have also shown significant improvement in heart function in patients with coronary heart disease in related studies (Huang Dandan, Xu Li. Huanwei Boxus Advances in research on the pharmacological effects of star D on the cardiovascular system [J]. Journal of Guiyang College of Traditional Chinese Medicine. 2013, 35 (1): 241-243). Buxus sinica (Rehd.Et Wils.) Cheng is an evergreen shrub or small tree plant belonging to the genus Buxus of the Buxaceae family (Buxaceae), also known as Qingming dwarf, millennium dwarf, yellow-headed mugwort, evergreen, Huangmu and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P9/10A61P9/00
Inventor 廖尚高王永林刘俊宏关焕玉张越浩钟瑞锋李勇军孙佳何迅兰燕宇
Owner GUIYANG MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap