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Extracorporal test kit for TAFI (Thrombin Activatable Fibrinolysis Inhibitor) content, and test method of extracorporal test kit

An in vitro detection and kit technology, which is applied in measurement devices, instruments, disease diagnosis, etc., can solve the problems of expensive equipment, long detection time, and low detection limit, and achieves simple operation, short detection time, and low detection limit. Effect

Active Publication Date: 2014-04-16
LIAONING MEDI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a kind of in vitro detection method of TAFI content, and it has solved the defects such as expensive equipment that exists in the prior art, detection time is longer, compared with other detection methods, has simple and convenient operation, fast, low cost, diagnosis Sensitivity, low detection limit, etc., as an auxiliary diagnosis for clinical prediction of cardiovascular and cerebrovascular diseases, can significantly improve the detection rate and accuracy of the disease, which is conducive to early detection and early treatment of the disease; reduce unnecessary Pain and medical expenses, improve the quality of life of the examinee

Method used

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  • Extracorporal test kit for TAFI (Thrombin Activatable Fibrinolysis Inhibitor) content, and test method of extracorporal test kit
  • Extracorporal test kit for TAFI (Thrombin Activatable Fibrinolysis Inhibitor) content, and test method of extracorporal test kit

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preparation example Construction

[0031] 1. Preparation of gold nanoparticles

[0032] The nano-gold used in the present invention is prepared by using sodium citrate and chloroauric acid. Firstly, the chloroauric acid solution is added with a small amount of nano-gold protective agent, then heated to boiling with an electric furnace, and trisodium citrate solution is added according to the mass ratio of 4:5. After continuing to heat for 10min, the existing nano-gold solution is calculated, and the volume of the nano-gold solution is theoretically prepared. After constant volume, utilize a nucleic acid protein analyzer or an ultraviolet spectrophotometer to carry out full-wavelength scanning to determine its peak value. The nano-gold solution used in the present invention The solution is scanned at full wavelength, and its peak is between 515-530nm.

[0033] 2. Preparation of monoclonal antibody and gold-labeled antibody

[0034] Monoclonal antibodies and gold-labeled antibodies are mouse anti-human monoclona...

Embodiment 1

[0051] Taking human plasma as an example, the method of the present invention is used to measure the TAFI content of 60 patients' plasma samples diagnosed as myocardial infarction and 300 healthy plasma samples. The specific operation steps are as follows:

[0052] Step 1 Collect human individual biological samples and immediately centrifuge at 4°C within 30 minutes to take the supernatant as the sample to be tested, and put it in an ice box for use within 1 hour or store it at -20°C for later use;

[0053] Step 2 uses an in vitro detection kit to measure the TAFI content of the sample to be tested;

[0054] The contents of the in vitro detection kit include: TAFI biochemical detection reagent R, TAFI standard substance, TAFI quality control substance, and instructions for use.

[0055] Step 3 Take out the reagent R and place it at room temperature until the temperature is balanced;

[0056] Step 4 put the R reagent and TAFI standard in the corresponding position of the bioc...

Embodiment 2

[0061] The method of the invention can also measure the TAFI content in human urine. Collect human morning urine, centrifuge at 1500 rpm for 5 minutes at 4°C within 30 minutes, and take the supernatant as the sample to be tested. Subsequent steps are the same as in Embodiment 1.

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Abstract

The invention relates to an extracorporal test kit for TAFI content, and a test method of the extracorporal test kit. The extracorporal test kit for the TAFI content consists of a reagent, a TAFI standard product and a TAFI quality control product, wherein the reagent contains a monoclonal antibody nano-gold marker R that is specifically bonded with TAFI; the TAFI standard product and TAFI quality control product are used for making a standard curve for calculating the content of a TAFI antigen in a to-be-tested sample. The biological characteristic that nano-gold is bonded with an antibody, and a conventional biochemical test method is combined, so that the extracorporal test method of the extracorporal test kit is used for an extracorporal test of the content of the TAFI, a new extracorporal diagnostic method is developed for the TAFI, and the sensitivity of the extracorporal test of the TAFI is improved. According to the extracorporal test method, the problem that the equipment is expensive in the prior art is solved, the detectable rate and the accuracy rate on relevant diseases are improved, unnecessary pains and medical cost of a tested person are reduced, and the living quality of the tested person is improved.

Description

technical field [0001] The invention relates to a new method for detecting the content of thrombin-activatable fibrinolysis inhibitor (TAFI) for clinical prediction of the possibility of occurrence of cardiovascular and cerebrovascular diseases, in particular to an in vitro method for measuring the content of TAFI. Detection kit and detection method thereof. Background technique [0002] As we all know, thrombotic disease is a kind of disease that seriously affects health, especially cardiovascular and cerebrovascular embolism disease has become the first cause of death in our country. Since the clinical manifestations of thrombotic diseases vary widely, the diagnosis and treatment are often very complicated. Therefore, early detection and early treatment are particularly important. At present, the examination methods for thrombotic diseases mainly include electrocardiography, echocardiography, chest X-ray, blood pressure monitoring, cranial X-ray, brain CT scan, brain MRI ...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577G01N33/587G01N33/6803G01N2800/22G01N2800/224G01N2800/32G01N2800/7095
Inventor 李文欣刘峰
Owner LIAONING MEDI BIOTECH CO LTD
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