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A culture substrate for promoting cross-infection of truffle mycorrhizal seedlings and its application method

A technology for cultivating substrates and mycorrhizal seedlings, which is applied in the directions of seed and rhizome treatment, botanical equipment and methods, cultivation, etc. species, the availability of raw materials, and the effect of shortening the time required for infection

Active Publication Date: 2016-11-16
PANZHIHUA FENGSHENGYUAN AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional inoculation technique is to inoculate a single sterile seedling with spore powder, which consumes a large amount of strains. For rare truffle seeds, especially European truffles, the price is too expensive
Cross-infection technology can effectively save strains, but there are still relatively few studies on cross-infection technology for cultivating truffle mycorrhizal seedlings. At present, there is no culture substrate specially used for cross-infection of truffle mycorrhizal seedlings, and the effect of cross-infection is also limited. still needs to be improved

Method used

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  • A culture substrate for promoting cross-infection of truffle mycorrhizal seedlings and its application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of Culture Substrate

[0031] The volume ratios of peat soil and perlite were 2:1, 7:3 and 3:1, respectively, and the two were evenly mixed to obtain three kinds of culture substrates, which were numbered No. 1, No. 2 and No. 3 respectively.

[0032] Take the above medium and add rooting powder, mix well, and control the quality of rooting powder to account for 0.1%~1% of the total mass of the culture medium. A series of culture substrates containing rooting powder were obtained, wherein the volume ratio of peat soil and perlite was 7:3, and the quality of rooting powder accounted for three types of culture substrates with a percentage of 0.01%, 0.05%, and 0.1% respectively in the total mass of the culture substrate. The culture substrates were numbered No. 4, No. 5 and No. 6 respectively.

[0033] Take the above two series of culture substrates containing rooting powder and without rooting powder, add water-retaining agent, mix evenly, control th...

Embodiment 2

[0036] The culture described in Example 1 is used for the cross-infection inoculation of truffle mycorrhizal seedlings, and No. 1-9 culture substrates are taken as 9 experimental groups respectively, and river sand: vermiculite: perlite=1:1:1 are taken respectively (volume ratio) mixed substrate and pure peat soil as control group 1 and control group 2, and each group was operated according to the following specific steps:

[0037] 1) Collection and processing of host plant seeds: collect and select high-quality host plant (Pine yunnanensis in this example) seeds for cleaning and sterilization;

[0038] 2) Collection and processing of truffle strains: collect mature truffle fruiting bodies, wash and sterilize, add water and crush into spore suspension;

[0039] 3) Sterilization of the culture medium: sterilize the above culture medium at 126°C for 3 hours, cool it down naturally, and place it in a sterile environment for one week before use. If it has been sterilized during pr...

Embodiment 3

[0047] According to the operation steps described in Example 2, in the step 2), the spore suspension is mixed with the water-retaining agent that has been made into water, and then sterile water is continuously added until the water-retaining agent reaches saturated water absorption capacity, so that the spores are suspended The liquid will be evenly adsorbed to the surface of the water retaining agent. In step 4) of No. 6 culture substrate, the pine species and the water-retaining agent that adsorbed the spore suspension were mixed and sown in the culture substrate, and the rest of the operations were the same as in Example 2. The statistical results of the infection rate show that the adoption method can increase the infection rate by 2-3% on the basis of the infection rate corresponding to each time period of No. 6 culture substrate in Example 2.

[0048] In the method described in this example, if step 4) is adjusted to: lay a 5 cm thick sterile culture substrate in a seed...

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Abstract

The invention discloses a culture medium for promoting cross infection among truffle mycorrhizas and a using method thereof. The culture medium contains peat and perlite with the volume ratio of 2 to 3: 1. The culture medium can supply adequate nutrition for seed germination and seedling root growth, is high in porosity, permeability and water retention, and is good for the development of root system, so that effect of promoting cross infection among truffle mycorrhizas is achieved; raw material is easy to obtain, and preparing method is simple; by using the method for promoting cross infection among truffle mycorrhizas with the culture medium, the amount of truffle can be saved greatly, effect of promoting cross infection among truffle mycorrhizas can be achieved, and time required by infection can be shortened greatly as compared with that of an existing culture method.

Description

technical field [0001] The invention belongs to the field of cultivation and planting of truffles, and relates to a culture substrate for promoting cross-infection of truffle mycorrhizal seedlings and a use method thereof. technical background [0002] As the world's top food, commercial truffles are highly respected in Europe. Since the 1960s, the Institut National de la Recherche Agronomique (INRA), the French National Agricultural Research Institute, and Italian scientists have jointly carried out truffle cultivation research to save the European truffle industry. After doing a lot of fruitful research work, scientists from the two countries have made breakthroughs, successfully cultivated mycorrhizal seedlings of truffles, and mastered a large number of technologies for multiplying high-quality mycorrhizal seedlings of truffles. Until 1975, Italian scientists produced black spore truffles for the first time in the world by cultivating truffle mycorrhizal seedlings in gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04A01G31/00A01C1/00
Inventor 简体平简瑕林强简维忠冉晓潇
Owner PANZHIHUA FENGSHENGYUAN AGRI DEV CO LTD
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