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Rapid test method for adulteration of Chinese date honey

A technology for rapid determination of jujube nectar, applied in the preparation of test samples, measurement of color/spectral characteristics, etc., can solve the problems of expensive liquid chromatography-mass spectrometry instruments, limited applications, harsh operating conditions, etc., and achieve low cost of determination, The effect of wide applicability and low price

Inactive Publication Date: 2014-04-02
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that these two methods are only effective for the adulteration of rice syrup, but not for other adulteration methods, and the liquid chromatography-mass spectrometry instrument is expensive and the operating conditions are harsh, which limits the application of these two methods

Method used

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  • Rapid test method for adulteration of Chinese date honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Determination of self-made caramel adulterated jujube nectar

[0049] Weighed 50 g of natural jujube nectar, added 50 g of caramel (i.e. adulterated honey), and mixed well to prepare self-made caramel adulterated jujube nectar with 50% natural jujube nectar.

[0050] (1) Preparation of sample solution of adulterated jujube nectar with self-made caramel sugar: 1) Extraction of total alkaloids in self-made caramel-adulterated jujube nectar: ​​Weigh 50 g of the above-mentioned self-made caramel-adulterated jujube nectar, add 50 g of water, and mix well. Adjust the pH to 3 with 0.2 mol / L hydrochloric acid, extract with 60 ml of chloroform, separate the layers, and discard the organic phase. Adjust the pH to 10 with 0.2 mol / L sodium hydroxide aqueous solution. Add 100 ml of chloroform and shake fully for 10 min, separate the layers, discard the water layer, and evaporate the chloroform layer to dryness in a water bath to obtain the total alkaloid extract. 2) The...

Embodiment 2

[0055] Embodiment 2: Determination of self-made rice syrup adulterated jujube nectar

[0056] Weighed 70 g of natural jujube nectar, added 30 g of rice syrup (i.e. honey adulterant), and mixed well to prepare a self-made rice syrup adulterated jujube nectar with a natural jujube nectar content of 70%.

[0057] (1) Preparation of sample solution of self-made rice syrup adulterated jujube nectar: ​​1) Extraction of total alkaloids in self-made rice syrup adulterated jujube nectar: ​​Weigh 50 g of the above-mentioned self-made rice syrup adulterated jujube nectar, add 60 g of water, mix uniform. Adjust the pH to 4 with 0.6 mol / L hydrochloric acid, extract with 120 ml of chloroform, separate the layers, and discard the organic phase. Adjust the pH to 10 with 0.6 mol / L sodium hydroxide aqueous solution. Add 40 ml of chloroform to fully shake for 20 min, separate the liquid, discard the water layer, and evaporate the chloroform layer to dryness with a rotary evaporator to obtain t...

Embodiment 3

[0062] Embodiment 3: Determination of self-made thickener and pigment adulterated jujube nectar

[0063] Weigh 1.2 g of starch and moisten it with a small amount of water. Measure 200 mL of water, heat to a slight boil, pour in the wet starch, boil for 1 minute, cool to room temperature, and prepare a starch paste. Weigh 0.1 g of caramel pigment, add it to the starch paste, and mix well to prepare the dyed starch paste.

[0064] Weigh 40 g of natural jujube nectar, add 60 g of dyed starch paste (i.e. honey adulterant), and mix well to prepare a self-made thickener and pigment-adulterated jujube nectar with a content of 40% natural jujube nectar.

[0065] (1) Preparation of sample solution of self-made thickener and pigment-adulterated jujube nectar: ​​1) Extraction of total alkaloids in self-made thickener and pigment-adulterated jujube nectar: ​​Weigh 50 g of the above-mentioned self-made thickener and pigment-adulterated Jujube honey, add 50 g of water, and mix well. Adju...

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Abstract

The invention discloses a rapid test method for adulteration of Chinese date honey, and relates to the technical field of ultraviolet spectrophotometry detection. The rapid test method uses absorbance of total alkaloids in the Chinese date honey as a measurement index and comprises the following steps: (1) preparation of a Chinese date honey sample solution : 1) extracting the total alkaloids from the Chinese date honey sample, and 2) dissolving; (2) preparation of a reference solution; (3) determination of absorbance: using an ultraviolet spectrophotometer to determine the absorbance of the Chinese date honey sample solution and the reference solution at the maximum absorption wavelength place of the Chinese date honey sample solution; (4) drawing of working curves and computing of regression equations; (5) computing of the adulteration amount of Chinese date honey sample: according to the regression equations in the step (4) and the absorbance of the Chinese date honey sample solution in the step (3), computing the natural Chinese date honey content in the Chinese date honey sample to obtain the adulteration amount of the Chinese date honey sample. The method is wide in applicability, simple to operate, cheap in the equipment price, low in the determination cost, and suitable for popularization and application.

Description

technical field [0001] The invention relates to the technical field of detection by ultraviolet spectrophotometry. Background technique [0002] Honey is a nutritious natural food. The national food safety standard GB14963-2011 "Honey" defines honey as a natural sweet substance that is fully brewed after collecting nectar, secretion or honeydew of plants, mixed with its own secretions. Because the health care effect of honey is more and more recognized by more people, the consumer's demand for honey is increasing. Driven by exploitation, unscrupulous traders began to manufacture and sell fake products, and adulterated honey began to flood the market. [0003] The purpose of honey adulteration is to gain weight by adding cheap raw materials to natural honey. Such adulterants for weight gain are generally rice syrup, fructose syrup and maltose (commonly known as syrup). In order to achieve the effect of real ones, thickeners, such as starch paste or cellulose, as well as p...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/28
Inventor 高磊红刘宝友
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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