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Composition containing soy protein hydrolyzate

A technology of soybean protein and composition, which is applied in the direction of drug combination, peptide/protein composition, medical preparations containing active ingredients, etc., can solve the problems of reducing the proliferation ability of keratinocytes, poor skin health status, and deterioration of metabolism, etc., to achieve Prevention/improvement of sagging or wrinkles, improvement of decreased metabolism, improvement of wrinkles and sagging effects

Inactive Publication Date: 2014-04-23
ROHTO PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, if the adhesion between the basement membrane and epidermal keratinocytes is gradually weakened due to ultraviolet rays or aging, etc., it will not only cause sagging (for example, loosening of pores) or wrinkles, but also cause keratinocytes to be unable to adhere well. Decreases the proliferative ability of keratinocytes, and thus, the metabolism becomes poor, causing spots (deposition of melanin) or dullness
Furthermore, if a looseness occurs between such a basement membrane and keratinocytes, the supply of nutrients or the movement of growth factors from the dermis, which plays an important role as the basement membrane, to the epidermis will deteriorate, and the overall health of the skin will be poor, and there may be a thinning of the epidermis

Method used

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  • Composition containing soy protein hydrolyzate
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  • Composition containing soy protein hydrolyzate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Preparation of hydrolyzate of soybean protein (1)

[0075] Soybean protein was suspended so that the concentration of defatted soybean powder (trade name PROPHAM974, manufactured by ADM FAR EAST Co., Ltd.) was 5% (w / v), and added so that it became 0.05% (w / v) Pepsibio 1:10,000 (manufactured by Japan Biocon Co., Ltd.), which is a kind of pepsin, was decomposed at 50° C. for 18 hours. The pH of the reaction solution was implemented at pH 3.5 which is near the optimum pH of the protease.

[0076] After completion of the reaction, the above-mentioned reactant was heat-treated at 85°C for 15 minutes to inactivate the enzyme contained in the reactant, and then neutralized with 10N sodium hydroxide. Next, diatomaceous earth (Fuso Chemical, RADIOLITE #200) was precoated on the Buchner funnel, and suction filtration was performed. Next, the filtrate was concentrated under reduced pressure and freeze-dried to obtain powdered soybean protein hydrolyzate in a yield of ...

Embodiment 2

[0077] Example 2: Preparation of hydrolyzate of soybean protein (2)

[0078]Soybean protein was suspended so that defatted soybean powder (trade name PROPHAM974, manufactured by ADM FAR EAST Co., Ltd.) had a concentration of 5% (w / v), and pancreatic For protease, the decomposition reaction was carried out at 50°C for 18 hours. The pH of the reaction solution was implemented at pH 7.5, which is near the optimum pH of the protease.

[0079] After completion of the reaction, the above-mentioned reactant was heat-treated at 85° C. for 15 minutes to inactivate the enzyme contained in the reactant. Next, diatomaceous earth (Fuso Chemical, RADIOLITE #200) was precoated on the Buchner funnel, and suction filtration was performed. Next, the filtrate was concentrated under reduced pressure and freeze-dried to obtain powdered soybean protein hydrolyzate in a yield of 33.8%.

Embodiment 3

[0080] Example 3: Preparation of hydrolyzate of soybean protein (3)

[0081] Soy protein was suspended so that defatted soybean powder (trade name PROPHAM974, manufactured by ADM FAR EAST Co., Ltd.) had a concentration of 5% (w / v), and pancreas was added to the suspension so that it became 5% (w / v). Protease was reacted at 50°C for 18 hours at pH 7.5, which is near the optimal pH of the protease, and then 0.05% (w / v) of Sumizyme FL-G (manufactured by Shin Nippon Chemical Co., Ltd.) The optimum pH near 7.0 was decomposed at 60°C for 18 hours.

[0082] After completion of the reaction, the above-mentioned reactant was heat-treated at 85° C. for 15 minutes to inactivate the enzyme contained in the reactant. Next, diatomaceous earth (Fuso Chemical, RADIOLITE #200) was precoated on the Buchner funnel, and suction filtration was performed. Next, the filtrate was concentrated under reduced pressure and freeze-dried to obtain powdered soybean protein hydrolyzate in a yield of 43.9%....

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Abstract

The purpose of the present invention is to provide a novel substance having an activity for promoting adhesion between a cell and collagen. The present invention pertains to the preparing of a composition for promoting adhesion between the cell and collagen by using a soy protein hydrolyzate.

Description

technical field [0001] The present invention relates to a composition for promoting adhesion between cells and collagen and / or for promoting contraction of collagen gel, and inventions related thereto. Background technique [0002] In the dermis of mammalian organisms represented by humans, the adhesion of fibroblasts to collagen in the extracellular matrix is ​​one of the reasons for strengthening and stabilizing the grid-like three-dimensional structure of collagen fiber bundles and maintaining the tension and elasticity of the skin . Furthermore, it is known that if the ability of fibroblasts to adhere to collagen decreases due to aging or the like, the tension and elasticity of the skin will decrease. Furthermore, it is known that if a scaffold (foot field) is obtained without adhering to collagen fibers, the actions of fibroblasts themselves (such as the production of various factors, etc.) are also slowed down. Furthermore, it is considered that the reduction of derm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61K8/64A61K8/97A61K38/00A61P17/00A61Q19/00
CPCA61K36/48A61K38/011A61K8/97A61Q19/08A61K8/9789A61P17/00
Inventor 阿部正通熊泽益德松本康助
Owner ROHTO PHARM CO LTD
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