Method for cloning unknown flanking sequences of PS1 gene 5' of dianthus caryophyllus

A technology of unknown sequence and cloning method, applied in the field of bioengineering, can solve the problem of low expression of carnation PS1 gene, and achieve the effect of saving manpower, low cost and strong pertinence
CN103756997AInactive Publication Date: 2014-04-30YUNNAN YUNKE FLOWER +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YUNNAN YUNKE FLOWER
Publication Date
2014-04-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for cloning unknown flanking sequences of a PS1 gene 5' of dianthus caryophyllus, belonging to the technical field of bioengineering. According to the method, five degenerate primers and two specific primers are designed according to homologous sequences, and the unknown flanking sequences of the PS1 gene 5' of dianthus caryophyllus can be obtained by adopting nested PCR (Polymerase Chain Reaction) amplification. The method disclosed by the invention has the characteristics of simplicity and convenience in operation, few operating restrictions, long cloned fragments, strong pertinence, low cost and the like.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and relates to a method for determining the unknown 5' flank sequence based on the cloned partial DNA sequence of carnation PS1 gene by using degenerate primers and specific primers through nested PCR amplification technology. Background technique

[0002] Using 2n gametes for sexual polyploidy has higher adaptability and heterozygosity than asexual polyploidy, and is an effective polyploid breeding approach. We found that carnation (Dianthus caryophyllus L) is mostly diploid, and most of them can produce low-frequency 2n gametes. The abnormal spindle is the main reason for the formation of caryophyllus 2n gametes. In Arabidopsis, the PS1 gene regulates the orientation of the spindle, and mutations in the PS1 gene can produce high-frequency 2n gametes. The study of carnation PS1 gene will help to reveal the molecular genetic mechanism of 2n gamete formation, but the sequence of carnation ...

Claims

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