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Composition and method for killing aspergillus fumigatus

A technology of Aspergillus fumigatus and bacterial strains, which is applied in the field of Aspergillus fumigatus biofilm and composition for killing Aspergillus fumigatus, and can solve the problems of Aspergillus fumigatus drug resistance and poor effect

Inactive Publication Date: 2014-04-02
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the commonly used antifungal drugs for the treatment of invasive aspergillosis include voriconazole, the first choice drug, which has good antifungal effect on Aspergillus fumigatus mycelial cells without extracellular matrix, but the effect on Aspergillus fumigatus mycelial cells in biofilm is not good. How to solve the problem of Aspergillus fumigatus resistance caused by biofilm has become a major problem in the successful treatment of invasive aspergillosis

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  • Composition and method for killing aspergillus fumigatus
  • Composition and method for killing aspergillus fumigatus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1.1 Materials

[0033] 1.1.1 Antifungal drugs Chlorogenic acid (CRA, National Institute for Food and Drug Control, batch number 110753-201314), voriconazole (VRC, National Institute for Food and Drug Control, batch number 100862-200701). All were dissolved in 100% dimethyl sulfoxide, prepared into storage solutions with concentrations of 102400 μg / ml and 1280 μg / ml respectively, and stored at -20°C after aliquoting.

[0034]1.1.2 Strains From September 2012 to May 2013, 30 Aspergillus fumigatus strains were collected in the Bacteria Laboratory of the First Affiliated Hospital of Guangxi Medical University. The strain with the strongest biofilm formation ability was selected by the crystal violet method as the subject Test strains. The quality control strain was Candida parapsilosis ATCC22019, which was donated to the Bacteria Laboratory of the First Affiliated Hospital of Guangxi Medical University.

[0035] 1.1.3 Carrier Glass cell slides, 13 mm in diameter (Haimen S...

Embodiment 2

[0049] According to the implementation of embodiment 1, the result that draws, table 1 is that XTT reduces method and measures metabolic activity, the XTT% of each group

[0050] group Early Aspergillus fumigatus biofilm mature Aspergillus fumigatus biofilm VRC group 71.07 85.29 512μg / ml CRA group 99.97 98.02 1024μg / ml CRA group 99.12 98.68 512μg / ml CRA group + VRC 17.82 35.89 1024μg / ml CRA group + VRC 10.23 19.21

[0051] Fluorescent staining and observation of fluorescent dead and live bacteria stained with laser confocal microscope, whether it is early or mature chlorogenic acid intervention group compared with the blank group, there is no significant difference, the hyphae are also dense and all are vibrant green; There was no significant difference in the mycelial density between the voriconazole group and the blank group, and a small number of mycelia showed inactive red and yellow with poor vitality. When acting on the...

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Abstract

The invention provides a composition and a method for killing aspergillus fumigatus, and belongs to the technical field of biology. The method for killing aspergillus fumigatus comprises the following steps: preparing a medicine; collecting a strain; preparing a vector and a spore suspension; performing a medicine sensitivity test; preparing an aspergillus fumigatus bio-membrane vector; acting the medicine to the aspergillus fumigatus bio-membrane; detecting the metabolic activity by virtue of an XTT (2,3bis(2methoxy4nitro5sulfophenyl)5[(phenylamino)carbonyl]2htetrazolium hydroxide) reduction method; preparing a fluorescent dye liquor, and performing fluorescent dyeing and observation; combining chlorogenic acid and voriconazole to kill aspergillus fumigatus. According to the composition and the method for killing aspergillus fumigatus, aspergillus fumigatus in bio-membranes can be killed, and the problem that an antifungal drug cannot kill aspergillus fumigatus in a bio-membrane can be solved.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to aspergillus fumigatus biofilm, in particular to a composition for killing aspergillus fumigatus and a method for killing aspergillus fumigatus. Background technique [0002] Aspergillus fumigatus is an opportunistic pathogen that can cause fatal infections in immunocompromised patients. The invasive aspergillosis caused by it is one of the main causes of death in immunocompromised patients, with a mortality rate of 30-100%. At present, azole antifungal drugs are the main drugs for the treatment of invasive aspergillosis. Among them, voriconazole has become the drug of choice for the treatment of invasive aspergillosis due to its stronger antibacterial ability and fewer adverse reactions. However, although it has been used in the treatment of The mortality rate of invasive aspergillosis has not been significantly reduced for many years, and the resistance rate of azole antifunga...

Claims

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Application Information

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IPC IPC(8): A61K31/506A61P31/10A61K31/216
Inventor 陈一强黄宏孔晋亮
Owner GUANGXI MEDICAL UNIVERSITY
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