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Detection method for simultaneously detecting nitrite, nitrate and polyphosphates in aquatic products

A nitrite and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that nitrate, nitrite and polyphosphate cannot be measured at the same time, different phosphate cannot be quantified, and the sensitivity is not high. , to achieve the effect of ensuring food safety, low detection limit and improving extraction efficiency

Active Publication Date: 2014-03-26
舟山出入境检验检疫局综合技术服务中心
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Problems solved by technology

but cannot quantify the different phosphates
Since the organism itself contains a certain amount of phosphoric acid, this method still cannot simultaneously determine nitrate, nitrite and polyphosphate
The determination of pollutants and additives in aquatic products by ion chromatography is a research hotspot at present, but a rapid ultrasonic-assisted extraction-solid phase extraction-ion chromatography (UAE-SPE-IC) has not yet been established for the simultaneous detection of nitric acid in aquatic products Methods for the detection of salt, nitrite and polyphosphate
The relevant invention patents that have been declared in China have application number 201210014139.2 and a method for detecting nitrite and nitrate in sour pickled vegetables disclosed on application number 201210014139.2 and application date 2012-01-18, but the sensitivity of these methods is not high in actual detection. Simultaneous detection of nitrate, nitrite and polyphosphate in aquatic products is difficult for rapid detection application

Method used

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  • Detection method for simultaneously detecting nitrite, nitrate and polyphosphates in aquatic products
  • Detection method for simultaneously detecting nitrite, nitrate and polyphosphates in aquatic products
  • Detection method for simultaneously detecting nitrite, nitrate and polyphosphates in aquatic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Ion Chromatography Simultaneous Determination of Nitrite, Nitrate and Polyphosphate in Fish Meat

[0043] 1. Sample pretreatment:

[0044] Fully mix and crush the fish sample to be tested, put it into a container, seal it, and refrigerate (-18°C) for later use;

[0045] 2. Rapid ultrasound-assisted extraction (UAE):

[0046] Weigh 5g (accurate to 0.1g) of the crushed sample into a 150mL Erlenmeyer flask, add 50mL of deionized water, put it in an ultrasonic oscillator for 30min after homogenization, and shake once every 5min to keep the solid phase completely dispersion. Then centrifuge at 7000r / min for 10min, filter, take 20mL of the filtrate, add 3mL of 20% trifluoroacetic acid solution to mix, store in a refrigerator at 0-4°C for 30 minutes, and then centrifuge in a centrifuge at 8000rpm for 10 minutes.

[0047] 3. Solid phase extraction (SPE):

[0048] Take SPE solid phase extraction C 18 The column was first activated with 5mL of methanol and then was...

Embodiment 2

[0062] Example 2 Ion Chromatography Simultaneous Determination of Nitrite, Nitrate and Polyphosphate in Dry Products

[0063] The specific scheme is the same as that in Example 1, except that the sample weight is 2.5 g (accurate to 0.1 g). According to the measurement results, the average recovery rate of standard addition was above 75.3%, meeting the detection requirements.

Embodiment 3

[0064] Embodiment 3 utilizes ion chromatography to monitor the content of nitrite, nitrate and polyphosphate in aquatic products

[0065] The samples were randomly purchased from aquatic product processing plants and circulation markets, and there were 10 batches of samples in total. The instruments and reagents used were as described above.

[0066] The sample pretreatment method, ion chromatographic parameters and preparation of the standard curve are the same as in Example 1.

[0067] Each batch of samples was quality controlled and assured using the same fish sample control (orthophosphate and nitrate only) and sample spike recovery assay. Precisely weigh 5.0g of fish meat sample, add 50mL of deionized water, add standard substance quantitatively after homogenization, and pre-process and put the sample on the machine at the same time after mixing.

[0068] The results show that the test results of the negative control samples only contain orthophosphate and nitrate, and t...

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Abstract

The invention relates to a method for detecting multiple additives and pollutants and in particular relates to a detection method for simultaneously detecting nitrite, nitrate and polyphosphates in aquatic products. The nitrate, nitrite and polyphosphates in the aquatic products are simultaneously detected by adopting rapid ultrasound-assisted extraction-solid-phase extraction-ion chromatography (UAE-SPE-IC), the recovery rate of the nitrate, nitrite and polyphosphates is greatly improved through rapid ultrasound-assisted extraction, the interference of other substances is effectively removed by combining with a C18 column, the sensitivity of actual detection is improved, the ion chromatography (IC) has high separation capacity on substances with high electrolytic coefficient, such as anion and the analysis in different valence states can be performed, the detection limit is low, and the method is suitable for quantitative detection of the nitrite, nitrate and polyphosphates in aquatic products and food safety inspection.

Description

technical field [0001] The invention relates to a method for detecting various inorganic salts, in particular to a method for simultaneously detecting nitrite, nitrate and polyphosphate in aquatic products. Background technique [0002] Nitrate in seafood will be reduced to nitrite under the action of bacteria and enzymes. Some seafood that has been stored for a long time, has a tendency to spoil and has a high protein content has a higher content of nitrous acid. Nitrite can reduce the oxygen-carrying capacity of the blood, leading to methemoglobinemia. It can combine with amines to form nitrosamines, which can induce canceration of the digestive system. Incidents of nitrite poisoning have also been reported from time to time. In order to control the human body's intake of nitric acid and nitrous acid, nitric acid and nitrous acid are listed as important indicators in food hygiene standards, and my country has put forward clear limit standards for nitric acid and nitrite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 邵宏宏
Owner 舟山出入境检验检疫局综合技术服务中心
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