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Technique for preparing citric acid by fermenting beta-cyclodextrin mother solution

A technology of cyclodextrin and citric acid, applied in the direction of fermentation, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of high energy consumption, energy waste, and high temperature of fermentation liquid, and achieve low energy consumption and high speed , to achieve the effect of efficient utilization

Active Publication Date: 2014-04-09
山东英轩实业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The thermal sterilization technology commonly used now, although the sterilization efficiency is higher and the effect is better, but the energy consumption is high, and the temperature of the fermentation liquid after sterilization is high, resulting in unnecessary waste of energy

Method used

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  • Technique for preparing citric acid by fermenting beta-cyclodextrin mother solution

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Corn is crushed, sieved, pulped, and liquefied by two consecutive steam jets to obtain a corn liquefaction liquid with a total sugar content of 20%, which is subjected to plate and frame filtration to obtain a liquefied clear liquid with a total sugar content of 18%.

[0029] After mixing the β-cyclodextrin mother liquor with a total sugar content of 13% and the corn liquefaction solution at a ratio of 1:2, the total sugar content is 17.7%, measure the carbon-nitrogen ratio, add a small amount of ammonium sulfate, measure its pH, and calculate the added hydrogen The amount of sodium oxide solution, adjust the pH to 6.0, as the seed medium, add it to the citric acid seed culture tank, after high-temperature sterilization, inoculate the Aspergillus niger spore suspension, stir and ventilate, control the temperature at 36 ° C, and cultivate for 20 hours to obtain black Aspergillus fermented strains.

[0030] Mix the β-cyclodextrin crystallization mother liquor with a total...

Embodiment 2

[0034] The corn is crushed, sieved, pulped, sprayed and liquefied to obtain a corn liquefied liquid with a total sugar content of 19%, which is subjected to plate and frame filtration to obtain a liquefied clear liquid with a total sugar content of 17%.

[0035] After mixing the β-cyclodextrin mother liquor with a total sugar content of 14% and the corn liquefaction liquid at a volume ratio of 1:3, the total sugar content is 17.8%, measure the carbon-nitrogen ratio, add a small amount of ammonium nitrate, measure its pH, and calculate the added hydrogen The amount of potassium oxide solution, adjust the pH to 6.5, as the seed medium, add it to the citric acid seed culture tank, after high-temperature sterilization, inoculate the Aspergillus niger spore suspension, stir and ventilate, control the temperature at 34 ° C, and cultivate for 25 hours to obtain black Aspergillus fermented strains.

[0036] Mix the β-cyclodextrin crystallization mother liquor with a total sugar conten...

Embodiment 3

[0040] The corn is crushed, sieved, pulped, and liquefied by two consecutive steam injections to obtain a corn liquefied liquid with a total sugar content of 22%, which is subjected to plate and frame filtration to obtain a liquefied clear liquid with a total sugar content of 20%.

[0041] After mixing the β-cyclodextrin mother liquor with a total sugar content of 14% and the corn liquefaction liquid at a ratio of 1:2, the total sugar content is 19.3%, measure the carbon-nitrogen ratio, add a small amount of ammonium nitrate, measure its pH, and calculate the added hydrogen The amount of potassium oxide solution, adjust the pH to 6.8. As a seed medium, add it to a citric acid seed culture tank, perform high-temperature sterilization, inoculate the Aspergillus niger spore suspension, stir and ventilate, control the temperature at 37° C., and cultivate for 30 hours to obtain Aspergillus niger fermentation strains.

[0042] Mix the β-cyclodextrin crystallization mother liquor with ...

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Abstract

The invention provides a technique for preparing citric acid by fermenting a beta-cyclodextrin mother solution, which comprises the following steps: proportionally mixing a beta-cyclodextrin mother solution with a corn liquefied solution, adding a right amount of alkali liquor to regulate the pH value to 6.0-6.8 to obtain a seed culture medium, sterilizing, inoculating an Aspergillus niger spore suspension, and culturing while ventilating to supply oxygen at controlled temperature to obtain a Aspergillus niger fermentation strain; and adding a right amount of corn liquefied clear solution into a beta-cyclodextrin crystallization mother solution to enhance the total sugar content to 15-17%, adding a right amount of alkali liquor to regulate the pH value to 6.0-6.8, adding a right amount of inorganic nitrogenous substance to regulate the carbon-nitrogen ratio so as to obtain a fermentation culture medium, sterilizing, inoculating the Aspergillus niger fermentation strain, and fermenting while ventilating to supply oxygen at the controlled temperature of 34-37 DEG C, thereby obtaining the citric acid fermentation solution. The technique implements high-efficiency utilization of the beta-cyclodextrin mother solution, and provides an excellent raw material source for citric acid fermentation production.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a process for preparing citric acid by fermenting beta-cyclodextrin mother liquor. Background technique [0002] Now β-cyclodextrin is generally produced enzymatically using starch as a substrate. During its production process, after the starch hydrolyzate is concentrated and cooled, the β-cyclodextrin crystallizes out. The resulting crystallization mother liquor still contains a relatively high content of starch hydrolyzate, including α-cyclodextrin, γ-cyclodextrin and other carbohydrates. If it is directly discharged as waste liquid, it will not only waste resources, but also increase the burden of sewage treatment. Therefore, it should be used rationally. At present, its utilization is mainly for the extraction and preparation of α-cyclodextrin and γ-cyclodextrin. If it is used to prepare fermentation medium, the carbohydrates therein can be fully utilized by microor...

Claims

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Application Information

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IPC IPC(8): C12P7/48C12R1/685
Inventor 李俭李世勇马钦元于明华陈金凤
Owner 山东英轩实业股份有限公司
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