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Multi-parameter paper-chip electrochemical immunosensor and method for detecting lung cancer markers

An immunosensor, paper chip technology, applied in scientific instruments, instruments, measuring devices, etc., can solve problems such as low sensitivity, and achieve the effects of high sensitivity, low reagent sample consumption, and high integration.

Active Publication Date: 2014-04-30
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problem of low sensitivity in the prior art, the object of the invention is to provide a paper chip electrochemical immunosensor modified with graphene-based composite nanomaterials

Method used

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  • Multi-parameter paper-chip electrochemical immunosensor and method for detecting lung cancer markers
  • Multi-parameter paper-chip electrochemical immunosensor and method for detecting lung cancer markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: Joint detection of NSE and ProGRP two parameters

[0057] A method for preparing and using a paper chip electrochemical immunosensor for small cell lung cancer detection using graphene-based composite nanomaterials, comprising the following steps:

[0058] (1) According to the combination of tumor markers recommended by the American Biochemical Society, two parameters, NSE and ProGRP, were selected for joint determination;

[0059] (2) Prepare foldable paper chip c: Print out the design pattern with four-channel sensitive regions 5 and four working electrodes 11' on Whatman1# filter paper with a wax spray printer, heat and melt the wax in an electronic temperature control instrument, and obtain Wax-patterned foldable paper chip c with hydrophilic regions and hydrophobic wax regions 4 appearing alternately; a double-sided adhesive cover layer a, a filter membrane b and a thin plastic mask with electrode patterns are made using a plotter, and the foldable A...

Embodiment 2

[0063] Example 2: Joint detection of three parameters of CEA, CYFRA21-1 and SCC

[0064] A method for preparing and using a paper chip electrochemical immunosensor for squamous cell carcinoma detection using graphene-based composite nanomaterials, comprising the following steps:

[0065] (1) According to the combination of tumor markers recommended by the American Biochemical Society, three parameters of CEA, CYFRA21-1 and SCC were selected for determination;

[0066](2) Prepare foldable paper chip c: Print out the design pattern with six channel sensitive regions 5 and six working electrodes 11' on Whatman1# filter paper with a wax spray printer, heat and melt the wax in an electronic temperature control instrument, and obtain A foldable paper chip c in which hydrophilic regions and hydrophobic wax regions 4 appear alternately; a double-sided adhesive cover layer a, a filter membrane b, and a thin plastic mask with electrode patterns are made by a plotter on the foldable pape...

Embodiment 3

[0070] Example 3: Joint detection of four parameters of CEA, CYFRA21-1, NSE and ProGRP

[0071] A method for preparing and using a paper chip electrochemical immunosensor for detecting lung cancer of unknown cause using graphene-based composite nanomaterials, comprising the following steps:

[0072] (1) According to the combination of tumor markers recommended by the American Biochemical Society, four parameters of CEA, CYFRA21-1, NSE and ProGRP were selected for determination;

[0073] (2) Prepare foldable paper chip c: Print out the design pattern with eight channel sensitive regions 5 and eight working electrodes 11' on Whatman1# filter paper with a wax jet printer, heat and melt the wax in an electronic temperature control instrument, and obtain A foldable paper chip c in which hydrophilic regions and hydrophobic wax regions 4 appear alternately; a double-sided adhesive cover layer a, a filter membrane b, and a thin plastic mask with electrode patterns are made by a plotte...

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Abstract

The invention provides a multi-parameter paper-chip electrochemical immunosensor which comprises a covering layer, a filtering film and a foldable paperchip, wherein the covering layer is provided with a sample introduction hole, and first comb teeth which are used for exposing the end of a metal lead wire on the foldable paperchip; the filtering film is closely attached to a position below the sample introduction hole to filter and intercept large cells in a sample; the foldable paperchip comprises a first filter paperchip, a second filter paperchip and a broken line located in a middle position, sensitive areas and central drainage holes which are formed in the first filter paper chip, and working electrodes and an Ag-AgCl electrodes which are arranged on the second filter paper chip along the broken line, are symmetrically distributed; craphene-metal composite nano-materials, electronicmediators and various antibodies are decorated in the sensitive areas. After specific recognition with various substances to be detected, the filter paper chip is folded to be used for detecting the electrochemical signal change caused by the substances to be detected in each sensitive area. The invention also provides a method for detecting multiple lung cancer markers.

Description

technical field [0001] The invention relates to the technical fields of paper chip production, nano modification and immunodetection, in particular to a multi-parameter paper chip electrochemical immunosensor modified by a graphene-based nanocomposite material and its application in lung cancer detection. Background technique [0002] Lung cancer is one of the most common malignant tumors, and its morbidity and mortality rate rank first among all kinds of malignant tumors. How to quickly and early diagnose is the key to improving the efficacy and cure rate of lung cancer. Tumor markers are substances secreted or shed by cancer cells into body fluids or tissues. Compared with traditional imaging examinations, their detection is cheap, non-invasive, and easy for patients to accept. However, a single marker is not yet sufficiently sensitive for the diagnosis of lung cancer. sex and specificity. The combined detection of multiple tumor markers is more valuable for the early dia...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N33/574
Inventor 罗金平蔡新霞王力宋轶琳石文韬刘军涛徐辉任
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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