Rapid test method for adulteration of Chinese date honey

A technology for rapid determination of jujube nectar, applied in the preparation of test samples, measurement of color/spectral characteristics, etc., can solve the problems of expensive liquid chromatography-mass spectrometry instruments, limited applications, harsh operating conditions, etc., and achieve low cost of determination, The effect of wide applicability and low price

Inactive Publication Date: 2014-04-02
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

The disadvantage is that these two methods are only effective for the adulteration of rice syrup, but not for other adulteration methods, and the liq

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  • Rapid test method for adulteration of Chinese date honey
  • Rapid test method for adulteration of Chinese date honey

Examples

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Example Embodiment

[0048] Example 1: Determination of homemade malt sugar adulterated jujube nectar

[0049] Weigh 50 g of natural jujube nectar, add 50 g of maltose (i.e. honey adulterate), and mix well to prepare homemade maltose adulterated jujube nectar with 50% natural jujube nectar.

[0050] (1) Preparation of self-made maltose adulterated jujube nectar sample solution: 1) Extraction of total alkaloids from self-made maltose adulterated jujube nectar: ​​Weigh 50 g of the above-mentioned self-made maltose adulterated jujube nectar, add 50 g of water, and mix well. Adjust the pH to 3 with 0.2 mol / L hydrochloric acid, extract with 60 ml chloroform, separate the liquids, and discard the organic phase. Adjust the pH to 10 with 0.2 mol / L sodium hydroxide aqueous solution. Add 100 ml of chloroform to fully shake for 10 min, separate the liquids, discard the water layer, and evaporate the chloroform layer to dryness in a water bath to obtain the total alkaloid extract. 2) Dissolve the above total alk...

Example Embodiment

[0055] Example 2: Determination of homemade rice syrup adulterated with jujube nectar

[0056] Weigh 70 g of natural date nectar, add 30 g of rice syrup (i.e. honey adulterant), and mix well to prepare a homemade rice syrup adulterated date nectar with 70% natural date nectar.

[0057] (1) Preparation of sample solution of self-made rice syrup adulterated jujube nectar: ​​1) Extraction of total alkaloids from self-made rice syrup adulterated jujube nectar: ​​Weigh 50 g of the above-mentioned self-made rice syrup adulterated jujube nectar, add 60 g of water, and mix uniform. Adjust the pH to 4 with 0.6 mol / L hydrochloric acid, extract with 120 ml chloroform, separate the liquids, and discard the organic phase. Adjust the pH to 10 with 0.6 mol / L sodium hydroxide aqueous solution. Add 40 ml of chloroform and shake well for 20 min, separate liquids, discard the water layer, and evaporate the chloroform layer to dryness by a rotary evaporator to obtain the total alkaloid extract. 2) ...

Example Embodiment

[0062] Example 3: Determination of self-made thickener and pigment adulterated jujube nectar

[0063] Weigh 1.2 g starch and moisten it with a small amount of water. Measure 200 mL of water, heat it to a slight boiling point, pour in the wet starch, boil slightly for 1 minute, and cool to room temperature to prepare a starch paste. Weigh 0.1 g of caramel pigment, add it to the above-mentioned starch paste, and mix well to obtain a dyed starch paste.

[0064] Weigh 40 g of natural jujube nectar, add 60 g of dyed starch paste (i.e. honey adulterant) and mix well to prepare a self-made thickener and pigment adulterated jujube nectar with 40% natural date nectar.

[0065] (1) Preparation of homemade thickener and pigment adulterated jujube nectar sample solution: 1) Homemade thickener and pigment adulterated jujube nectar extraction of total alkaloids: Weigh 50 g of the above homemade thickener and pigment adulterated For jujube nectar, add 50 g of water and mix well. Adjust the pH to...

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Abstract

The invention discloses a rapid test method for adulteration of Chinese date honey, and relates to the technical field of ultraviolet spectrophotometry detection. The rapid test method uses absorbance of total alkaloids in the Chinese date honey as a measurement index and comprises the following steps: (1) preparation of a Chinese date honey sample solution : 1) extracting the total alkaloids from the Chinese date honey sample, and 2) dissolving; (2) preparation of a reference solution; (3) determination of absorbance: using an ultraviolet spectrophotometer to determine the absorbance of the Chinese date honey sample solution and the reference solution at the maximum absorption wavelength place of the Chinese date honey sample solution; (4) drawing of working curves and computing of regression equations; (5) computing of the adulteration amount of Chinese date honey sample: according to the regression equations in the step (4) and the absorbance of the Chinese date honey sample solution in the step (3), computing the natural Chinese date honey content in the Chinese date honey sample to obtain the adulteration amount of the Chinese date honey sample. The method is wide in applicability, simple to operate, cheap in the equipment price, low in the determination cost, and suitable for popularization and application.

Description

technical field [0001] The invention relates to the technical field of detection by ultraviolet spectrophotometry. Background technique [0002] Honey is a nutritious natural food. The national food safety standard GB14963-2011 "Honey" defines honey as a natural sweet substance that is fully brewed after collecting nectar, secretion or honeydew of plants, mixed with its own secretions. Because the health care effect of honey is more and more recognized by more people, the consumer's demand for honey is increasing. Driven by exploitation, unscrupulous traders began to manufacture and sell fake products, and adulterated honey began to flood the market. [0003] The purpose of honey adulteration is to gain weight by adding cheap raw materials to natural honey. Such adulterants for weight gain are generally rice syrup, fructose syrup and maltose (commonly known as syrup). In order to achieve the effect of real ones, thickeners, such as starch paste or cellulose, as well as p...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/28
Inventor 高磊红刘宝友
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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