Carrier attached cyclic culture method of heterotrophic nitrification-aerobic denitrifying bacteria

Abstract

The invention discloses a carrier attached cyclic culture method of heterotrophic nitrification-aerobic denitrifying bacteria, wherein a light spherical filler having the diameter of 3cm is taken as a carrier, beer wastewater and various kinds of industrial wastewater are taken as a culture base fluid, and a culture device is composed of a main reactor and a pre-reactor. The pre-reactor is used for subculture by using the residual liquid left after the culture in the main reactor is finished; the unsaturated carrier is then transferred to the main reactor for further culture; the culture base liquid is utilized sufficiently and a pre-attachment process is provided for the carrier; therefore, the utilization rate of the culture medium and the attachment efficiency of the carrier are improved, and the self-control continuous operation of the two reactors is realized. The carrier attached cyclic culture method avoids too much loss of the bacteria; the bacterial can be saved more conveniently after being air-dried, high cell concentration is obtained, and the stability and endurance capacity of the bacteria are improved.

Description

technical field [0001] The invention belongs to a method for cultivating microbial bacterial agents for environmental engineering water treatment, in particular to a carrier-attached circulation culturing method for heterotrophic nitrification-aerobic denitrification bacterial agents. Background technique [0002] Circulation culture is a culture method in which cells are retained in a culture tank in some way for recycling, usually by sedimentation, centrifugation and membrane filtration. Each of these methods has some advantages and disadvantages. The sedimentation method has simple equipment and low energy consumption. However, due to the dense medium, especially in the later stage of cultivation, the organism and the medium are mixed together in a jelly-like shape, which is difficult to settle and the culture waste is difficult to separate. ;The centrifugation method is suitable for industrial culture media containing various particles, and is more suitable for large-sca...

AI Technical Summary

Problems solved by technology

Each of these methods has some advantages and disadvantages. The sedimentation method has simple equipment and low energy consumption. However, due to the dense medium, especially in the later stage of cultivation, the organism and the medium are mixed together in a jelly-like shape, which is difficult to settle and the culture waste is difficult to separate. ;The centrifugation method is suitable for industrial culture media containing various particles, and is more suitable for large-scale systems, but the process is more complicated and the energy consumption is higher; the membrane filtration method is more thorough in removing waste liquid, but the membrane is easily blocked, and pumps need to be added, etc. equipment, high energy consumption

These cyclic culture methods not only have some defects in the cultivation stage, but also have some problems in the preservation stage of the inoculum. Because the water content of the inoculum is high, and it is not easy to use methods such as air drying to remove moisture, the dry storage of the inoculum is more difficult.

Images