Preparation method for coproducing high-purity galactooligosaccharide and galactitol

A technology of galacto-oligosaccharides and galactitol, which is applied in the field of preparation of high-purity galacto-oligosaccharides co-produced with galactitol, which can solve the problems of unfavorable large-scale industrial production, low utilization rate of raw and auxiliary materials, and imperfect production technology, etc. problems, to achieve the effect of low miscellaneous sugar content, low production cost and high product quality

Active Publication Date: 2014-03-26
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In China, the industrial production of galactooligosaccharides is still in its infancy, and the production process is still not perfect. Most of the products are type 30 and type 55. The purity of galactooligosaccharides is 30% and 55% respectively, and the product purity is relatively low. The purification process is mainly based on biological fermentation purification, the process is backward, the product quality is low, the utilization rate of raw and auxiliary materials is low, and the production cost is high, which is not conducive to large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take refined lactose, adjust the mass percentage of syrup to 30% with reverse osmosis water, raise the temperature to 38°C, adjust the pH value to 6.0, add β-galactosyltransferase at 0.8Kg / ton dry basis, enzymatic conversion for 24 hours, and detect low The polygalactose content is 35%.

[0028] Heat the syrup after the enzyme conversion to 70°C, add 1% (mass ratio of powdered activated carbon to hydrogenated dextrin dry basis) powdered activated carbon, stir evenly, keep for 30 minutes for decolorization, and then use a plate and frame filter press to filter the activated carbon. The filtered filtrate is subjected to ion exchange desalination.

[0029] The positive column of strong acid cation resin and the negative column of weak base anion resin respectively are treated and regenerated by conventional methods. Then, the decolorized and filtered syrup passes through the positive column-negative column-positive column at a flow rate of 3 times the resin volume / hour at...

Embodiment 2

[0041] Take refined lactose, use reverse osmosis water to adjust the mass percentage of syrup to 50%, raise the temperature to 50°C, adjust the pH value to 7.0, add β-galactosyltransferase at 1.5Kg / ton dry basis, enzymatic conversion for 24 hours, and detect low The polygalactose content is 37%.

[0042] Heat the syrup after the enzyme conversion to 80°C, add 1% (mass ratio of powdered activated carbon to hydrogenated dextrin dry basis) powdered activated carbon, stir evenly, keep for 30 minutes for decolorization, and then use a plate and frame filter press to filter the activated carbon. The filtered filtrate is subjected to ion exchange desalting.

[0043] The positive column of strong acid cation resin and the negative column of weak base anion resin respectively are treated and regenerated by conventional methods. Then, the decolorized and filtered syrup passes through the positive column-negative column-positive column at a flow rate of 3 times the resin volume / hour at ...

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Abstract

The invention relates to a preparation method for coproducing high-purity galactooligosaccharide and galactitol, which comprises the following steps: a) enabling galactosy transferase to act on a lactose solution, thus generating a galactooligosaccharide mixed saccharide solution; b) purifying galactooligosaccharide and miscellaneous saccharide components containing lactose, glucose and the like through a chromatographic separation technology, wherein the purity of the galactooligosaccharide is up to 90% or above; c) degrading the purified and separated miscellaneous saccharides by using lactase; d) by taking skeletal nickel as a catalyst, under the conditions that the temperature is 80-130 DEG C and the pressure is 8-12 Mpa, performing hydrogenation reaction on the enzyme-degraded miscellaneous saccharide components, thus preparing mixed saccharide alcohol; e) performing chromatographic separation and purification on the hydrogenated mixed saccharide alcohol solution, thus producing high-purity galactitol and miscellaneous alcohol components containing sorbitol and the like; and f) respectively performing activated carbon decoloring, ion exchange desalting and vacuum concentration on the galactooligosaccharide, galactitol and sorbitol after chromatographic separation, thus obtaining corresponding products.

Description

technical field [0001] The invention belongs to the technical field of preparation of functional oligosaccharides, and in particular relates to a method for preparing high-purity galactose-oligosaccharides co-produced with galactitol. Background technique [0002] Galactooligosaccharide is a functional oligosaccharide with natural properties, and its molecular structure is generally 1-7 galactosyl groups connected to galactose or glucose molecules. Galactooligosaccharides have good acid and heat stability, no dental caries, no digestion and absorption in the human body, low calorific value, superior physiological and functional characteristics, and can effectively proliferate bifidobacteria. It is an emerging functional oligosaccharide sugar. [0003] In China, the industrialized production of galactooligosaccharides is still in its infancy, and the production process is still not perfect. Most of the products are 30 and 55 types. The purity of galactooligosaccharides is 30...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P7/18
Inventor 干昭波邵先豹窦宝德窦光朋张安国贾玉秋
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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