DNA (deoxyribonucleic acid) detection method for quickly distinguishing transgenic plant and product thereof

Abstract

The invention relates to a DNA (deoxyribonucleic acid) detection method for quickly distinguishing a transgenic plant and a product thereof, which is characterized by comprising the following steps: (1) extracting and purifying a known DNA transgenic plant; (2) selecting known DNA sequence in transgenic construction, and designing primers of one end of PCR (polymerase chain reaction); (3) designing degenerate oligonucleotide; (4) designing the PCR; (5) separating the PCR product by gel electrophoresis to obtain a specific gel electrophoresis spectrum corresponding to each transgenic plant; (6) extracting the DNA of the plant to be detected or product thereof, and amplifying transgenic and adjacent sequence to obtain a gel electrophoresis spectrum; and (7) comparing the plant to be detected or product thereof with the gel electrophoresis spectrum of the known transgenic plant. The invention provides a DNA detection method for quickly a distinguishing transgenic plant and a product thereof. The method is used for identifying the source of the transgenic product by utilizing the characteristic that genetic transformation randomly inserts a host genome.

Description

technical field [0001] The invention relates to the detection of the source of transgenic products by biological DNA detection technology, in particular to a DNA detection method for quickly distinguishing transgenic plants and their products. Background technique [0002] With the widespread application of genetic technology in agricultural production, a large number of genetically modified biological products have appeared on the market, causing unavoidable worries and anxieties among the public. Therefore, accurate detection of genetically modified products and tracing their sources have become an important step in the safety management of genetically modified products. [0003] Existing detection methods for genetically modified products are mainly to detect the genetically modified product itself. For example, the detection of insect-resistant transgenic corn is the detection of the presence of the transgenic Bacillus thuringiensis, cry gene; Existence (Agrobacterium ...

AI Technical Summary

Problems solved by technology

While such a test is effective in distinguishing between GM and non-GMO products, it cannot distinguish between several similar GM products

For example: it cannot differentiate between GM insect-resistant maize constructed by two different laboratories, because the GMs are both Bacillus thuringiensis toxin genes; nor can it distinguish between different species with the same GM, such as GM glyphosate-resistant soybean oil and GM glyphosate-resistant Phosphorus corn oil, because the transgenes are both bacterial EPSP genes; it also cannot distinguish between several transgenic lines produced in a laboratory, because these lines have the same transgene, but the insertion site of the transgene is different

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